scholarly journals The Effect of some Insecticides on Two Entomopathogenic Nematodes and Evaluation of Its Virulence on the Greater Wax Moth Galleria mellonella

2017 ◽  
Vol 6 (1) ◽  
pp. 37-45 ◽  
Author(s):  
Dina Atwa ◽  
Mona Hussein ◽  
Mostafa El-Khawas ◽  
Awad Sarhan ◽  
Nasser Mandour ◽  
...  
Keyword(s):  
Entomopathogenic Nematodes ◽  
Galleria Mellonella ◽  
Greater Wax Moth ◽  
Wax Moth

Surface protein components from entomopathogenic nematodes and their symbiotic bacteria: effects on immune responses of the greater wax moth,Galleria mellonella(Lepidoptera: Pyralidae)

2018 ◽  
Vol 74 (9) ◽  
pp. 2089-2099 ◽  
Author(s):  
Maurizio Francesco Brivio ◽  
Andrea Toscano ◽  
Simone Maria De Pasquale ◽  
Andrea De Lerma Barbaro ◽  
Stefano Giovannardi ◽  
...  
Keyword(s):  
Immune Responses ◽  
Entomopathogenic Nematodes ◽  
Galleria Mellonella ◽  
Surface Protein ◽  
Symbiotic Bacteria ◽  
Greater Wax Moth ◽  
Lepidoptera Pyralidae ◽  
Protein Components ◽  
Wax Moth

scholarly journals THE CONTINUAL FORMING AND CONTRIBUTION OF INFECTIVE JUVENILES PRODUCED VIA ENDOTOKIA MATRICIDA OF ENTOMOPATHOGENIC NEMATODES IN THE FAMILY OF STEINERNEMATIDAE AND HETERORHABDITIDAE

2016 ◽  
Vol 10 (1) ◽  
pp. 26 ◽  
Author(s):  
Yuliantoro Baliadi ◽  
E. Kondo ◽  
T. Yoshiga
Keyword(s):  
Entomopathogenic Nematodes ◽  
Galleria Mellonella ◽  
Nematode Species ◽  
Total Production ◽  
Insect Host ◽  
Steinernema Glaseri ◽  
Greater Wax Moth ◽  
The Family ◽  
Insect Cadaver ◽  
Wax Moth

The non-feeding developmentally arrested infective juveniles (IJs) of entomopathogenic nematodes in the family of Steinernematidae and Heterorhabditidae seek out a susceptible insect host and initiate infections. The aim of the research was to examine the continual<br />forming and contribution of IJs produced via endotokia matricida (IJs-EM) of Heterorhabditis bacteriophora, Steinernema glaseri, and S. carpocapsae. The research was conducted at the Laboratory of Nematology of the Saga University, Japan (April 2001-April<br />2002) and the Laboratory of Nematology of the Indonesian Legume and Tuber Crops Research Institute (June 2003-October 2004). The nematode progenies were investigated using the greater wax moth, Galleria mellonella, pre-inoculated with 50 IJs at 25°C.<br />Results showed that three reproductive adult generations were observed at day 18th. There were 135,000, 128,000 and 133,000 IJs per insect cadaver produced in H. bacteriophora, S. glaseri and S. carpocapsae, respectively. Endotokia matricida contributed a higher number of IJs than that of a normal mode of IJs production. The ratios are 81%, 28% and 64% for H. bacteriophora, S. glaseri, and S. carpocapsae of the IJs total production, respectively. Among the generations, the highest contribution of IJs was come from the<br />third adult generation bearing endotokia matricida, i.e., 63%, 24% and 51% for the three nematode species. Although the IJs-EM were more transparent compared to the normal IJs, they were morphologically similar. The results show that endotokia matricida has a pivotal role in a species maintenance and survival strategy of entomopathogenic nematodes in extreme environmental conditions.

scholarly journals THE CONTINUAL FORMING AND CONTRIBUTION OF INFECTIVE JUVENILES PRODUCED VIA ENDOTOKIA MATRICIDA OF ENTOMOPATHOGENIC NEMATODES IN THE FAMILY OF STEINERNEMATIDAE AND HETERORHABDITIDAE

2016 ◽  
Vol 10 (1) ◽  
pp. 26 ◽  
Author(s):  
Yuliantoro Baliadi ◽  
E. Kondo ◽  
T. Yoshiga
Keyword(s):  
Entomopathogenic Nematodes ◽  
Galleria Mellonella ◽  
Nematode Species ◽  
Total Production ◽  
Insect Host ◽  
Steinernema Glaseri ◽  
Greater Wax Moth ◽  
The Family ◽  
Insect Cadaver ◽  
Wax Moth

The non-feeding developmentally arrested infective juveniles (IJs) of entomopathogenic nematodes in the family of Steinernematidae and Heterorhabditidae seek out a susceptible insect host and initiate infections. The aim of the research was to examine the continual<br />forming and contribution of IJs produced via endotokia matricida (IJs-EM) of Heterorhabditis bacteriophora, Steinernema glaseri, and S. carpocapsae. The research was conducted at the Laboratory of Nematology of the Saga University, Japan (April 2001-April<br />2002) and the Laboratory of Nematology of the Indonesian Legume and Tuber Crops Research Institute (June 2003-October 2004). The nematode progenies were investigated using the greater wax moth, Galleria mellonella, pre-inoculated with 50 IJs at 25°C.<br />Results showed that three reproductive adult generations were observed at day 18th. There were 135,000, 128,000 and 133,000 IJs per insect cadaver produced in H. bacteriophora, S. glaseri and S. carpocapsae, respectively. Endotokia matricida contributed a higher number of IJs than that of a normal mode of IJs production. The ratios are 81%, 28% and 64% for H. bacteriophora, S. glaseri, and S. carpocapsae of the IJs total production, respectively. Among the generations, the highest contribution of IJs was come from the<br />third adult generation bearing endotokia matricida, i.e., 63%, 24% and 51% for the three nematode species. Although the IJs-EM were more transparent compared to the normal IJs, they were morphologically similar. The results show that endotokia matricida has a pivotal role in a species maintenance and survival strategy of entomopathogenic nematodes in extreme environmental conditions.

scholarly journals Establishing an invertebrate Galleria mellonella greater wax moth larval model of Neisseria gonorrhoeae infection

Virulence ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 1900-1920
Author(s):  
Aiste Dijokaite ◽  
Maria Victoria Humbert ◽  
Emma Borkowski ◽  
Roberto M La Ragione ◽  
Myron Christodoulides
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Galleria Mellonella ◽  
Greater Wax Moth ◽  
Wax Moth

Transgenic expression of gallerimycin, a novel antifungal insect defensin from the greater wax moth Galleria mellonella, confers resistance to pathogenic fungi in tobacco

2006 ◽  
Vol 387 (5) ◽  
pp. 549-557 ◽  
Author(s):  
Gregor Langen ◽  
Jafargholi Imani ◽  
Boran Altincicek ◽  
Gernot Kieseritzky ◽  
Karl-Heinz Kogel ◽  
...  
Keyword(s):  
Transgenic Tobacco ◽  
Fungal Pathogens ◽  
Galleria Mellonella ◽  
Ectopic Expression ◽  
Pathogenic Fungi ◽  
Transgenic Expression ◽  
Greater Wax Moth ◽  
Insect Defensin ◽  
Wax Moth

Abstract A cDNA encoding gallerimycin, a novel antifungal peptide from the greater wax moth Galleria mellonella, was isolated from a cDNA library of genes expressed during innate immune response in the caterpillars. Upon ectopic expression of gallerimycin in tobacco, using Agrobacterium tumefaciens as a vector, gallerimycin conferred resistance to the fungal pathogens Erysiphe cichoracearum and Sclerotinia minor. Quantification of gallerimycin mRNA in transgenic tobacco by real-time PCR confirmed transgenic expression under control of the inducible mannopine synthase promoter. Leaf sap and intercellular washing fluid from transgenic tobacco inhibited in vitro germination and growth of the fungal pathogens, demonstrating that gallerimycin is secreted into intercellular spaces. The feasibility of the use of gallerimycin to counteract fungal diseases in crop plants is discussed.

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