Indigenous Seeds, Seed Selection and Seed Bank for Sustainable Agriculture

Indigenous seeds are grown by the farmers over the years with a strong influence from local natural factors. Such seeds have a higher level of intrapopulation variations and the capacity of buffering the adverse factors. Understanding indigenous seeds along with their diversity are useful to diversify their uses, to assess conservation status, to know the factors making farming areas red zone, and to improve their performance. Selection is the simplest and most common method for the improvement of crop varieties. The variation must be created and maintained to impose selection. Different types of selection can be considered depending on the mode of reproduction of crops. Response to selection and correlated response are estimated to make the selection process more effective. Many different selection approaches can target either developing monomorphic or polymorphic varieties. There are five selection units and can be applied in five crop stages. Farmers’ criteria need to be considered during selection process. Based on the genotypic classes, there are three types of selection namely stabilizing selection, directional selection, and disruptive selection. The most simple and common selection methods are pure lines, mass selection, and class-bulking selection. Orthodox seeds in short, medium, and long-term storage facilities are conserved as a seed bank. Major types are household seed banks, community seed banks, national seeds, natural seed banks, and global seed banks. A seed bank is for assuring the availability of crop diversity for research, study, and production. The common works in seed banks are diversity collection, regeneration, characterization, multiplication, and distribution along with online database management.

A Review on In-vitro Haploid Production in Orchids

The aim of writing this paper is to review production of haploids in orchids in vitro. Haploids possess half number of chromosomes and do not undergo fertilization. In vitro conditions provide necessary nutrients and conditions that are required for growth of haploid plantlets. The natural breeding cycle of orchids is very slow as well as unpredictable. To reduce this time, the technique of in vitro haploid production is used. In vitro conditions can decrease or shorten the time required for juvenile period in Orchids. Haploid plants are produced from in vitro haploid culture. This technique is useful to produce homozygous pure lines and to increase the yield of a plant.

Anadolu-T Etlik Piliç Saf Hatlarının Gelişme ve Karkas Özellikleri

This study aimed to determine the growth and carcass traits of Anadolu-T broiler pure lines, which were kept breeding and selection over five generations from 2017 to 2021, in comparison with a commercial hybrid. After the selection process, pure lines (A1, A2, A3, B1, B2) and commercial hybrid (Ross-308) eggs were incubated and 120 female-male chicks were produced from each genotype in each generation. The broiler chickens were reared in broiler production standards for 6 weeks and carcass traits were determined by slaughtering at the end of this period. In this study, growth traits such as body weight (BW), feed conversion ratio (FCR), and livability; breast and thigh meat ratio were considered as carcass traits. The highest average BW (2961.2 to 3481.2 g) and best FCR (1.43 to 1.60) at 6 weeks of age over the five generations occurred in Ross-308 commercial hybrids; followed by sire (BW: 2633.8 to 3180.2 g; FCR: 1.65 to 1.77), dam lines (BW: 2307.7 to 2698.5 g; FCR: 1.65 to 1.78), respectively. Although there were some numerical differences in livability between generations in pure lines, it was generally at a similar level (96-100%) with commercial hybrids. The breast ratio was lower than commercial hybrid in both dam and sire lines, but it was determined that the thigh ratio was higher in pure lines. The fact that pure lines have a short selection history of five generations does not allow for detailed evaluation. We expect that the use of individual feed efficiency test in dam and sire lines, and additionally using body weight and breast

DArTseq Genotypic and Phenotypic Diversity of Barley Landraces Originating from Different Countries

Landraces are considered a key element of agrobiodiversity because of their high variability and adaptation to local environmental conditions, but at the same time, they represent a breeding potential hidden in gene banks that has not yet been fully appreciated and utilized. Here, we present a genome-wide DArTseq analysis of the diversity of 116 spring barley landraces preserved in the collection of the Polish gene bank. Genetic analysis revealed considerable variation in this collection and several distinct groups related to the landraces’ country of origin and the grain type were identified. The genetic distinctness of hulless accessions may provide a basis for pro-quality breeding aimed at functional food production. However, the variable level of accession heterogeneity can be a significant obstacle. A solution to this problem is the establishment of special collections composed of pure lines that are accessible to breeders. Regions lacking genetic diversity have also been identified on 1H and 4H chromosomes. A small region of reduced heterogeneity was also present in the hulless forms in the vicinity of the nud gene that determines the hulless grain type. However, the SNPs present in this area may also be important in selection for traits related to grain weight and size because their QTLs were found there. This may support breeding of hulless forms of spring barley which may have applications in the production of high-quality foods with health-promoting values.

Study of the Source Material of Tomatoes, Obtaining Pure Lines and Organizing the Primary Seed Breeding

Tomato occupies a leading place, which, with a wide variety of crops grown in the republic, are of great importance in human nutrition. The research was carried out within the framework of the draft State Program the approved National Strategy “Conservation and rational use of genetic resources of cultivated plants” for 2017–2020 in the Republic of Azerbaijan, as in other branches of agriculture, is of great importance in the selection work carried out in the field of vegetable growing. Tomato, which occupies a leading place among a wide variety of vegetable crops grown in the republic, possesses great importance in human nutrition. Zoned varieties of tomatoes that differ in taste and aroma were threatened with extinction under the environmental impact factors, for a number of objective and subjective reasons, currently there are very few genetic variations of these varieties in farms or they are on the verge of extinction. The National Genbank stores the seeds of these tomato varieties, which are currently very valuable in tomato breeding as donor samples adapted to local conditions. In breeding, the gene pool is considered an indispensable material. An important task facing breeders in our time is the effective use of this invaluable “golden fund” in the creation of new, high-quality, locally adapted varieties and hybrids. The article deals with the use of samling and sorting operations in the selection programs by establishing pure lines for the recovery of vitality and life cycle of seeds of regional, high quality and productive tomato varieties protected in the National Gene Bank established under the National Academy of Sciences Institute of Genetics.

Prospects of using adaptive phagotherapy (Phage therapy) in the rehabilitation of post-Covid 19 patients (editorial).

The work is devoted to the organization of postcovid rehabilitation by developing a strategy of adative phage therapy, which is the production and application of a complex of bacteriophages for a specific medical institution/department based on an up-to-date collection of hospital bacterial strains isolated from the biomaterial of patients of the same institution. Bacteriophages were actively used in the world in the 20-40s of the twentieth century in various fields of medicine, but the rapid development of phage resistance in each individual case limited their use. The use of complex preparations of bacteriophages from the collection pure lines of bacteriophages of the SPC "MikroMir" a set of various phages aimed at restoring the human microbiome after a covid infection allowed to sharply reduce the number of antibiotics used in intensive care units and reduce antibiotic resistance with proven safety of phage therapy.

Assessment of Agricultural Valuable Indicators of Pure Lines of Tomato

The article is devoted to the study of several tomatoes introduced and created in Vegetable Growing Research Institute (Baku) and pure lines derived from them. Biological and economically-valuable characteristics of varieties and obtained pure are given. It should be noted that in Azerbaijan, 25 different varieties of tomatoes are introduced and localized. These varieties have been cultivated on farms for years and have lost their farm valuable qualities due to various biotic an abiotic factors. Although tomato is a self-pollinating plant, special selection and seeding measures are required for reproduction. Therefore, in order to restore the original characteristics of regionalized tomato varieties, it is necessary to re-study their biological characteristics and economically-valuable qualities. Our research was carried out in the direction of individual selection and acquisition of pure lines of typical plants with characteristic features and characteristics. In the course of the work, 2–3 best pure lines were separated from each variety. These lines differ from the parent varieties in terms of ripening time, fruit weight and productivity. Thus, as a result of experiments, it was determined that they give the product in 10–15 days, the characteristics of coexistence are also high, forming uniformity in shape, size and color. The obtained lines are promising, will be included in the selection of seed work as a valuable starting material, and will also be increased.

Histology of Microspores of Chile Apple Capsicum pubescens R and P. and in vitro Culture

In plant biotechnology, in vitro culture of gametic or sexual cells, microspores or pollen grains, has been described as a successful tool to accelerate genetic improvement, obtaining haploid, homozygotic plants or pure lines in a short time. In chile apple, Capsicum pubescens R and P. Anthers were sown in vitro, and their cytological analysis, locating the meiotic division stage of microspores or pollen grains. Flower buds with diameters from 2.5 to 4.4 mm were pre-incubated at 4°C, in ascorbic and citric acid at 100 and 150 mg-L-1 for 24 h. Five semisolid culture media (A1, A2, A3, A4 and A5) were used, with Murashige and Skoog (1962) salts (MS), modifying iron and vitamin chelates, sucrose, and L-cysteine, 2,4-dichlorophenoxyacetic acid (2,4-D) and Kinetin (Kin). Anthers, in vitro, were plated, in light and dark, for 70 days. Two differentiation media (R1 and R2) were evaluated with 100% MS salts, glycine, kinetin and myo-inositol. The anthers seeded, coincided with the first mitosis of the microspore, the anthers, formed callus in the media (A1) 100 % EDTA-Fe, 0.40 mg-L-1 thiamine, 3 % sucrose) and (A3) 100 % EDTA-Fe, 0.40 mg-L-1 thiamine, 3 % sucrose, 0. 3 mg-L-1 of 2,4-D, and differentiated pro-embryonic structures in (A3) and (A5) 200 % EDTA-Fe, 0.4 mg-L-1 thiamine, 50 mg-L-1 pyridoxine, folic acid, riboflavin and niacin, 0.3 mg-L-1 2,4-D plus 0.3 mg-L-1 Kinetin, as well as roots in (A1). Light influenced the formation of pro-embryos and roots, in the dark callus. The media (R1) and (R2) favored the formation of pro-embryos.

Doubled Haploids in Eggplant

Eggplant is a solanaceous crop cultivated worldwide for its edible fruit. Eggplant breeding programs are mainly aimed to the generation of F1 hybrids by crossing two highly homozygous, pure lines, which are traditionally obtained upon several self crossing generations, which is an expensive and time consuming process. Alternatively, fully homozygous, doubled haploid (DH) individuals can be induced from haploid cells of the germ line in a single generation. Several attempts have been made to develop protocols to produce eggplant DHs principally using anther culture and isolated microspore culture. Eggplant could be considered a moderately recalcitrant species in terms of ability for DH production. Anther culture stands nowadays as the most valuable technology to obtain eggplant DHs. However, the theoretical possibility of having plants regenerated from somatic tissues of the anther walls cannot be ruled out. For this reason, the use of isolated microspores is recommended when possible. This approach still has room for improvement, but it is largely genotype-dependent. In this review, we compile the most relevant advances made in DH production in eggplant, their application to breeding programs, and the future perspectives for the development of other, less genotype-dependent, DH technologies.