scholarly journals RNase T1 mediated base-specific cleavage and MALDI-TOF MS for high-throughput comparative sequence analysis

2003 ◽  
Vol 31 (9) ◽  
pp. 47e-47 ◽  
Author(s):  
R. Hartmer
Keyword(s):  
Sequence Analysis ◽  
High Throughput ◽  
Comparative Sequence Analysis ◽  
Maldi Tof Ms ◽  
Comparative Sequence ◽  
Maldi Tof ◽  
Tof Ms

scholarly journals Evaluation of the performance of MALDI-TOF MS and DNA sequence analysis in the identification of mycobacteria species

2018 ◽  
Vol 48 (6) ◽  
pp. 1351-357 ◽  
Author(s):  
Işın AKYAR ◽  
Cengiz ÇAVUŞOĞLU ◽  
Meltem AYAŞ ◽  
Süheyla SÜRÜCÜOĞLU ◽  
Arzu İLKİ ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Dna Sequence ◽  
Dna Sequence Analysis ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

Finding diabetic nephropathy biomarkers in the plasma peptidome by high-throughput magnetic bead processing and MALDI-TOF-MS analysis

2010 ◽  
Vol 4 (8-9) ◽  
pp. 697-705 ◽  
Author(s):  
Henning G. Hansen ◽  
Julie Overgaard ◽  
Maria Lajer ◽  
Frantisek Hubalek ◽  
Peter Højrup ◽  
...  
Keyword(s):  
Diabetic Nephropathy ◽  
High Throughput ◽  
Magnetic Bead ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Ms Analysis ◽  
Tof Ms

ChemInform Abstract: Novel Propargylamine-Linked Nucleosides for High Throughput SNP Genotyping by MALDI-TOF MS

ChemInform ◽  
2010 ◽  
Vol 32 (48) ◽  
pp. no-no
Author(s):  
Thomas Wenzel ◽  
Thomas Froehlich ◽  
Kathrin Strassburger ◽  
Susann Richter ◽  
Jacqueline Bimmler ◽  
...  
Keyword(s):  
High Throughput ◽  
Snp Genotyping ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

scholarly journals High-throughput identification of the microbial biodiversity of cocoa bean fermentation by MALDI-TOF MS

2016 ◽  
Vol 63 (5) ◽  
pp. 347-355 ◽  
Author(s):  
S. Miescher Schwenninger ◽  
S. Freimüller Leischtfeld ◽  
C. Gantenbein-Demarchi
Keyword(s):  
High Throughput ◽  
Cocoa Bean ◽  
Microbial Biodiversity ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Cocoa Bean Fermentation ◽  
Tof Ms

scholarly journals Development of an Optimized MALDI-TOF-MS Method for High-Throughput Identification of High-Molecular-Weight Glutenin Subunits in Wheat

Molecules ◽  
2020 ◽  
Vol 25 (18) ◽  
pp. 4347
Author(s):  
You-Ran Jang ◽  
Kyoungwon Cho ◽  
Se Won Kim ◽  
Susan B. Altenbach ◽  
Sun-Hyung Lim ◽  
...  
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
High Throughput ◽  
Glutenin Subunits ◽  
Maldi Tof Ms ◽  
Molecular Weights ◽  
Maldi Tof ◽  
Rp Hplc ◽  
Group 2 ◽  
Tof Ms

Because high-molecular-weight glutenin subunits (HMW-GS) are important contributors to wheat end-use quality, there is a need for high-throughput identification of HMW-GS in wheat genetic resources and breeding lines. We developed an optimized method using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) to distinguish individual HMW-GS by considering the effects of the alkylating reagent in protein extraction, solvent components, dissolving volume, and matrix II components. Using the optimized method, 18 of 22 HMW-GS were successfully identified in standard wheat cultivars by differences in molecular weights or by their associations with other tightly linked subunits. Interestingly, 1Bx7 subunits were divided into 1Bx7 group 1 and 1Bx7 group 2 proteins with molecular weights of about 82,400 and 83,000 Da, respectively. Cultivars containing the 1Bx7 group 2 proteins were distinguished from those containing 1Bx7OE using well-known DNA markers. HMW-GS 1Ax2* and 1Bx6 and 1By8 and 1By8*, which are difficult to distinguish due to very similar molecular weights, were easily identified using RP-HPLC. To validate the method, HMW-GS from 38 Korean wheat varieties previously evaluated by SDS-PAGE combined with RP-HPLC were analyzed by MALDI-TOF-MS. The optimized MALDI-TOF-MS method will be a rapid, high-throughput tool for selecting lines containing desirable HMW-GS for breeding efforts.

Sign in / Sign up

Export Citation Format

Share Document