scholarly journals Hemocyte Responses of the Colorado Potato Beetle,Leptinotarsa decemlineata, and the Greater Wax Moth,Galleria mellonella, to the Entomopathogenic Nematodes,Steinernema feltiaeandHeterorhabditis bacteriophora

2011 ◽  
Vol 11 (75) ◽  
pp. 1-13 ◽  
Author(s):  
L. Ebrahimi ◽  
G. Niknam ◽  
G. B. Dunphy
Keyword(s):  
Colorado Potato Beetle ◽  
Leptinotarsa Decemlineata ◽  
Entomopathogenic Nematodes ◽  
Galleria Mellonella ◽  
Potato Beetle ◽  
Greater Wax Moth ◽  
Wax Moth

scholarly journals Efficacy of the Applied Natural Enemies on the Survival of Colorado Potato Beetle Adults

Insects ◽  
2021 ◽  
Vol 12 (11) ◽  
pp. 1030
Author(s):  
Vladimír Půža ◽  
Jiří Nermuť ◽  
Jana Konopická ◽  
Oxana Skoková Habuštová
Keyword(s):  
Emerging Adults ◽  
Natural Enemies ◽  
Colorado Potato Beetle ◽  
Leptinotarsa Decemlineata ◽  
Entomopathogenic Fungus ◽  
Entomopathogenic Nematodes ◽  
Field Application ◽  
Fungal Treatment ◽  
Potato Plants ◽  
Potato Beetle

Colorado potato beetle Leptinotarsa decemlineata is among the most destructive pests of potatoes quickly developing resistance to traditional insecticides. In the present study, we tested the effect of various species and strains of entomopathogenic nematodes on CPB adults, and subsequently, the most effective nematodes were applied alone and in combination with entomopathogenic fungus B. bassiana in pots with potato plants and in the field and their effect on the number of emerging adults was evaluated. In the experimental infections, both the nematode invasion and pathogenicity were variable, and, in several strains, the mortality reached 100%. In pot experiments, soil application of nematodes S. carpocapsae 1343 and S. feltiae Jakub and fungus significantly decreased numbers of emerging CPB adults, while, after the application on leaves, only fungal treatment was effective. The field application of fungus B. bassiana significantly decreased the number of emerging CPB adults in comparison to control sites by ca. 30% while the effect of nematodes and the nematodes–fungus combination was not significant. In conclusion, we demonstrate the necessity of thorough bioassays to select the most effective nematode strains. Entomopathogenic nematodes have the potential to effectively decrease the emergence of CPB adults, but further research is needed to improve the effectiveness in the field.

Susceptibility of Prepupae of the Colorado Potato Beetle (Coleoptera: Chrysomelidae) to Entomopathogenic Nematodes (Rhabditida: Steinernematidae, Heterorhabditidae)

1992 ◽  
Vol 27 (1) ◽  
pp. 37-43 ◽  
Author(s):  
William W. Cantelo ◽  
William R. Nickle
Keyword(s):  
Colorado Potato Beetle ◽  
Leptinotarsa Decemlineata ◽  
Laboratory Tests ◽  
Entomopathogenic Nematodes ◽  
Parasitic Nematodes ◽  
Individual Treatment ◽  
Regression Equations ◽  
Potato Beetle ◽  
Leptinotarsa Decemlineata Say ◽  
The Cost

This research investigated the ability of five species or strains of parasitic nematodes to control the Colorado potato beetle, Leptinotarsa decemlineata (Say), when beetle prepupae were released on potting soil containing the nematodes. The nematodes tested were: Steinernema carpocapsae Weiser (All strain); S. carpocapsae (Mexican strain); S. feltiae (= bibionis) (Filipjev) (strain #27); S. feltiae (strain #980) and Heterorhabditis bacteriophora Poinar. Six dosage levels ranging from 10.3 to 329.2 nematodes/cm2, in addition to the water-only controls, were used in these laboratory tests. Each dosage-strain combination was replicated four times with 20 prepupae exposed to each individual treatment. The five strains demonstrated 100% kill at 164.6 nematodes/cm2. Except for the Mexican strain of S. carpocapsae, every strain produced over 98% mortality at 82.3 nematodes/cm2. Dosage response regression equations were calculated for each strain. Although field applications probably would require higher rates to obtain the same mortality, the use of parasitic nematodes has potential for suppressing Colorado potato beetle populations. The cost or dosage rate of nematodes may have to be reduced before the control costs would be acceptable to a grower.

Surface protein components from entomopathogenic nematodes and their symbiotic bacteria: effects on immune responses of the greater wax moth,Galleria mellonella(Lepidoptera: Pyralidae)

2018 ◽  
Vol 74 (9) ◽  
pp. 2089-2099 ◽  
Author(s):  
Maurizio Francesco Brivio ◽  
Andrea Toscano ◽  
Simone Maria De Pasquale ◽  
Andrea De Lerma Barbaro ◽  
Stefano Giovannardi ◽  
...  
Keyword(s):  
Immune Responses ◽  
Entomopathogenic Nematodes ◽  
Galleria Mellonella ◽  
Surface Protein ◽  
Symbiotic Bacteria ◽  
Greater Wax Moth ◽  
Lepidoptera Pyralidae ◽  
Protein Components ◽  
Wax Moth

scholarly journals THE CONTINUAL FORMING AND CONTRIBUTION OF INFECTIVE JUVENILES PRODUCED VIA ENDOTOKIA MATRICIDA OF ENTOMOPATHOGENIC NEMATODES IN THE FAMILY OF STEINERNEMATIDAE AND HETERORHABDITIDAE

2016 ◽  
Vol 10 (1) ◽  
pp. 26 ◽  
Author(s):  
Yuliantoro Baliadi ◽  
E. Kondo ◽  
T. Yoshiga
Keyword(s):  
Entomopathogenic Nematodes ◽  
Galleria Mellonella ◽  
Nematode Species ◽  
Total Production ◽  
Insect Host ◽  
Steinernema Glaseri ◽  
Greater Wax Moth ◽  
The Family ◽  
Insect Cadaver ◽  
Wax Moth

The non-feeding developmentally arrested infective juveniles (IJs) of entomopathogenic nematodes in the family of Steinernematidae and Heterorhabditidae seek out a susceptible insect host and initiate infections. The aim of the research was to examine the continual<br />forming and contribution of IJs produced via endotokia matricida (IJs-EM) of Heterorhabditis bacteriophora, Steinernema glaseri, and S. carpocapsae. The research was conducted at the Laboratory of Nematology of the Saga University, Japan (April 2001-April<br />2002) and the Laboratory of Nematology of the Indonesian Legume and Tuber Crops Research Institute (June 2003-October 2004). The nematode progenies were investigated using the greater wax moth, Galleria mellonella, pre-inoculated with 50 IJs at 25°C.<br />Results showed that three reproductive adult generations were observed at day 18th. There were 135,000, 128,000 and 133,000 IJs per insect cadaver produced in H. bacteriophora, S. glaseri and S. carpocapsae, respectively. Endotokia matricida contributed a higher number of IJs than that of a normal mode of IJs production. The ratios are 81%, 28% and 64% for H. bacteriophora, S. glaseri, and S. carpocapsae of the IJs total production, respectively. Among the generations, the highest contribution of IJs was come from the<br />third adult generation bearing endotokia matricida, i.e., 63%, 24% and 51% for the three nematode species. Although the IJs-EM were more transparent compared to the normal IJs, they were morphologically similar. The results show that endotokia matricida has a pivotal role in a species maintenance and survival strategy of entomopathogenic nematodes in extreme environmental conditions.

Tests of the Nematode DD 136 and an Associated Bacterium for Control of the Colorado Potato Beetle, Leptinotarsa decemlineata (Say)

1961 ◽  
Vol 93 (9) ◽  
pp. 759-763 ◽  
Author(s):  
H. E. Welch ◽  
L. J. Briand
Keyword(s):  
Colorado Potato Beetle ◽  
Leptinotarsa Decemlineata ◽  
Galleria Mellonella ◽  
Biological Control Agent ◽  
Infective Larva ◽  
Codling Moth ◽  
The Body ◽  
Potato Beetle ◽  
Large Numbers ◽  
The Dead

Extensive trials showed that a nematode of the family Neoaplectanidae and its associated bacterium did not act as a permanent or temporary biological control agent against the Colorado potato beetle, Leptinotarsa decemlineata (Say). These trials and the causes of the failure were investigated for a number of years at Belleville; the initial test was reported by Welch (1958). The nematode, commonly known as DD 136, was discovered in codling moth larvae by Dutky and Hough (1955). It is ingested with food by the host, passes through the gut wall into he haemocoele, and releases a bacterium that multiplies rapidly and kills the host. The nematode feelds on the body contents of the dead host, passes through several generations, and eventually leaves the host as an infective larva. Dutky, and we, found that many kinds of insects can be infected by the nematode in the laboratory. It may be reared in large numbers by infecting larvae of the greater wax moth, Galleria mellonella (L.). The larvae die within 36 hours at room temperature and are then placed on wet filter papers in a trap designed to recover the nematodes that emerge from the dead hosts. Storage of the nematodes in water at 45° F. for a month or more causes no decrease in vitality or infectivity, so that large numbers may be accumulated for field trials. These sturdy animals may be applied with a mechanical sprayer at pressures of 100 psi without injury.

Sign in / Sign up

Export Citation Format

Share Document