scholarly journals Bio-treatment of soil from international airport in Ostrava

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Peter Fečko ◽  
Radmila Kučerová ◽  
Eva Pertile ◽  
Lucie Nezvalová ◽  
Nikolas Mucha ◽  
...  
Keyword(s):  
Pseudomonas Putida ◽  
Bacterial Culture ◽  
Laboratory Culture ◽  
Soil Samples ◽  
Mixed Bacterial Culture ◽  
International Airport ◽  
Pure Bacterial Culture ◽  
Rhodococcus Sp

The paper deals with an examination of possible application of biodegradation in the decontamination of soil samples from international airport in Ostrava. The laboratory biodegradation tests were carried out with a pure bacterial culture of Pseudomonas putida, a pure laboratory culture of Rhodococcus sp, their mixture and a mixture prepared combining their media free of bacteria. The results of the paper imply that for biodegradation of airport pollutants is most suitable to apply a mixed bacterial culture of Pseudomonas putida and Rhodococcus sp. The results show that the biodegradation method is applicable for the pollution.

scholarly journals Isolation of Mixed Bacterial Culture from Rajshahi Silk Industrial Zone and their Efficiency in Azo Dye Decolorization

2015 ◽  
Vol 8 (10) ◽  
pp. 950 ◽  
Author(s):  
Farhana Parvin ◽  
Muhammed Mizanur Rahman ◽  
Md. Mahmudul Islam ◽  
Naoshin Jahan ◽  
Md. Pallob Ebna Shaekh ◽  
...  
Keyword(s):  
Azo Dye ◽  
Bacterial Culture ◽  
Dye Decolorization ◽  
Industrial Zone ◽  
Mixed Bacterial Culture ◽  
Azo Dye Decolorization

scholarly journals The Platelet Fraction Is a Novel Reservoir to Detect Lyme Borrelia in Blood

Biology ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 366
Author(s):  
Victoria P. Sanderson ◽  
Iain L. Mainprize ◽  
Lisette Verzijlenberg ◽  
Cezar M. Khursigara ◽  
Melanie K. B. Wills
Keyword(s):  
Pathogen Detection ◽  
Bacterial Culture ◽  
Direct Detection ◽  
Laboratory Culture ◽  
Detection Methods ◽  
Clinical Samples ◽  
Blood Collection ◽  
Processing Conditions ◽  
Proof Of Concept ◽  
Clinical Detection

Serological diagnosis of Lyme disease suffers from considerable limitations. Yet, the technique cannot currently be replaced by direct detection methods, such as bacterial culture or molecular analysis, due to their inadequate sensitivity. The low bacterial burden in vasculature and lack of consensus around blood-based isolation of the causative pathogen, Borrelia burgdorferi, are central to this challenge. We therefore addressed methodological optimization of Borrelia recovery from blood, first by analyzing existing protocols, and then by using experimentally infected human blood to identify the processing conditions and fractions that increase Borrelia yield. In this proof-of-concept study, we now report two opportunities to improve recovery and detection of Borrelia from clinical samples. To enhance pathogen viability and cultivability during whole blood collection, citrate anticoagulant is superior to more commonly used EDTA. Despite the widespread reliance on serum and plasma as analytes, we found that the platelet fraction of blood concentrates Borrelia, providing an enriched resource for direct pathogen detection by microscopy, laboratory culture, Western blot, and PCR. The potential for platelets to serve as a reservoir for Borrelia and its diagnostic targets may transform direct clinical detection of this pathogen.

Rise and Fall of Bacterial Populations in Fish Muscle

1943 ◽  
Vol 6b (2) ◽  
pp. 119-128 ◽  
Author(s):  
H. L. A. Tarr
Keyword(s):  
Bacterial Culture ◽  
Bacterial Count ◽  
Fish Muscle ◽  
Growth Phases ◽  
Bacterial Populations ◽  
Count Method ◽  
Pure Bacterial Culture ◽  
Direct Counts

A direct bacterial count method applicable to fish muscle containing over approximately 1 × 105 bacteria per gram, with a modification employing preliminary incubation for muscle containing fewer bacteria, is described. Direct counts generally yielded similar and somewhat higher results than viable counts. Mixed bacterial populations of fish muscle generally exhibited the growth phases characteristic of a pure bacterial culture. There was absolutely no relation between direct (or viable) counts obtained subsequent to such incubation and the initial viable counts of the samples studied. Bacteria increased much more rapidly in excised fish muscle than in whole fish, and somewhat more rapidly in halibut than in salmon flesh.

scholarly journals Biodegradation of Methyl tert-Butyl Ether by a Pure Bacterial Culture

2001 ◽  
Vol 67 (12) ◽  
pp. 5601-5607 ◽  
Author(s):  
Paul B. Hatzinger ◽  
Kevin McClay ◽  
Simon Vainberg ◽  
Marina Tugusheva ◽  
Charles W. Condee ◽  
...  
Keyword(s):  
Bacterial Culture ◽  
Butyl Alcohol ◽  
The Other ◽  
Methyl Tert Butyl Ether ◽  
Butyl Ether ◽  
Pure Bacterial Culture ◽  
Degradation Activity ◽  
Type Strains ◽  
Mtbe Degradation ◽  
Allyl Thiourea

ABSTRACT Biodegradation of methyl tert-butyl ether (MTBE) by the hydrogen-oxidizing bacterium Hydrogenophaga flavaENV735 was evaluated. ENV735 grew slowly on MTBE ortert-butyl alcohol (TBA) as sole sources of carbon and energy, but growth on these substrates was greatly enhanced by the addition of a small amount of yeast extract. The addition of H2 did not enhance or diminish MTBE degradation by the strain, and MTBE was only poorly degraded or not degraded by type strains of Hydrogenophaga or hydrogen-oxidizing enrichment cultures, respectively. MTBE degradation activity was constitutively expressed in ENV735 and was not greatly affected by formaldehyde, carbon monoxide, allyl thiourea, or acetylene. MTBE degradation was inhibited by 1-amino benzotriazole and butadiene monoepoxide. TBA degradation was inducible by TBA and was inhibited by formaldehyde at concentrations of >0.24 mM and by acetylene but not by the other inhibitors tested. These results demonstrate that separate, independently regulated genes encode MTBE and TBA metabolism in ENV735.

Enrichment of a Mixed Bacterial Culture with a High Polyhydroxyalkanoate Storage Capacity

2009 ◽  
Vol 10 (4) ◽  
pp. 670-676 ◽  
Author(s):  
Katja Johnson ◽  
Yang Jiang ◽  
Robbert Kleerebezem ◽  
Gerard Muyzer ◽  
Mark C. M. van Loosdrecht
Keyword(s):  
Storage Capacity ◽  
Bacterial Culture ◽  
Mixed Bacterial Culture

Biological decolorization of industrial dyes by Candida tropicalis and Bacillus firmus

2011 ◽  
Vol 63 (4) ◽  
pp. 761-768 ◽  
Author(s):  
Sucharita Arora ◽  
Harvinder Singh Saini ◽  
Kamaljit Singh
Keyword(s):  
Candida Tropicalis ◽  
Bacterial Culture ◽  
Cellulose Triacetate ◽  
Domestic Sewage ◽  
Soil Samples ◽  
Environmental Concerns ◽  
Disperse Dyes ◽  
Yeast Culture ◽  
Bacillus Firmus ◽  
Textile Industries

Disperse dyes are chiefly used by textile industries for the coloration of polyester and cellulose triacetate and their blended fibres. Their extensive use and recalcitrant nature, high tinctorial strength renders the voluminous textile effluents intensively colored and causes environmental concerns. Decolorization of representative members of Dianix CC and Dianix S brands (DyStar Pvt. Ltd.) of disperse dyes were tested with Candida tropicalis and Bacillus firmus isolated respectively from contaminated soil samples and sludge of a domestic sewage drain. While both the cultures efficiently remove color from the aqueous solutions of the dyes, the yeast culture was found to decolorize most of the tested disperse dyes through biotransformation, the bacterial culture showed color removal mainly by adsorption on the cell pellets. Formation of cleavage products such as p-nitroaniline was observed in the case of Dianix Orange E-3R, indicating reductive cleavage of the azo linkage of the dye.

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