scholarly journals Identification by microarray of a common pattern of gene expression in intact intestine and cultured intestinal cells exposed to virulent Aeromonas hydrophila isolates

2006 ◽  
Vol 4 (3) ◽  
pp. 381-388
Author(s):  
Samuel L. Hayes ◽  
Bethany R. Lye ◽  
Dennis J. Lye ◽  
Mark R. Rodgers ◽  
Gerard Stelma ◽  
...  
Keyword(s):  
Gene Expression ◽  
Aeromonas Hydrophila ◽  
Innate Immune ◽  
Intestinal Cell ◽  
Infection Model ◽  
Common Pattern ◽  
Intestinal Cell Line ◽  
Small Intestinal ◽  
Virulent Aeromonas Hydrophila ◽  
Common Infection

The genus Aeromonas comprises known virulent and avirulent isolates and has been implicated in waterborne disease. A common infection model of human gastroenteritis associated with A. hydrophila uses neonatal mice. The goal of this research was to evaluate whether a murine small intestinal cell line could provide comparable results to the gene expression changes in the neonatal mouse model. Changes in mRNA expression in host cell cultures and intestinal tissues were measured after exposure to virulent Aeromonas hydrophila strains. A. hydrophila caused the up-regulation of more than 200 genes in neonates and over 50 genes in cell culture. Twenty-six genes were found to be in common between the two models, of which the majority are associated with the innate immune response.

Polyamines modulate phas-I gene expression in an intestinal cell line

1998 ◽  
Vol 114 ◽  
pp. A1144-A1145
Author(s):  
John Gill ◽  
Karin Suess ◽  
ER Seidel
Keyword(s):  
Gene Expression ◽  
Cell Line ◽  
Intestinal Cell ◽  
Intestinal Cell Line ◽  
I Gene

scholarly journals Traditional Herbal Medicine, Rikkunshito, Induces HSP60 and Enhances Cytoprotection of Small Intestinal Mucosal Cells as a Nontoxic Chaperone Inducer

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Kumiko Tamaki ◽  
Michiro Otaka ◽  
Tomoyoshi Shibuya ◽  
Naoto Sakamoto ◽  
Soh Yamamoto ◽  
...  
Keyword(s):  
Herbal Medicine ◽  
Immunoblot Analysis ◽  
Intestinal Cell ◽  
Traditional Herbal Medicine ◽  
Intestinal Cell Line ◽  
Mucosal Cells ◽  
Hsp60 Expression ◽  
Small Intestinal ◽  
Intestinal Ulcers

Increasing incidence of small intestinal ulcers associated with nonsteroidal anti-inflammatory drugs (NSAIDs) has become a topic with recent advances of endoscopic technology. However, the pathogenesis and therapy are not fully understood. The aim of this study is to examine the effect of Rikkunshito (TJ-43), a traditional herbal medicine, on expression of HSP60 and cytoprotective ability in small intestinal cell line (IEC-6). Effect of TJ-43 on HSP60 expression in IEC-6 cells was evaluated by immunoblot analysis. The effect of TJ-43 on cytoprotective abilities of IEC-6 cells against hydrogen peroxide or indomethacin was studied by MTT assay, LDH-release assay, caspase-8 activity, and TUNEL. HSP60 was significantly induced by TJ-43. Cell necrosis and apoptosis were significantly suppressed in IEC-6 cells pretreated by TJ-43 with overexpression of HSP60. Our results suggested that HSP60 induced by TJ-43 might play an important role in protecting small intestinal epithelial cells from apoptosis and necrosis in vitro.

Hepatocyte growth factor stimulates invasion across reconstituted basement membranes by a new human small intestinal cell line

1994 ◽  
Vol 12 (2) ◽  
pp. 143-154 ◽  
Author(s):  
Iruvanti Sunitha ◽  
Deborah L. Meighen ◽  
Dan -Paul Hartman ◽  
Erik W. Thompson ◽  
Stephen W. Byers ◽  
...  
Keyword(s):  
Growth Factor ◽  
Hepatocyte Growth Factor ◽  
Cell Line ◽  
Basement Membranes ◽  
Intestinal Cell ◽  
Intestinal Cell Line ◽  
Small Intestinal ◽  
Hepatocyte Growth

Differentiation of rat small intestinal epithelial cells by extracellular matrix

1988 ◽  
Vol 254 (3) ◽  
pp. G355-G360 ◽  
Author(s):  
K. M. Carroll ◽  
T. T. Wong ◽  
D. L. Drabik ◽  
E. B. Chang
Keyword(s):  
Extracellular Matrix ◽  
Basement Membrane ◽  
Glucose Absorption ◽  
Cell Maturation ◽  
Intestinal Cell ◽  
Synthetic Activity ◽  
Intestinal Epithelial ◽  
Intestinal Cell Line ◽  
Small Intestinal

The role of extracellular matrix as a determinant of intestinal cell maturation was explored by growing a normal, but immature, rat small intestinal cell line (IEC-6) on basement membrane extract from Engelbreth-Holm-Swarm (EHS) sarcoma cells (ECM). Grown on plastic or glass, these cells are relatively immature and proliferate rapidly. In contrast, cells on ECM attached more rapidly, stopped proliferating, and rapidly organized into multicellular complex structures. Ultrastructurally, cells grown on ECM displayed significantly more mitochondria, rough endoplasmic reticulum, apical microvilli, and complex golgi apparatus, consistent with greater maturity and synthetic activity. By indirect immunofluorescence, sucrase, alkaline phosphatase, and cellular apolipoprotein B were present in cells grown on ECM only. In contrast to cells grown on glass, these cells also demonstrated Na-dependent glucose absorption, a function unique to mature villus cells (7). We conclude that the basement membrane may be a key determinant of intestinal epithelial cell maturation. The development of a mature villuslike intestinal cell in vitro may have wide application for future studies of induction and regulation of intestinal maturation and function.

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