Identification by microarray of a common pattern of gene expression in intact intestine and cultured intestinal cells exposed to virulent Aeromonas hydrophila isolates

2006 ◽  
Vol 4 (3) ◽  
pp. 381-388 ◽  
Author(s):  
S. L. Hayes ◽  
B. R. Lye ◽  
D. J. Lye ◽  
M. R. Rodgers ◽  
G. Stelma ◽  
...  
Keyword(s):  
Gene Expression ◽  
Aeromonas Hydrophila ◽  
Intestinal Cells ◽  
Common Pattern ◽  
Virulent Aeromonas Hydrophila

scholarly journals Identification by microarray of a common pattern of gene expression in intact intestine and cultured intestinal cells exposed to virulent Aeromonas hydrophila isolates

2006 ◽  
Vol 4 (3) ◽  
pp. 381-388
Author(s):  
Samuel L. Hayes ◽  
Bethany R. Lye ◽  
Dennis J. Lye ◽  
Mark R. Rodgers ◽  
Gerard Stelma ◽  
...  
Keyword(s):  
Gene Expression ◽  
Aeromonas Hydrophila ◽  
Innate Immune ◽  
Intestinal Cell ◽  
Infection Model ◽  
Common Pattern ◽  
Intestinal Cell Line ◽  
Small Intestinal ◽  
Virulent Aeromonas Hydrophila ◽  
Common Infection

The genus Aeromonas comprises known virulent and avirulent isolates and has been implicated in waterborne disease. A common infection model of human gastroenteritis associated with A. hydrophila uses neonatal mice. The goal of this research was to evaluate whether a murine small intestinal cell line could provide comparable results to the gene expression changes in the neonatal mouse model. Changes in mRNA expression in host cell cultures and intestinal tissues were measured after exposure to virulent Aeromonas hydrophila strains. A. hydrophila caused the up-regulation of more than 200 genes in neonates and over 50 genes in cell culture. Twenty-six genes were found to be in common between the two models, of which the majority are associated with the innate immune response.

scholarly journals CFTR Cooperative Cis-Regulatory Elements in Intestinal Cells

2021 ◽  
Vol 22 (5) ◽  
pp. 2599
Author(s):  
Mégane Collobert ◽  
Ozvan Bocher ◽  
Anaïs Le Nabec ◽  
Emmanuelle Génin ◽  
Claude Férec ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cystic Fibrosis ◽  
Gene Regulation ◽  
Genetic Diseases ◽  
Regulatory Elements ◽  
Cftr Gene ◽  
Intestinal Cells ◽  
Cooperative Effects ◽  
Cis Acting ◽  
Study Techniques

About 8% of the human genome is covered with candidate cis-regulatory elements (cCREs). Disruptions of CREs, described as “cis-ruptions” have been identified as being involved in various genetic diseases. Thanks to the development of chromatin conformation study techniques, several long-range cystic fibrosis transmembrane conductance regulator (CFTR) regulatory elements were identified, but the regulatory mechanisms of the CFTR gene have yet to be fully elucidated. The aim of this work is to improve our knowledge of the CFTR gene regulation, and to identity factors that could impact the CFTR gene expression, and potentially account for the variability of the clinical presentation of cystic fibrosis as well as CFTR-related disorders. Here, we apply the robust GWAS3D score to determine which of the CFTR introns could be involved in gene regulation. This approach highlights four particular CFTR introns of interest. Using reporter gene constructs in intestinal cells, we show that two new introns display strong cooperative effects in intestinal cells. Chromatin immunoprecipitation analyses further demonstrate fixation of transcription factors network. These results provide new insights into our understanding of the CFTR gene regulation and allow us to suggest a 3D CFTR locus structure in intestinal cells. A better understand of regulation mechanisms of the CFTR gene could elucidate cases of patients where the phenotype is not yet explained by the genotype. This would thus help in better diagnosis and therefore better management. These cis-acting regions may be a therapeutic challenge that could lead to the development of specific molecules capable of modulating gene expression in the future.

scholarly journals Identification and characterization of virulent Aeromonas hydrophila Ah17 from infected Channa striata in river Cauvery and in vitro evaluation of shrimp chitosan

2020 ◽  
Vol 8 (2) ◽  
pp. 1272-1283 ◽  
Author(s):  
Vignesh Samayanpaulraj ◽  
Muthukumar Sivaramapillai ◽  
Sankara Naynar Palani ◽  
Krishnaveni Govindaraj ◽  
Vijay Velu ◽  
...  
Keyword(s):  
Aeromonas Hydrophila ◽  
In Vitro Evaluation ◽  
Channa Striata ◽  
Virulent Aeromonas Hydrophila ◽  
Identification And Characterization

Induction of apolipoprotein A-I gene expression by glucagon-like peptide-1 and exendin-4 in hepatocytes but not intestinal cells

Metabolism ◽  
2013 ◽  
Vol 62 (2) ◽  
pp. 265-274 ◽  
Author(s):  
Joe M. Chehade ◽  
Rosalyn Alcalde ◽  
Emad Naem ◽  
Arshag D. Mooradian ◽  
Norman C.W. Wong ◽  
...  
Keyword(s):  
Gene Expression ◽  
Intestinal Cells ◽  
Apolipoprotein A ◽  
Glucagon Like Peptide ◽  
Glucagon Like Peptide 1 ◽  
I Gene
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