Biological decolorization of industrial dyes by Candida tropicalis and Bacillus firmus

2011 ◽  
Vol 63 (4) ◽  
pp. 761-768 ◽  
Author(s):  
Sucharita Arora ◽  
Harvinder Singh Saini ◽  
Kamaljit Singh
Keyword(s):  
Candida Tropicalis ◽  
Bacterial Culture ◽  
Cellulose Triacetate ◽  
Domestic Sewage ◽  
Soil Samples ◽  
Environmental Concerns ◽  
Disperse Dyes ◽  
Yeast Culture ◽  
Bacillus Firmus ◽  
Textile Industries

Disperse dyes are chiefly used by textile industries for the coloration of polyester and cellulose triacetate and their blended fibres. Their extensive use and recalcitrant nature, high tinctorial strength renders the voluminous textile effluents intensively colored and causes environmental concerns. Decolorization of representative members of Dianix CC and Dianix S brands (DyStar Pvt. Ltd.) of disperse dyes were tested with Candida tropicalis and Bacillus firmus isolated respectively from contaminated soil samples and sludge of a domestic sewage drain. While both the cultures efficiently remove color from the aqueous solutions of the dyes, the yeast culture was found to decolorize most of the tested disperse dyes through biotransformation, the bacterial culture showed color removal mainly by adsorption on the cell pellets. Formation of cleavage products such as p-nitroaniline was observed in the case of Dianix Orange E-3R, indicating reductive cleavage of the azo linkage of the dye.

scholarly journals Studies on decolorization of textile dye by using Pseudomonas and bacillus sp from the contaminated effluent soil samples of Kovilpatti, Thoothukudi district of Tamil Nadu

2019 ◽  
Vol 11 (1) ◽  
pp. 134-137 ◽  
Author(s):  
Kannan D ◽  
Renuga Devi ◽  
A. G. Murugesan ◽  
S. Rajan
Keyword(s):  
Contaminated Soil ◽  
Tamil Nadu ◽  
Soil Samples ◽  
Toxic Chemicals ◽  
Textile Dye ◽  
Pseudomonas Sp ◽  
Bacillus Sp ◽  
Bacterial Strains ◽  
Textile Industries ◽  
Source Of Pollution

Textile industries releasing large amount of effluent which contains textile dyes and toxic chemicals and it is one of the major source of pollution also contaminating water bodies. To remove that, bacteria have been of great attention because of their ability to treat effluent. The present study was undertaken to exploit the ability of Pseudomonassp and Bacillus sp from dye contaminated soil samples for bioremediation for dye effluent. Among the bacterial strains used in the study. Pseudomonas sp emerged out to be most potent decolorizer in comparison to Bacillus sp with the degree of decolorization of 90.0 %. Thus, it was concluded that the Pseudomonas sp had highest color removing capacity from contaminated effluent soil samples. 

scholarly journals Identification of Anthrax in Endemic Areas in South Sulawesi Province

2018 ◽  
Vol 2 (2) ◽  
Author(s):  
Sartika Juwita ◽  
Purwanta Purwanta ◽  
Muflihanah Muflihanah ◽  
Titis Furi Djatmikowati
Keyword(s):  
Bacillus Anthracis ◽  
Bacterial Culture ◽  
Virulent Strain ◽  
Lethal Factor ◽  
Soil Samples ◽  
Genome Chromosome ◽  
South Sulawesi ◽  
Dna Fragment ◽  
Endemic Areas ◽  
Bacillus Genome

Anthrax is a strategic and important disease because it is a zoonotic disease which caused death and difficult to eradicate because it produces spores. The aim of the study was to identify anthrax in endemic areas in South Sulawesi Province. Soil samples obtained from Gowa and Pinrang regency were examined by bacterial culture test and then positive results from bacterial culture followed by multiplex PCR. Based on the results of the isolation of 52 soil samples show 35 samples from Pinrang regency show all negative anthrax, while from 17 soil samples from Gowa regency show 2 positive samples of anthrax. Positive samples of anthrax were from Timbuseng village, Patallasang district, Gowa regency. The results continued with PCR technique using Bacillus anthracis colonies derived from positive soil samples. It showed the virulent strain of Bacillus anthracis. A virulent strain is indicated by a DNA fragment of a pXO1 plasmid encoding a toxin lethal factor (Lef) of 385 bp and a DNA fragment of a pXO2 plasmid encoding a capsule of anthrax (Cap) of 264 bp and also common bacterial markers of the Bacillus genome chromosome (Ba183) of 152 bp.

scholarly journals Dyeing of hydrophobic fabrics with disperse dyes

2001 ◽  
Vol 66 (6) ◽  
pp. 367-376 ◽  
Author(s):  
Hari Maradiya ◽  
Vithal Patel
Keyword(s):  
Absorption Spectroscopy ◽  
Elemental Analysis ◽  
Cellulose Triacetate ◽  
Spectral Studies ◽  
Disperse Dyes ◽  
Fastness Properties ◽  
Visible Absorption ◽  
Dyed Fabrics ◽  
Yellowish Orange ◽  
Visible Absorption Spectroscopy

A series of disperse dyes has been synthesized by diazotisation of 2,6-dibromo-4-nitroaniline and coupled with various N-arylmaleimides. The dyes were characterized by IR spectral studies, visible absorption spectroscopy and elemental analysis. All the dyes were applied as disperse dyes on nylon, cellulose triacetate and polyester fabrics. These dyeswere found to give yellowish orange to deep brown shades with very good depth, levelness and brightness on different fabrics. The percentage dye bath exhaustion and fixation on fabrics were found to be very good. The light, washing, rubbing, perspiration and sublimation fastness properties of the dyed fabrics were found to be good to excellent.

scholarly journals Effects of bacterial cultures, enzymes and yeast-based feed additive combinations on ruminal fermentation in a dual-flow continuous culture system

2021 ◽  
Author(s):  
S L Bennett ◽  
J A Arce-Cordero ◽  
V L N Brandao ◽  
J R Vinyard ◽  
B C Agustinho ◽  
...  
Keyword(s):  
Repeated Measures ◽  
Volatile Fatty Acids ◽  
Bacterial Culture ◽  
Nutrient Digestibility ◽  
Feed Additives ◽  
Feed Additive ◽  
Yeast Culture ◽  
Ruminal Fermentation ◽  
Bacterial Cultures ◽  
Molar Proportion

Abstract Bacterial cultures, enzymes and yeast derived feed additives are often included in commercial dairy rations due to their effects on ruminal fermentation. However, the effects of these additives when fed together are not well understood. The objective of this study was to evaluate the changes in ruminal fermentation when a dairy ration is supplemented with combinations of bacterial probiotics, enzymes and yeast. Our hypotheses were that ruminal fermentation would be altered, indicated through changes in volatile fatty acid profile and nutrient digestibility, with inclusion of: (1) an additive, (2) yeast and (3) increasing additive doses. Treatments were randomly assigned to 8 fermenters in a replicated 4 × 4 Latin square with four 10 d experimental periods, consisting of 7 d for diet adaptation and 3 d for sample collection. Basal diets contained 52:48 forage:concentrate and fermenters were fed 106 g of dry matter per day divided equally between 2 feeding times. Treatments were: control (CTRL, without additives); bacterial culture/enzyme blend (EB, 1.7 mg per day); bacterial culture/enzyme blend with a blend of live yeast and yeast culture (EBY, 49.76 mg per day); and double dose of the EBY treatment (2X, 99.53 mg per day). The bacterial culture/enzyme blend contained five strains of probiotics (Lactobacillus animalis, Propionibacterium freudenreichii, Bacillus lichenformis, Bacillus subtillis, and Enterococcus faecium) and three enzymes (amylase, hemicellulase, and xylanase). On d 8-10, samples were collected for pH, redox, volatile fatty acids, lactate, ammonia N and digestibility measurements. Statistical analysis was performed using the GLIMMIX procedure of SAS. Repeated measures were used for pH, redox, VFA, NH3-N and lactate kinetics data. Orthogonal contrasts were used to test the effect of: (1) additives, ADD (CTRL vs EB, EBY and 2X); (2) yeast, YEAST (EB vs EBY and 2X); and (3) dose, DOSE (EBY vs 2X). No effects (p > 0.05) were observed for pH, redox, NH3-N, acetate, isobutyrate, valerate, total VFA, acetate:propionate, nutrient digestibility or N utilization. Within the 24h pool, the molar proportion of butyrate increased (p = 0.03) with the inclusion of additives when compared to the control while the molar proportion of propionate tended to decrease (p = 0.07). In conclusion, inclusion of bacterial cultures, enzymes and yeast to the diet increased butyrate concentration; but did not result in major changes in ruminal fermentation.

scholarly journals Isolation of alkalophilic CGTase-producing bacteria and characterization of cyclodextrin-glycosyltransferase

2003 ◽  
Vol 46 (2) ◽  
pp. 183-186 ◽  
Author(s):  
lma Hiroko Higuti ◽  
Simone Wichert Grande ◽  
Roberta Sacco ◽  
Aguinaldo José do Nascimento
Keyword(s):  
Activation Energy ◽  
Enzyme Activity ◽  
Sugar Cane ◽  
Soil Samples ◽  
Optimum Temperature ◽  
Bacillus Firmus ◽  
Cyclodextrin Glycosyltransferase ◽  
Biochemical Tests ◽  
Starch Adsorption

One hundred and twenty five soil samples were collected from the regions of roots of corn, cassava, potato, bean, sugar cane, soya, and pumpkin. From these, 75 strains were isolated that produced a yellowish halo surrounding the colonies, due to a phenolphtalein-cyclodextrin (CD) complex, and these were selected as alkalophilic CGTase-producing bacteria. All the 75 strains were identified as Bacillus firmus by microscopy and biochemical tests. The activity of the CGTase's varied from 2² to 2(10) dilutions,when assayed by CD-trichloroethylene (TCE)-complex precipitation. Strain 31 that produced the enzyme at the higher level was selected, and its enzyme was partially purified by starch adsorption (x 17) in a yield of 51%. Maximum enzyme activity occurred at pH 5.5 and 8.5. At pH 5.5, the optimum temperature was 60°C. On increased from 30°C to 85°C, the thermodynamic parameter for activation energy was 8.27 kcal.mol-1. The enzyme was inhibited by Ca2+, Mg2+, Fe2+, Cu2+, Mn2+, and Zn2+.

scholarly journals Bio-treatment of soil from international airport in Ostrava

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Peter Fečko ◽  
Radmila Kučerová ◽  
Eva Pertile ◽  
Lucie Nezvalová ◽  
Nikolas Mucha ◽  
...  
Keyword(s):  
Pseudomonas Putida ◽  
Bacterial Culture ◽  
Laboratory Culture ◽  
Soil Samples ◽  
Mixed Bacterial Culture ◽  
International Airport ◽  
Pure Bacterial Culture ◽  
Rhodococcus Sp

The paper deals with an examination of possible application of biodegradation in the decontamination of soil samples from international airport in Ostrava. The laboratory biodegradation tests were carried out with a pure bacterial culture of Pseudomonas putida, a pure laboratory culture of Rhodococcus sp, their mixture and a mixture prepared combining their media free of bacteria. The results of the paper imply that for biodegradation of airport pollutants is most suitable to apply a mixed bacterial culture of Pseudomonas putida and Rhodococcus sp. The results show that the biodegradation method is applicable for the pollution.

scholarly journals Microbiological Assessment of Poultry Droppings, Water and Soil Under Deep Litter (Dl) And Battery Cage (Bl) Systems Within Lagos, Nigeria

2021 ◽  
Vol 6 (1) ◽  
pp. 80-98
Author(s):  
Hilda Emmanuel-Akerele ◽  
Paul Adamolekun
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Aspergillus Niger ◽  
Candida Tropicalis ◽  
Soil Samples ◽  
Percentage Occurrence ◽  
Poultry Farms ◽  
Aspergillus Sp ◽  
Poultry Droppings ◽  
Staphylococcus Spp

This study focuses on the evaluation of the microbiological profile of microbes found in water, soil, droppings, in selected poultry farms under intensive and semi-intensive management system within Lagos, Nigeria. Bacteria and fungi were isolated from poultry droppings, water and soil samples and identified by standard microbiological protocol. The data on the assessment of poultry production system were obtained with structured questionnaire. The bacterial and fungal counts ranged from 29 X 109 CFU/mL - 106 X 109 CFU/mL and 72 X 109 CFU/mL - 115 X 109 CFU/mL respectively. The microorganisms isolated were Streptococcus pluranimalium, Micrococcus sp., Pseudomonas aeruginosa, Salmonella sp., Staphylococcus arlettae, Escherichia coli, Staphylococcus saprophyticus, Bacilllus subtilis, Aeromonas hydrophila, Candida tropicalis, Saccharomyces spp, Sporendonema spp., Aspergillus fumigatus, Fusarium oxysporum, Kloeckera spp., Zygosaccharomyces spp and Aspergillus niger. Aspergillus niger (30.5%), Micrococcus sp. (17.6%) and Staphylococcus saprophyticus, (17.6%), occur most frequently while Candida tropicalis (4.6%) and Escherichia coli (5.8%) has the lowest percentage occurrence in poultry droppings. Aspergillus sp. (48.4%), Pseudomonas aeruginosa (28.6%) occur most frequently while Penicillium sp. (15.2%), Staphylococcus spp. (7.1%), has the lowest percentage occurrence in poultry soil samples. Fusarium sp. (38.8%) and Pseudomonas aeruginosa (28.6%), occur most frequently while Aspergillus sp. (7.7%), Staphylococcus spp. (7.1%) has the lowest percentage occurrence in water samples. Most of the isolated bacteria showed resistance to at least two different classes of antibiotics. There is strong evidence that poultry farms in Lagos have utilized many antibiotics and this may have contributed to antibiotic resistant pattern of some bacterial isolates to antibiotics.

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