Characterization of histopathological and ultrastructural changes in Myxocyprinus asiaticus acutely infected with virulent Aeromonas hydrophila

2021 ◽  
Author(s):  
Fang Li ◽  
Haoran Gu ◽  
Fang Ou ◽  
Hailong Ge ◽  
Xiaohong Liu ◽  
...  
Keyword(s):  
Aeromonas Hydrophila ◽  
Ultrastructural Changes ◽  
Myxocyprinus Asiaticus ◽  
Virulent Aeromonas Hydrophila

scholarly journals Identification and characterization of virulent Aeromonas hydrophila Ah17 from infected Channa striata in river Cauvery and in vitro evaluation of shrimp chitosan

2020 ◽  
Vol 8 (2) ◽  
pp. 1272-1283 ◽  
Author(s):  
Vignesh Samayanpaulraj ◽  
Muthukumar Sivaramapillai ◽  
Sankara Naynar Palani ◽  
Krishnaveni Govindaraj ◽  
Vijay Velu ◽  
...  
Keyword(s):  
Aeromonas Hydrophila ◽  
In Vitro Evaluation ◽  
Channa Striata ◽  
Virulent Aeromonas Hydrophila ◽  
Identification And Characterization

scholarly journals Isolation and characterization of bacteriophages against virulent Aeromonas hydrophila

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Jin Liu ◽  
Shanshan Gao ◽  
Yuhao Dong ◽  
Chengping Lu ◽  
Yongjie Liu
Keyword(s):  
Aeromonas Hydrophila ◽  
Isolation And Characterization ◽  
Virulent Aeromonas Hydrophila

scholarly journals Pathological findings of Chinese sucker (Myxocyprinus asiaticus) infected with virulent Aeromonas hydrophila

2021 ◽  
Vol 21 ◽  
pp. 100884
Author(s):  
Fang Li ◽  
Jianrong Zhao ◽  
Yueyue Zhao ◽  
Xiaohong Liu ◽  
Jing Huang ◽  
...  
Keyword(s):  
Aeromonas Hydrophila ◽  
Pathological Findings ◽  
Myxocyprinus Asiaticus ◽  
Chinese Sucker ◽  
Virulent Aeromonas Hydrophila

scholarly journals Molecular and Functional Characterization of a ToxR-Regulated Lipoprotein from a Clinical Isolate of Aeromonas hydrophila

2006 ◽  
Vol 74 (7) ◽  
pp. 3742-3755 ◽  
Author(s):  
Lakshmi Pillai ◽  
Jian Sha ◽  
Tatiana E. Erova ◽  
Amin A. Fadl ◽  
Bijay K. Khajanchi ◽  
...  
Keyword(s):  
Virulence Factor ◽  
Aeromonas Hydrophila ◽  
Functional Characterization ◽  
Affinity Column ◽  
Serum Resistance ◽  
Classical Pathway ◽  
Dependent Manner ◽  
T7 Promoter

ABSTRACT Human diseases caused by species of Aeromonas have been classified into two major groups: septicemia and gastroenteritis. In this study, we reported the molecular and functional characterization of a new virulence factor, ToxR-regulated lipoprotein, or TagA, from a diarrheal isolate, SSU, of Aeromonas hydrophila. The tagA gene of A. hydrophila exhibited 60% identity with that of a recently identified stcE gene from Escherichia coli O157:H7, which encoded a protein (StcE) that provided serum resistance to the bacterium and prevented erythrocyte lysis by controlling classical pathway of complement activation by cleaving the complement C1-esterase inhibitor (C1-INH). We purified A. hydrophila TagA as a histidine-tagged fusion protein (rTagA) from E. coli DE3 strain using a T7 promoter-based pET30 expression vector and nickel affinity column chromatography. rTagA cleaved C1-INH in a time-dependent manner. The tagA isogenic mutant of A. hydrophila, unlike its corresponding wild-type (WT) or the complemented strain, was unable to cleave C1-INH, which is required to potentiate the C1-INH-mediated lysis of host and bacterial cells. We indeed demonstrated colocalization of C1-INH and TagA on the bacterial surface by confocal fluorescence microscopy, which ultimately resulted in increased serum resistance of the WT bacterium. Likewise, we delineated the role of TagA in contributing to the enhanced ability of C1-INH to inhibit the classical complement-mediated lysis of erythrocytes. Importantly, we provided evidence that the tagA mutant was significantly less virulent in a mouse model of infection (60%) than the WT bacterium at two 50% lethal doses, which resulted in 100% mortality within 48 h. Taken together, our data provided new information on the role of TagA as a virulence factor in bacterial pathogenesis. This is the first report of TagA characterization from any species of Aeromonas.

scholarly journals Kinetic and spectroscopic characterization of native and metal-substituted β-lactamase from Aeromonas hydrophila AE036

FEBS Letters ◽  
2000 ◽  
Vol 467 (2-3) ◽  
pp. 221-225 ◽  
Author(s):  
Maria Hernandez Valladares ◽  
Martin Kiefer ◽  
Uwe Heinz ◽  
Raquel Paul Soto ◽  
Wolfram Meyer-Klaucke ◽  
...  
Keyword(s):  
Aeromonas Hydrophila ◽  
Spectroscopic Characterization
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