Fluorescent Modification Of N-Terminal Amino Group In Peptides For Complete Sequence Analysis Using Psd Method In Maldi-Tof-Ms

2004 ◽  
Vol 11 (1) ◽  
pp. 71-77 ◽  
Author(s):  
Masatoshi Nakagawa ◽  
Hiroshi Nakanishi
Keyword(s):  
Sequence Analysis ◽  
Complete Sequence ◽  
Amino Group ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Terminal Amino ◽  
Tof Ms

scholarly journals Evaluation of the performance of MALDI-TOF MS and DNA sequence analysis in the identification of mycobacteria species

2018 ◽  
Vol 48 (6) ◽  
pp. 1351-357 ◽  
Author(s):  
Işın AKYAR ◽  
Cengiz ÇAVUŞOĞLU ◽  
Meltem AYAŞ ◽  
Süheyla SÜRÜCÜOĞLU ◽  
Arzu İLKİ ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Dna Sequence ◽  
Dna Sequence Analysis ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

GyrB sequence analysis and MALDI-TOF MS as identification tools for plant pathogenic Clavibacter

2011 ◽  
Vol 34 (6) ◽  
pp. 400-407 ◽  
Author(s):  
Joanna Zaluga ◽  
Kim Heylen ◽  
Koenraad Van Hoorde ◽  
Bart Hoste ◽  
Johan Van Vaerenbergh ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Gyrb Sequence ◽  
Tof Ms

scholarly journals Species Identification of Campylobacter jejuni and Campylobacter coli Isolates from Raw Poultry Products by MALDI–TOF MS and rRNA Sequence Analysis

2020 ◽  
Vol 103 (1) ◽  
pp. 197-204 ◽  
Author(s):  
Irshad M Sulaiman ◽  
Ying-Hsin Hsieh ◽  
Steven Simpson
Keyword(s):  
Sequence Analysis ◽  
Species Identification ◽  
Rrna Sequence ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Poultry Products ◽  
Ms Analysis ◽  
Raw Poultry ◽  
Vitek Ms ◽  
Tof Ms

Abstract Background: Campylobacter is a genus of Gram-negative bacteria. It is considered one of the leading cause of diarrheal illness worldwide. Incidence of human campylobacteriosis has increased significantly during recent decades; the increase has been directly linked with the advancement made in Campylobacter detection methods. C. jejuni and C. coli are the two major human-pathogenic species that can colonize poultry and cause foodborne illness and outbreak. Objective: This study was carried out to rapidly identify Campylobacter-like isolates recovered from raw poultry products by matrix-assisted laser desorption ionization (MALDI)–time-of-flight (TOF) MS and DNA sequencing of 16S and 23S ribosomal RNA (rRNA) regions. Methods: Twenty-seven isolates of Campylobacter-like organisms were isolated from raw poultry products and cultured on blood agars for MALDI–TOF MS analysis and DNA isolation. For each isolate, one to two colonies were directly spotted on the VITEK MS analysis. Genomic DNA was extracted from overnight bacterial culture. Afterward, two-directional Sanger sequencing of rRNA gene was performed to confirm species identification on an ABI 3500xL Genetic Analyzer. Results: The VITEK MS could provide species-level identification for all 27 Campylobacter-like bacterial isolates (including 13 isolates as C. jejuni and 14 isolates as C. coli). Species identification attained by the VITEK MS matched completely with the rRNA sequence characterization data. Conclusions: Based on the restricted number of target strains and agar plates, MALDI–TOF MS and rRNA sequence analysis can be used for rapid identification of C. jejuni and C. coli isolates. Highlights: MALDI–TOF MS and rRNA sequencing can provide species identification of C. jejuni and C. coli isolates.

895: Proteomic Analysis of Sera from Bladder Cancer Patients with MALDI-TOF-MS

2007 ◽  
Vol 177 (4S) ◽  
pp. 297-297
Author(s):  
Kristina Schwamborn ◽  
Rene Krieg ◽  
Ruth Knüchel-Clarke ◽  
Joachim Grosse ◽  
Gerhard Jakse
Keyword(s):  
Bladder Cancer ◽  
Cancer Patients ◽  
Proteomic Analysis ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

HPTLC-MALDI/TOF/MS vs. HPLC-ESI/MS2 for identification to flavonoids in plant extracts

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
L Fougère ◽  
D Da Silva ◽  
E Destandau ◽  
C Elfakir
Keyword(s):  
Plant Extracts ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

Infektionserreger beim Schwein – Analyse des kultivierbaren Mikrobioms mittels MALDI-TOF MS

2017 ◽  
Author(s):  
M Erhard ◽  
M Metzner ◽  
D Köhler-Repp ◽  
B Köhler ◽  
R Storandt
Keyword(s):  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms
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