Production of polyhydroxyalkanoate (PHA) by Ralstonia eutropha JMP 134 with volatile fatty acids from palm oil mill effluent as precursors

2015 ◽  
Vol 72 (11) ◽  
pp. 1889-1895 ◽  
Author(s):  
Tjandra Setiadi ◽  
Martha Aznury ◽  
Azis Trianto ◽  
Adi Pancoro
Keyword(s):  
Fatty Acids ◽  
Palm Oil ◽  
Volatile Fatty Acids ◽  
Ralstonia Eutropha ◽  
Anaerobic Fermentation ◽  
Palm Oil Mill Effluent ◽  
Fed Batch ◽  
Batch Mode ◽  
Pha Production ◽  
Palm Oil Mill

The highest volatile fatty acids (VFAs) concentration from palm oil mill effluent (POME) treated by anaerobic fermentation was achieved for a 1-day process when the main acids used were acetic, propionic and butyric acids. Polyhydroxyalkanoate (PHA) production with VFAs from POME as precursors in the fed-batch mode has advantages over batch mode, both in terms of its productivity and 3HV (3-hydroxyvalerate) composition in the produced polymer. With the fed batch, the productivity increased to 343% and contained more 3HV than those of the batch. The structures of the PHA were identified by different methods and they supported each other; the resulting products consisted of functional groups of 3HB (3-hydroxybutyrate) and 3HV.

scholarly journals Identifikasi Senyawa Asam Lemak Volatil dari Air Limbah Industri Minyak Kelapa Sawit untuk Produksi Polihidroksialkanoat oleh Ralstonia eutropha JMP 134

2021 ◽  
Vol 11 (01) ◽  
pp. 49
Author(s):  
Martha Aznury ◽  
Tjandra Setiadi
Keyword(s):  
Fatty Acids ◽  
Palm Oil ◽  
Industrial Wastewater ◽  
Volatile Fatty Acids ◽  
Ralstonia Eutropha ◽  
Bacterial Fermentation ◽  
Dry Cell Weight ◽  
Pha Production ◽  
Palm Oil Mill ◽  
Dry Cell

Identification of Volatile Fatty Acids from Palm Oil Mill Effluent for Polyhydroxyalkanoate Production by Ralstonia eutropha JMP 134 Abstract Polyhydroxyalkanoate (PHA) is a bioplastic which is derived from bacterial fermentation. In this study, PHA is produced by utilizing Ralstonia eutropha JMP 134 and volatile fatty acids (VFA) from palm oil industrial wastewater as a precursor. The aim of this research is to study the effect of carbon source, addition time, and VFA concentration on PHA production by fermentation using Ralstonia eutropha JMP 134 in batch. PHA and dry cell weight (DCW) concentrations obtained by adding VFA from palm oil industrial wastewater in batches at 20 and 40 hours were 0.014 g/L.hour, 2.76 g/L and 3.66 g/L, respectively. The results also showed that the time of adding VFA greatly affected cell growth, with the best addition time being after the 20th hour. Keywords: palm oil industrial wastewater, polyhydroxyalkanoate (PHA), batch, Ralstonia eutropha JMP 134, volatile fatty acids (VFA) Abstrak Polihidroksialkanoat (PHA) adalah bioplastik yang diproses melalui proses fermentasi dengan mikroba. Pada penelitian ini, PHA diproduksi dengan menggunakan Ralstonia eutropha JMP 134 dan memanfaatkan asam lemak volatil (ALV) dari air limbah industri minyak kelapa sawit sebagai prekursor. Penelitian ditujukan mempelajari pengaruh sumber karbon, waktu penambahan, dan konsentrasi ALV terhadap  poduksi PHA yang difermentasi menggunakan Ralstonia eutropha JMP 134 secara batch. Konsentrasi PHA dan berat kering sel (BKS) yang diperoleh pada penambahan ALV dari air limbah industri kelapa sawit secara batch pada jam ke-20 dan 40 masing-masing bernilai 0,014 g/L.jam,  2,76 g/L dan 3,66 g/L. Hasil penelitian memperlihatkan pula bahwa waktu penambahan ALV sangat mempengaruhi pertumbuhan sel, dengan waktu penambahan yang terbaik adalah setelah pada jam ke-20.  Kata kunci: air limbah industri minyak kelapa sawit, polihidroksialkanoat (PHA), batch, Ralstonia eutropha JMP 134, asam lemak volatil (ALV)  

scholarly journals Utilization of palm oil mill effluent and clindamycin for optimization of polyhydroxy [r] alkanoates production

2019 ◽  
Vol 10 (1) ◽  
pp. 4740-4746
Keyword(s):  
Palm Oil ◽  
Volatile Fatty Acids ◽  
Production Condition ◽  
Nile Blue ◽  
Carbon Sources ◽  
Palm Oil Mill Effluent ◽  
Blue Staining ◽  
Storage Granules ◽  
Pha Production ◽  
Palm Oil Mill

Polyhydroxyalkanoates (PHA) are storage granules of most bacteria which can be used as biodegradable plastics but the production cost of PHA is twice than petrochemical based synthetic polymers because of substrate cost. The use of alternative renewable and cheap carbon sources are the best option, one such is palm oil mill effluent (POME). POME contains carbon source like volatile fatty acids and other organic components which can be utilised by microorganisms to accumulate PHA. The use of subinhibitory concentration of antibiotics like clindamycin may have an influence on PHA accumulation. In this study, 31 organisms were isolated from POME spillage area and subjected to PHA production. Seven organisms were found to accumulate PHA, which was confirmed by Nile blue staining method, the accumulated PHA was extracted and characterized using HPLC. All the organisms were found to produced poly hydroxy butyrate (PHB). Amongst all the seven isolates, two organisms namely Bacillus sp and Pseudomonas aeruginosa were found to accumulate more PHA. Both the organisms were subjected to produce PHA in POME and clindamycin containing media. PHA production condition was optimized using RSM.

Effects of even and odd number fatty acids cofeeding on PHA production and composition inPseudomonas putidaBet001 isolated from palm oil mill effluent

2015 ◽  
Vol 63 (1) ◽  
pp. 92-100 ◽  
Author(s):  
Mohd Rafais Mohd Razaif-Mazinah ◽  
Mohamad Suffian Mohamad Annuar ◽  
Yusrizam Sharifuddin
Keyword(s):  
Fatty Acids ◽  
Palm Oil ◽  
Palm Oil Mill Effluent ◽  
Pha Production ◽  
Palm Oil Mill

Pilot-scale of biohythane production from palm oil mill effluent by two-stage thermophilic anaerobic fermentation

2019 ◽  
Vol 44 (6) ◽  
pp. 3347-3355 ◽  
Author(s):  
Jiravut Seengenyoung ◽  
Chonticha Mamimin ◽  
Poonsuk Prasertsan ◽  
Sompong O-Thong
Keyword(s):  
Palm Oil ◽  
Anaerobic Fermentation ◽  
Pilot Scale ◽  
Palm Oil Mill Effluent ◽  
Two Stage ◽  
Palm Oil Mill

scholarly journals Fed batch production of hydrogen from palm oil mill effluent using anaerobic microflora

2005 ◽  
Vol 30 (13-14) ◽  
pp. 1393-1397 ◽  
Author(s):  
A ATIF ◽  
A FAKHRULRAZI ◽  
M NGAN ◽  
M MORIMOTO ◽  
S IYUKE ◽  
...  
Keyword(s):  
Palm Oil ◽  
Palm Oil Mill Effluent ◽  
Fed Batch ◽  
Batch Production ◽  
Production Of Hydrogen ◽  
Palm Oil Mill ◽  
Anaerobic Microflora

scholarly journals Pengaruh sumber karbon terhadap produksi bioplastik polihidroksialkanoat (PHA) dengan ralstonia eutropha

2018 ◽  
Vol 9 (1) ◽  
pp. 28
Author(s):  
Martha Aznury ◽  
Tjandra Setiadi ◽  
Adi Pancoro
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Carbon Source ◽  
Fermentation Process ◽  
Volatile Fatty Acids ◽  
Ralstonia Eutropha ◽  
Operating Conditions ◽  
Batch Bioreactor ◽  
Pha Production ◽  
Fructose Fermentation

Bioplastic Polyhidroxyalknoate (PHA) is a polyester type bioplastic with physicochemical properties resemble to those of polypropilen from petroleum. PHA production was investigated to determine the effect of carbon source on the fermentation process by Ralstonia eutropha. Specifically, Ralstonia eutropha was cultivated in a batch bioreactor to show the dynamics of P(3HB-co-3HV) copolymer production from glucose or fructose as C source. In adition, the effect of volatile fatty acids addition, as stimulator to the copolymer production, was also studied. The operating conditions in a 7 L bioreactor were at temperature 30 oC and pH 7.0. The concentration of carbon source glucose or fructose was 40 g/L, and after 20 hour fermentation, volatile fatty acids were added. With volatile fatty acids addition, the resulting fructose fermentation had PHA content of 32.78%, in which the HV percentage was 11.78%. Meanwhile, the fermentation of glucose, stimulated by volatile fatty acids, gave PHA as much as 20.19% with HV percentage of 8.71%. Therefore,, the Ralstonia eutropha fermentation of fructose as the carbon source gave a higher yield than glucose. Keywords: Volatil Fatty Acid, Fructose, Glucose, PHA, P(3HB-co-3HV), Ralstonia eutropha AbstrakBioplastik polihidroksialkanoat (PHA) adalah bioplastik dari kelompok poliester dengan sifat fisikokimia mirip dengan plastik polipropilen dari minyak bumi. Penelitian ini bertujuan untuk mempelajari pengaruh sumber karbon terhadap poduksi PHA yang dilakukan dengan proses fermentasi menggunakan Ralstonia eutropha. Ralstonia eutropha dikultivasi dalam bioreaktor batch untuk mempelajari dinamika produksi kopolimer P(3HB-co-3HV) dari sumber karbon glukosa atau fruktosa, serta mempelajari pengaruh sumber stimulator asam lemak volatil. Kondisi operasional fermentasi menggunakan bioreaktor 7 L adalah pada temperatur 30 oC dan pH 7. Konsentrasi sumber karbon glukosa atau fruktosa yang digunakan adalah 40 gr/L, dan setelah 20 jam fermentasi ditambahkan asam lemak volatil yang berfungsi sebagai stimulator dalam produksi P(3HB-co-3HV). Panen sel Ralstonia eutropha dilakukan setelah 60 jam. Hasil penelitian menunjukkan fermentasi Ralstonia eutropha dengan substrat fruktosa dan asam lemak volatil sebagai stimulator mempunyai kandungan PHA sebesar 32,78%, dengan kadar HV 11,78%. Pada pemberian substrat glukosa dan asam lemak volatil menunjukkan kandungan PHA sebesar 20,19%, dengan kadar HV 8.71%. Jadi fermentasi Ralstonia eutropha dengan menggunakan substrat fruktosa memberikan yield yang lebih tinggi dibandingkan menggunakan substrat glukosa.Kata Kunci: Asam lemak volatil, fruktosa, glukosa, PHA, P(3HB-co-3HV), Ralstonia eutropha

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