Features of vaginal microbiocenosis in women of reproductive age with overweight and obesity

Overweight and obesity play a negative role in gynecological and obstetric practicE.In women, the frequency of infectious pathology increases against the background of metabolic disorderS.The most common form of infectious vaginitis is bacterial urogenital candidiasis, in the etiological structure of which a significant role belongs to the fungi Candida albicans, as well as Candida non-albicans: C.glabrata, C.tropicalis, C.parapsilosis, C.krusei. Associations of Candida fungi with various representatives of opportunistic microflora, such as gram-positive and gram-negative aerobic, facultative-anaerobic and obligate-anaerobic microorganisms, are often formed. As a result, numerous bacterial pathogens multiply and the number of lactobacilli, which are usually part of the bacterial flora of the vagina, is significantly reduced. In bacterial vaginosis (BV), the concentration of anaerobic pathogens Peptostreptococcus sp, Gardnerella vaginalis, Peptostreptococcus Mobiluncus sp, Mycoplasma hominis can increase 100 timeS.Activation of Atopobium vaginae and Gardnerella vaginalis, which play a “key” role in the pathogenesis of BV, has been proven. The aim of the study was to study changes in the vaginal microbiome in women with candidiasis and bacterial vaginosis in order to improve existing treatment regimenS.We examined 120 women of reproductive age with overweight and obesity. The degree of microbial contamination was determined and the maximum possible spectrum of aerobic and facultative-anaerobic microflora was detected. In women with vulvovaginal candidiasis, overweight and obesity, a high concentration (lg5.8 CFU/ml) of Candida fungi was found, and in 95% of patients two-, three- and four-component associations of Candida fungi with various representatives of conditional pathogenic microflora. Lactobacillus deficiency was found in 58.3% of patients, and their complete absence – in 10.0%. Bacteriological examination of the vaginal contents of women with vaginosis and obesity revealed significant dysbiotic disorders of the vaginal microflora, three-, four- and even five-component associations of anaerobic and facultative anaerobic microflora with a predominance of anaerobeS.A low seeding level of lactobacilli (lg2.2 CFU/ml) was established. Thus, the gram-positive anaerobic and facultative anaerobic microflora of Firmicutes have a significant share in the spectrum of vaginal microflora in overweight and obese patients, in contrast to non-obese women of reproductive agE.In women of reproductive age with vulvovaginal candidiasis and obesity, in contrast to non-obese patients, a higher frequency of fungal-bacterial associations, a higher quantitative level of vaginal contamination by Candida albicans and non-albicans with a lack or general absence of lactoflora.

Influence of ozone generated by pulsed alternating electric current on the viability of representatives of oral microflora involved in the development of prosthetic stomatitis

Topicality. When using removable dentures, in 84.26±1.19 % of cases patients have various changes in the mucous membrane of the prosthetic bed with pronounced changes in microbial ecology which are exacerbated by poor oral hygiene and removable dentures. Ozone therapy is a new and promising area of treatment of microbial lesions of the oral mucosa. It has antiseptic, anti-inflammatory, analgesic, immunomodulatory effects and promotes activation of intracellular metabolism, improvement of rheological properties and microcirculation of blood; it also strengthens reparative ability of the injured tissues. The aim of the study is to evaluate the in vitro effect of ozone generated by the device for use in dental practice on the survival of the representatives of aerobic and facultative anaerobic microflora of the oral cavity, which are important in the development of prosthetic stomatitis. Materials and methods of research. In vitro experiments helped to study the effect of ozone generated by pulsed alternating electric current (Ozone DTA device for use in dental practice) on the survival of the representatives of aerobic and facultative anaerobic microflora of the oral cavity, which may be potential factors in inflammatory processes in prosthetic bed tissues and the development of prosthetic stomatitis. The results were processed by the method of variation statistics and two-way analysis of variance ANOVA (MS Excel 2003). Results. A direct dependence of the influence of the survival level of S. aureus in suspension on the ozone generation capacity and the time of exposure to ozone was established. Different types of oral microorganisms differ in their sensitivity to ozone. Cultures of S. epidermidis, β-hemolytic Streptococcus group G, α-hemolytic streptococci S. sanguinis and S. oralis, yeast-like fungi Candida tropicalis are characterized by relatively higher sensitivity to the generated ozone. Conclusion. The obtained experimental results microbiologically substantiate the possibility and expediency of using ozone therapy to prevent the occurrence and treatment of prosthetic stomatitis. Based on the performed experiments, the optimal mode of ozone therapy with the Ozone DTA device was established to ensure the antimicrobial effect at the level of 98.8–100%. Key words: ozone, microorganisms, antimicrobial action, oral cavity.

GANGRENOUS MASTITIS IN A COW

Udder gangrene in an animal occurs when mastitis or udder wounds are complicated, in which anaerobic microflora is introduced into the udder, as well as through the ingestion of this microflora by galactogenic route and through the circulatory system by metastases