scholarly journals Antifungal activity of essential oils derived from some plants against phytopathogenic fungi

2016 ◽  
Vol 5 (07) ◽  
pp. 1374 ◽  
Author(s):  
Habung Yami ◽  
Shukla A.K.*
Keyword(s):  
Antifungal Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Phytopathogenic Fungi ◽  
Penicillium Expansum ◽  
Acorus Calamus ◽  
Colony Diameter ◽  
Erigeron Canadensis ◽  
Artemisia Nilagirica ◽  
Poisoned Food Technique

Essential oils were extracted from different plant species (Acorus calamus, Artemisia nilagerica, Erigeron Canadensis) to evaluate their effect on the growth of four phytopathogenic fungi viz. Alternaria alternata, Botrytis cinerea, Fusarium oxysporum and Penicillium expansum following poisoned food technique method. Different concentration of oil such as 125ppm, 250ppm, 500ppm, 1000ppm and 5000ppm were taken to evaluate the effect. There was 100% inhibition in the growth of phytopathogenic fungi at 5000 and 1000ppm concentration by essential oil of A. calamus. At 500ppm concentration also 100% inhibition was found up to 7th day on F. oxysporum. Essential oil of A. nilagirica inhibits the growth of all phytopathogenic fungi at higher concentration. In case of P. expansum at 5000ppm concentration 100% inhibition was recorded even after 15 days of incubation. The decrease in colony diameter or growth of fungus was corresponding to the concentration of oil. In comparison to others two, essential oil of Erigeron Canadensis was less effective against phytopathogenic fungi. It can be stated on the basis of results that the use of Acorus calamus and Artemisia nilagirica essential oil could be an alternative to synthetic fungicides for management of post harvest phytopathogenic fungal diseases caused by A. alternata, B. cinerea, F. oxysporum and P. expansum.

scholarly journals Composition Chimique Et Activité Antimicrobienne Des Huiles Essentielles De Deux Espèces De Lavande : Lavandula Dentata Spp. Dentata Et Lavandula Peduncultata Spp. Pedunculata

2017 ◽  
Vol 13 (21) ◽  
pp. 293
Author(s):  
Lamia Bachiri ◽  
Mohamed Bammou ◽  
Ghizlane Echchegadda ◽  
Jamal Ibijbijen ◽  
Lhoussaine El Rhaffari ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Essential Oils ◽  
Aerial Part ◽  
Phytopathogenic Fungi ◽  
Penicillium Expansum ◽  
Antibacterial And Antifungal Activity ◽  
Composition Chimique ◽  
Methanol Fraction ◽  
Aspergillus Brasiliensis ◽  
Antibacterial And Antifungal

The aim of the present work is to compare the chemical composition, antibacterial and antifungal activity of two spontaneous species of lavender "Lavandula stoechas L." and "Lavandula dentata L." in Morocco. A phytochemical screening was performed on the aerial part of invested plants. The qualitative variation was revealed in the secondary metabolites of the species studied. Subsequently, the dosage of the crude organic extracts showed that the polyphenol content was higher in the methanol fraction, with an important yield in L. dentata. Also, the essential oils of the aerial part, obtained by hydrodistillation gave higher yields in L. dentata than L. pedunculata. Finally, tests of the antibacterial and antifungal activity of the essential oils of the two species were performed on four strains of bacterial pathogens (Staphylococcus aureus "Gram+," and Escherichia coli; Klebsiella pneumoniae and Proteus mirabilis which are Gram-), and three phytopathogenic fungi : Rhizopus stolonifer, Aspergillus brasiliensis and Penicillium expansum, The results obtained showed that the essential oil of L. dentata showed a strong antibacterial activity against the studied strains, particularly against S. aureus. While the antifungal activity revealed that L. pedunculata has a remarkable antifungal power against the fungi tested in comparison with L. dentata.

Antifungal Activity of Essential Oil and Hydrosol Extract of Ballota nigra L. and their Protective Effects Against the Black Rot of Tomatoes

2019 ◽  
Vol 15 (7) ◽  
pp. 662-671 ◽  
Author(s):  
Nabila A. Sebaa ◽  
Amina T. Zatla ◽  
Mohammed E.A. Dib ◽  
Boufeldja Tabti ◽  
Jean Costa ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Antifungal Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Alternaria Alternata ◽  
Penicillium Expansum ◽  
Protective Effects ◽  
Antifungal Activities

Background: Bellota species are used to treat various diseases in traditional folk medicine. Objectives: This study aimed to chemically characterize the essential oils and the hydrosol extract and regional specificity of the major components of Ballota nigra essential oil and to evaluate their in vitro and in vivo antifungal activities. Methods: Essential oils were obtained by a Clevenger-type apparatus and analyzed by using Gas Chromatography (GC) and Gas Chromatography Mass Spectroscopy (GC/MS). The antifungal activities were tested to three phytopathogenic stains (Penicillium expansum, Aspergillus niger and Alternaria alternata). Results: Altogether, 38 compounds were identified in the essential oils, representing 92.1-96.8% of the total oil composition. Their main constituents were E-β-caryophyllene (4.8-24.6%), E-β-farnesene (3.3-22.9%), β-bisabolene (7.6-30.2%), α-humulene (2.1-13.3%) and geranyl linalool (1.1-8.2%). The statistical methods deployed confirmed that there is a relation between the essential oil compositions and the harvest locations. Hydrosol extract was constituted by seven components, represented principally by methyl eugenol (75.2%) and caryophyllene oxide (12.5%). The results of in vitro antifungal activity with essential oil and hydrosol extract have shown very interesting antifungal activities on Penicillium expansum and Alternaria alternata strains with percentage reductions up to 80%. Additionally, in in vivo assays, Ballota nigra essential oil and hydrosol extract significantly reduce decay in artificially inoculated tomato by Alternaria alternata. Conclusion: The essential oil and hydrosol extract can be used as a potential source of sustainable eco-friendly botanical fungicides to protect stored tomatoes from pathogens, saprophytic fungi causing bio-deterioration to a variety of food commodities.

scholarly journals THE ESSENTIAL OIL CONTENTS OF JERINGAU (Acorus calamus L.) RHIZOMES AND THEIR ANTIFUNGAL ACTIVITY AGAINST Candida albicans

2017 ◽  
Vol 1 (1) ◽  
pp. 33 ◽  
Author(s):  
Wiwik Susanah Rita ◽  
Retno Kawuri ◽  
I Made Dira Swantara
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Steam Distillation ◽  
Inhibitory Concentration ◽  
Diffusion Method ◽  
Acorus Calamus ◽  
Oil Concentration ◽  
Trans Methyl

Acorus calamus L. rhizome was trusted having antibacterial activity. This study aimed to identify the compounds in the Acorus Calamus L. rhizomes essential oils and to recognize the antifungal activity of the oils against Candida albicans. The extraction of essential oils from rhizome was carried out by steam distillation technique. Identification of compounds in the oils was conducted by Gas Chromatography- Mass Spectroscopy (GCMS), while the antifungal test against Candida albicans was done by well diffusion method. Extraction of 10 kg of rhizomes produced 16.53 mL essential oil with a yield of 0.1653% (? = 1.066), the oil was brownish yellow and very flavorful. GC-MS analysis showed that the essential oil contained 11 compounds, they are (E)-3,7 dimethyl-1,3,6-Octatriene (trans-?-Ocimene) (3,73%), linalool (1,07%), ?-elemene (1,15%), trans methyl isoeugenol (7,68%), shyobunon (15,74%), bicyclogermakren (0,93%), dehidroxy-isocalamendiol (2,61%), ?-calacorene (3,34%), euasarone (26,84), cis-asarone (18,62%); dan trans- asarone (18,29%). Antifungal activity test showed that the growth and biomass inhibition of C. albicans increased with the increase of the oil concentration. Minimum Inhibitory Concentration (MIC) of essential oil toward C. albicans was 1% with the inhibition of 7.83 mm.

scholarly journals Influence of eight chosen essential oils in the vapor phase on the growth of Rhizopus stolonifer and Rhizopus lyococcus

10.5219/1586 ◽  
2021 ◽  
Vol 15 ◽  
pp. 378-386
Author(s):  
Dana Tančinová ◽  
Miroslava Hlebová ◽  
Denisa Folitinova ◽  
Zuzana Mašková ◽  
Zuzana Barboráková
Keyword(s):  
Antifungal Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Vapor Phase ◽  
Quantitative Composition ◽  
Acorus Calamus ◽  
Citrus Paradisi ◽  
Rhizopus Stolonifer ◽  
Bitter Orange ◽  
Star Anise

This study aimed to evaluate the fungicidal effect of eight essential oils against five strains of the genus Rhizopus. Strains were obtained from various moldy foods,  namely Rhizopus stolonifer KMi 383 from chestnut, R. stolonifer KMi 510 from strawberry, R. stolonifer KMi 511 from nectarine, R. stolonifer KMi 524 from cherry tomatoes, and R. lyococcus KMi 512 from blackberry. The essential oils (EO) used in this study were jasmine EO (extract from Jasminum officinale L.), bergamot EO (Mentha aquatica L. var. citrata (Her.) Fresen), bitter orange EO (from Citrus aurantium L.), grapefruit EO (Citrus paradisi Macfady), sweet flag EO (East Asian Calamus, from Acorus calamus L. var. angustatus Bes), star anise EO (from Illicium verum J.D.Hook), geranium EO (from Pelargonium graveolens), and lemongrass EO (from Cymbopogon citratus DC). The semi-quantitative composition of the essential oil samples was determined by gas chromatography coupled with mass spectrometry (GC-MS). The antifungal activity of essential oils against the strains of R. stolonifer and R. lyococcus was determined during 7 days, using the micro-atmosphere method (0.625 μL.mL-1 of air). Two essential oils, geranium and lemongrass, completely inhibited the growth of all isolates. Bitter orange essential oil inhibited the growth of all isolates of Rhizopus stolonifer, but isolate of Rhizopus lyococcus began to grow after four days of cultivation. In conclusion, certain essential oils are highly effective in the vapor phase. These could be used in further tests of their antifungal activity and could be used in the control of Rhizopus spp. or other fungal pathogens.

scholarly journals In Vitro and In Vivo Inhibition of Cylindrocladium reteaudii by Essential Oils of Acorus calamus Rhizomes

2020 ◽  
Vol 19 (1) ◽  
pp. 34-42
Author(s):  
Phanin Sintawarak ◽  
◽  
Suwimon Uthairatsamee ◽  
Tharnrat Keawgrajang ◽  
◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Fungal Growth ◽  
Antifungal Compound ◽  
Acorus Calamus ◽  
In Vivo Experiments ◽  
Oil Concentration

Cylindrocladium reteaudii (Bugnic.) Boesew. is a severe pathogen which can cause leaf blight disease in Eucalyptus seedlings in tropical countries. This study investigated the antifungal activity of essential oils extracted from Acorus calamus L. rhizomes in inhibiting the growth of C. reteaudii, both in in vitro and in vivo experiments. The extraction of essential oils from rhizomes was carried out by hydro-distillation technique and the in vitro antifungal testing was done by using the poisoned food technique. The results indicated that an essential oil concentration of 2,000 ppm can completely inhibit the fungal growth with a 50% inhibitory concentration value of 54.76 ppm. For the in vivo experiment, it was found that an essential oil concentration of 500 ppm and Captan® of 1,000 ppm were not significantly different in inhibiting the growth of C. reteaudii. However, these two treatments significantly inhibited the fungal growth (p<0.05) when compared with the control treatments. Physiological and anatomical characteristics were investigated to check for the antifungal activity after the application of essential oils. Results showed that essential oil spraying had no effect on the leaf transpiration rate and temperature of the Eucalyptus seedlings, but the incident disease ratio was high when an essential oil concentration of more than 1,500 ppm was applied. Therefore, it can be inferred that the essential oils from A. calamus rhizomes at an optimum concentration can be an efficient antifungal compound with a potential to control leaf and shoot blight diseases in Eucalyptus seedlings in a nursery.

scholarly journals Antifungal activity of essential oils on mycelial growth of Fusarium oxysporum and Bortytis cinerea

2019 ◽  
pp. 544 ◽  
Author(s):  
Marina Palfi ◽  
Paško Konjevoda ◽  
Karolina Vrandečić
Keyword(s):  
Antifungal Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Fusarium Oxysporum ◽  
Mycelial Growth ◽  
In Vitro Study ◽  
Phytopathogenic Fungi ◽  
Phytopathogenic Fungus ◽  
Antifungal Effect

In vitro study of the effect of different volumes of twelve essential oils on the mycelial growth of economically significant phytopathogenic fungi (Fusarium oxysporum and Botrytis cinerea) and it was compared to the effect of a fungicide. The antifungal activity of essential oils is decreased with the duration of incubation and it differs depending on the type of phytopathogenic fungus and the applied volume. The most effective antifungal effect on both tested fungi was in the essential oil of thyme, with lowest values of IC50 while the weakest effect was in essential oils of eucalyptus and lemon, with the highest values of IC50. Certain essential oils, when applied in certain volumes, had the same or even better effect on the inhibition of the growth of mycelium when compared to the tested fungicides.

Biological Activities of Carlina Oxide Isolated from the Roots of Carthamus caeruleus

2020 ◽  
Vol 10 (2) ◽  
pp. 145-152 ◽  
Author(s):  
Imane Rihab Mami ◽  
Rania Belabbes ◽  
Mohammed El Amine Dib ◽  
Boufeldja Tabti ◽  
Jean Costa ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Essential Oil ◽  
Skin Diseases ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Penicillium Expansum ◽  
Natural Food ◽  
Antioxidant Power

Background: Carthamus caeruleus belongs to the Asteraceae family. The roots are traditionally used as healing agents. They help to heal burns and treat skin diseases. They are also used against joint inflammation and are very effective against diseases such as irritable bowel syndrome for cancer patients. Objectives: The purpose of this work was i) to study the chemical composition of i) the essential oil and hydrosol extract of Carthamus caeruleus, ii) to isolate the major component of both extracts and iii) to evaluate their antioxidant, antifungal and insecticidal activities. Methods: The essential oil and hydrosol extract obtained from the roots were studied by GC and GC/MS. The antioxidant activities were performed using two different methods i) Radical scavenging activity (DPPH) and ii) the Ferric-Reducing Antioxidant Power (FRAP), using BHT as a positive control. Whereas, the antifungal activity of the essential oil and Carlina oxide was investigated against plant fungi. The fumigation toxicity of C. caeruleus essential oil besides Carlina oxide was evaluated against adults of Bactrocera oleae better known as the olive fly. Results: The essential oil and hydrosol extract were mainly represented by acetylenic compounds such as carline oxide and 13-methoxy carline oxide. Carlina oxide was isolated and identified by 1H and 13C NMR spectroscopic means. The results showed that Carlina oxide presented interesting antioxidant and antifungal properties, while C. caeruleus root essential oil had better insecticidal activity. Furthermore, Carlina oxide has demonstrated promising in vivo antifungal activity to control infection of apples by Penicillium expansum. Conclusion: Carlina oxide can be used as a natural food preservative and alternative to chemical fungicides to protect stored apple against Penicillium expansum.

scholarly journals Nanoencapsulated Essential Oils with Enhanced Antifungal Activity for Potential Application on Agri-Food, Material and Environmental Fields

Antibiotics ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 31
Author(s):  
Magdaléna Kapustová ◽  
Giuseppe Granata ◽  
Edoardo Napoli ◽  
Andrea Puškárová ◽  
Mária Bučková ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Winning Strategy ◽  
Colloidal Suspensions ◽  
Fungal Species ◽  
Natural Environments ◽  
Food Material ◽  
Minimum Fungicidal Concentration ◽  
Fungal Strains

Nanotechnology is a new frontier of this century that finds applications in various fields of science with important effects on our life and on the environment. Nanoencapsulation of bioactive compounds is a promising topic of nanotechnology. The excessive use of synthetic compounds with antifungal activity has led to the selection of resistant fungal species. In this context, the use of plant essential oils (EOs) with antifungal activity encapsulated in ecofriendly nanosystems could be a new and winning strategy to overcome the problem. We prepared nanoencapsules containing the essential oils of Origanum vulgare (OV) and Thymus capitatus (TC) by the nanoprecipitation method. The colloidal suspensions were characterized for size, polydispersity index (PDI), zeta potential, efficiency of encapsulation (EE) and loading capacity (LC). Finally, the essential oil nanosuspensions were assayed against a panel of fourteen fungal strains belonging to the Ascomycota and Basidiomycota phyla. Our results show that the nanosystems containing thyme and oregano essential oils were active against various fungal strains from natural environments and materials. In particular, the minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) values were two to four times lower than the pure essential oils. The aqueous, ecofriendly essential oil nanosuspensions with broad-spectrum antifungal activity could be a valid alternative to synthetic products, finding interesting applications in the agri-food and environmental fields.

scholarly journals Chemical composition and antifungal activity of essential oils from four Asteraceae plants grown in Egypt

2018 ◽  
Vol 73 (7-8) ◽  
pp. 313-318 ◽  
Author(s):  
Rose Vanessa Bandeira Reidel ◽  
Simona Nardoni ◽  
Francesca Mancianti ◽  
Claudia Anedda ◽  
Abd El-Nasser G. El Gendy ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Antifungal Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Inhibitory Activity ◽  
First Time

Abstract The objective of the present paper was the assessment of the chemical composition of the essential oils from four Asteraceae species with a considerable food, medicinal, and agricultural value, collected in Egypt, together with their in vitro inhibitory activity against molds and yeasts. The essential oil of Launaea cornuta flowers was also evaluated for the first time, but because of its very low yield (<0.01%), no antifungal test was performed.

scholarly journals Chemical Compositions, Phytotoxicity, and Biological Activities of Acorus Calamus Essential Oils from Nepal

2013 ◽  
Vol 8 (8) ◽  
pp. 1934578X1300800 ◽  
Author(s):  
Prabodh Satyal ◽  
Prajwal Paudel ◽  
Ambika Poudel ◽  
Noura S. Dosoky ◽  
Debra M. Moriarity ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Aspergillus Niger ◽  
Seed Germination ◽  
Essential Oils ◽  
Brine Shrimp ◽  
Biological Activities ◽  
Acorus Calamus ◽  
Chemical Compositions ◽  
Seedling Growth Inhibition ◽  
Mcf 7

Four essential oils from the leaf (P23) and rhizomes (P19, P22, P24) of Acorus calamus L., collected from various parts of Nepal, were obtained by hydrodistillation and analyzed by GC-MS. From a total of 61 peaks, 57 compounds were identified among the four essential oils accounting for 94.3%, 96.2%, 97.6%, and 94.1% of the oils, respectively. All of the essential oils were dominated by ( Z)-asarone (78.1%–86.9%). The essential oils also contained ( E)-asarone (1.9%–9.9%) and small amounts of γ-asarone (2.0–2.3%), ( Z)-methyl isoeugenol (1.5–2.0%), and linalool (0.2–4.3%). Allelopathic testing of the rhizome oil showed inhibition of seed germination of Lactuca sativa and Lolium perenne with IC50 values of 450 and 737 μg/mL, respectively. The rhizome essential oil demonstrated stronger seedling growth inhibition of L. perenne than of L. sativa, however. The rhizome oil also showed notable brine shrimp lethality ( LC50 = 9.48 μg/mL), cytotoxic activity (92.2% kill on MCF-7 cells at 100 μg/mL), and antifungal activity against Aspergillus niger (MIC = 19.5 μg/mL).

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