Effects of even and odd number fatty acids cofeeding on PHA production and composition inPseudomonas putidaBet001 isolated from palm oil mill effluent

2015 ◽  
Vol 63 (1) ◽  
pp. 92-100 ◽  
Author(s):  
Mohd Rafais Mohd Razaif-Mazinah ◽  
Mohamad Suffian Mohamad Annuar ◽  
Yusrizam Sharifuddin
Keyword(s):  
Fatty Acids ◽  
Palm Oil ◽  
Palm Oil Mill Effluent ◽  
Pha Production ◽  
Palm Oil Mill

Production of polyhydroxyalkanoate (PHA) by Ralstonia eutropha JMP 134 with volatile fatty acids from palm oil mill effluent as precursors

2015 ◽  
Vol 72 (11) ◽  
pp. 1889-1895 ◽  
Author(s):  
Tjandra Setiadi ◽  
Martha Aznury ◽  
Azis Trianto ◽  
Adi Pancoro
Keyword(s):  
Fatty Acids ◽  
Palm Oil ◽  
Volatile Fatty Acids ◽  
Ralstonia Eutropha ◽  
Anaerobic Fermentation ◽  
Palm Oil Mill Effluent ◽  
Fed Batch ◽  
Batch Mode ◽  
Pha Production ◽  
Palm Oil Mill

The highest volatile fatty acids (VFAs) concentration from palm oil mill effluent (POME) treated by anaerobic fermentation was achieved for a 1-day process when the main acids used were acetic, propionic and butyric acids. Polyhydroxyalkanoate (PHA) production with VFAs from POME as precursors in the fed-batch mode has advantages over batch mode, both in terms of its productivity and 3HV (3-hydroxyvalerate) composition in the produced polymer. With the fed batch, the productivity increased to 343% and contained more 3HV than those of the batch. The structures of the PHA were identified by different methods and they supported each other; the resulting products consisted of functional groups of 3HB (3-hydroxybutyrate) and 3HV.

scholarly journals Utilization of palm oil mill effluent and clindamycin for optimization of polyhydroxy [r] alkanoates production

2019 ◽  
Vol 10 (1) ◽  
pp. 4740-4746
Keyword(s):  
Palm Oil ◽  
Volatile Fatty Acids ◽  
Production Condition ◽  
Nile Blue ◽  
Carbon Sources ◽  
Palm Oil Mill Effluent ◽  
Blue Staining ◽  
Storage Granules ◽  
Pha Production ◽  
Palm Oil Mill

Polyhydroxyalkanoates (PHA) are storage granules of most bacteria which can be used as biodegradable plastics but the production cost of PHA is twice than petrochemical based synthetic polymers because of substrate cost. The use of alternative renewable and cheap carbon sources are the best option, one such is palm oil mill effluent (POME). POME contains carbon source like volatile fatty acids and other organic components which can be utilised by microorganisms to accumulate PHA. The use of subinhibitory concentration of antibiotics like clindamycin may have an influence on PHA accumulation. In this study, 31 organisms were isolated from POME spillage area and subjected to PHA production. Seven organisms were found to accumulate PHA, which was confirmed by Nile blue staining method, the accumulated PHA was extracted and characterized using HPLC. All the organisms were found to produced poly hydroxy butyrate (PHB). Amongst all the seven isolates, two organisms namely Bacillus sp and Pseudomonas aeruginosa were found to accumulate more PHA. Both the organisms were subjected to produce PHA in POME and clindamycin containing media. PHA production condition was optimized using RSM.

scholarly journals Identifikasi Senyawa Asam Lemak Volatil dari Air Limbah Industri Minyak Kelapa Sawit untuk Produksi Polihidroksialkanoat oleh Ralstonia eutropha JMP 134

2021 ◽  
Vol 11 (01) ◽  
pp. 49
Author(s):  
Martha Aznury ◽  
Tjandra Setiadi
Keyword(s):  
Fatty Acids ◽  
Palm Oil ◽  
Industrial Wastewater ◽  
Volatile Fatty Acids ◽  
Ralstonia Eutropha ◽  
Bacterial Fermentation ◽  
Dry Cell Weight ◽  
Pha Production ◽  
Palm Oil Mill ◽  
Dry Cell

Identification of Volatile Fatty Acids from Palm Oil Mill Effluent for Polyhydroxyalkanoate Production by Ralstonia eutropha JMP 134 Abstract Polyhydroxyalkanoate (PHA) is a bioplastic which is derived from bacterial fermentation. In this study, PHA is produced by utilizing Ralstonia eutropha JMP 134 and volatile fatty acids (VFA) from palm oil industrial wastewater as a precursor. The aim of this research is to study the effect of carbon source, addition time, and VFA concentration on PHA production by fermentation using Ralstonia eutropha JMP 134 in batch. PHA and dry cell weight (DCW) concentrations obtained by adding VFA from palm oil industrial wastewater in batches at 20 and 40 hours were 0.014 g/L.hour, 2.76 g/L and 3.66 g/L, respectively. The results also showed that the time of adding VFA greatly affected cell growth, with the best addition time being after the 20th hour. Keywords: palm oil industrial wastewater, polyhydroxyalkanoate (PHA), batch, Ralstonia eutropha JMP 134, volatile fatty acids (VFA) Abstrak Polihidroksialkanoat (PHA) adalah bioplastik yang diproses melalui proses fermentasi dengan mikroba. Pada penelitian ini, PHA diproduksi dengan menggunakan Ralstonia eutropha JMP 134 dan memanfaatkan asam lemak volatil (ALV) dari air limbah industri minyak kelapa sawit sebagai prekursor. Penelitian ditujukan mempelajari pengaruh sumber karbon, waktu penambahan, dan konsentrasi ALV terhadap  poduksi PHA yang difermentasi menggunakan Ralstonia eutropha JMP 134 secara batch. Konsentrasi PHA dan berat kering sel (BKS) yang diperoleh pada penambahan ALV dari air limbah industri kelapa sawit secara batch pada jam ke-20 dan 40 masing-masing bernilai 0,014 g/L.jam,  2,76 g/L dan 3,66 g/L. Hasil penelitian memperlihatkan pula bahwa waktu penambahan ALV sangat mempengaruhi pertumbuhan sel, dengan waktu penambahan yang terbaik adalah setelah pada jam ke-20.  Kata kunci: air limbah industri minyak kelapa sawit, polihidroksialkanoat (PHA), batch, Ralstonia eutropha JMP 134, asam lemak volatil (ALV)  

Development of a sequential injection-liquid microextraction procedure with GC-FID for analysis of short-chain fatty acids in palm oil mill effluent

Talanta ◽  
2017 ◽  
Vol 165 ◽  
pp. 612-618 ◽  
Author(s):  
Thapanee Pruksatrakul ◽  
Pattamaporn Phoopraintra ◽  
Prapin Wilairat ◽  
Pimchai Chaiyen ◽  
Rattikan Chantiwas
Keyword(s):  
Fatty Acids ◽  
Palm Oil ◽  
Short Chain Fatty Acids ◽  
Short Chain ◽  
Palm Oil Mill Effluent ◽  
Sequential Injection ◽  
Palm Oil Mill ◽  
Liquid Microextraction ◽  
Chain Fatty Acids

Bio-Synthesis of Eicosapentaenoic Acid (EPA) from Palm Oil Mill Effluent Using Anaerobic Process

2018 ◽  
Vol 382 ◽  
pp. 286-291
Author(s):  
Hanifrahmawan Sudibyo ◽  
Yano Surya Pradana ◽  
Budhijanto ◽  
Wiratni Budhijanto
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Palm Oil ◽  
Acidic Condition ◽  
Palm Oil Mill Effluent ◽  
Omega 3 Fatty Acids ◽  
Omega 3 ◽  
Epa And Dha ◽  
Palm Oil Mill ◽  
Pre Treatment

The supply of omega-3 fatty acids, which are EPA and DHA, was only 14.7% of the prospective demand calculated by using 500 mg/day/person as threshold limit for EPA and DHA consumption for a world population of 7.515 billion people. Alternative sources other than fish oil or seed-based-α linolenic acid (ALA) were necessary. Anaerobic digestion of palm oil mill effluent (POME) offered a new way for one of omega-3 fatty acids production, EPA. The digestion was conducted in anaerobic fluidized bed reactor supported by natural zeolite as the immobilization media. Process condition tried to be optimized was the pH level and the pre-treatment method (TSS filtration) before the digestion. Without any pre-treatment, the fatty acid profile produced after digestion contained considerable amount of polyunsaturated fatty acid with acidic condition provided higher EPA purity. On the other hand, pre-treatment led the digestion to produce saturated fatty acids with acidic condition provided very high purity of butyric acid as the product.

Development of Bio-PORec® system for polyhydroxyalkanoates (PHA) production and its storage in mixed cultures of palm oil mill effluent (POME)

2012 ◽  
Vol 124 ◽  
pp. 208-216 ◽  
Author(s):  
Mohd Fadhil Md. Din ◽  
Ponraj Mohanadoss ◽  
Zaini Ujang ◽  
Mark van Loosdrecht ◽  
Salmiati Muhd Yunus ◽  
...  
Keyword(s):  
Palm Oil ◽  
Mixed Cultures ◽  
Palm Oil Mill Effluent ◽  
Pha Production ◽  
Palm Oil Mill

scholarly journals Impregnated Zeolite as Catalyst in Esterification Treatment from High Free Fatty Acids Palm Oil Mill Effluent

2021 ◽  
Vol 16 (1) ◽  
pp. 19-27
Author(s):  
Ika Kusuma Nugraheni ◽  
Nuryati Nuryati ◽  
Anggun Angkasa Bela Persada ◽  
Triyono Triyono ◽  
Wega Trisunaryanti
Keyword(s):  
Fatty Acids ◽  
Free Fatty Acids ◽  
Palm Oil ◽  
Excessive Amount ◽  
Raw Material ◽  
Biodiesel Production ◽  
Palm Oil Mill Effluent ◽  
Palm Oil Mill

Although Palm Oil Mill Effluent (POME) can be used as a raw material for biodiesel production, the POME contains an excessive amount of Free Fatty Acids (FFA), necessitating a preliminary process and esterification. POME is degummed using phosphate acid and bleached with carbon active. Additionally, this study used KOH-impregnated zeolite to reduce FFA. The purpose of this study is to determine the effect of adding impregnated zeolite on esterification. POME was heated to 600C for 30 minutes, then degumming with 3 % of phosphate acid for 30 minutes, followed by bleaching with carbon active with a comparison ratio of 8:3 at 1000C for 1 hour, and finally, esterification with 3 percent impregnated zeolite from the POME weight at 600C for 4 hours. The analysis was conducted using titration methods to determine the FFA of each esterification. The data will be compared between zeolite and non-zeolite degumming, bleaching, and esterification. The results indicated that the most effective method for reducing FFA was degumming, bleaching, and esterification with zeolite.

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