scholarly journals EFFECT OF LOW LEVEL LASER THERAPY ON ODONTOBLAST-LIKE CELLS DERIVED FROM HUMAN BONE MARROW MESENCHYMAL STEM CELLS (INVERTED PHASE CONTRAST LIGHT MICROSCOPE AND POLYMERASE CHAIN REACTION ASSESSMENT)

2016 ◽  
Vol 62 (2) ◽  
pp. 1417-1427
Author(s):  
Dina Hassouna ◽  
Rania Hassan ◽  
Dahlia Mohamed ◽  
Mostafa Gheith
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Polymerase Chain Reaction ◽  
Mesenchymal Stem Cells ◽  
Phase Contrast ◽  
Low Level Laser Therapy ◽  
Chain Reaction ◽  
Level Laser ◽  
Marrow Mesenchymal Stem Cells ◽  
Polymerase Chain

The Effect of the MicroRNA-183 Family on Hair Cell-Specific Markers of Human Bone Marrow-Derived Mesenchymal Stem Cells

2018 ◽  
Vol 23 (4) ◽  
pp. 208-215 ◽  
Author(s):  
Mohammad-Reza  Mahmoudian-Sani ◽  
Mohammad-Saeid Jami ◽  
Ali Mahdavinezhad ◽  
Razieh Amini ◽  
Gholamreza  Farnoosh ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Polymerase Chain Reaction ◽  
Mesenchymal Stem Cells ◽  
Hair Cell ◽  
Hair Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Chain Reaction ◽  
Polymerase Chain

Hearing loss is considered the most common sensory disorder across the world. Nowadays, a cochlear implant can be an effective treatment for patients. Moreover, it is often believed that sensorineural hearing loss in humans is caused by loss or disruption of the function of hair cells in the cochlea. In this respect, mesenchymal cells can be a good candidate for cell-based therapeutic approaches. To this end, the potential of human bone marrow-derived mesenchymal stem cells to differentiate into hair cells with the help of transfection of microRNA in vitro was investigated. MicroRNA mimics (miRNA-96, 182, and 183) were transfected to human bone marrow-derived mesenchymal stem cells using Lipofec­tamine as a common transfection reagent following the manufacturer’s instructions at 50 nM for microRNA mimics and 50 nM for the scramble. The changes in cell morphology were also observed under an inverted microscope. Then, the relative expression levels of SOX2, POU4F3, MYO7A, and calretinin were assayed using real-time polymerase chain reaction according to the ΔΔCt method. The ATOH1 level was similarly measured via real-time polymerase chain reaction and Western blotting. The results showed that increased expression of miRNA-182, but neither miRNA-96 nor miRNA-183, could lead to higher expression levels in some hair cell markers. The morphology of the cells also did not change in this respect, but the evaluation of gene expression at the levels of mRNA could promote the expression of the ATOH1, SOX2, and POU4F3 markers. Furthermore, miRNA-182 could enhance the expression of ATOH1 at the protein level. According to the results of this study, it was concluded that miRNA-182 could serve as a crucial function in hair cell differentiation by the upregulation of SOX2, POU4F3, and ATOH1 to promote a hair cell’s fate.

Differentiation of mesenchymal stem cells into neuron-like cells using composite 3D scaffold combined with valproic acid induction

2017 ◽  
Vol 32 (6) ◽  
pp. 702-715 ◽  
Author(s):  
Sadegh Ghorbani ◽  
Taki Tiraihi ◽  
Masoud Soleimani
Keyword(s):  
Stem Cells ◽  
Valproic Acid ◽  
Polymerase Chain Reaction ◽  
Mesenchymal Stem Cells ◽  
Lactic Acid ◽  
Reverse Transcription ◽  
Neural Regeneration ◽  
Poly Lactic Acid ◽  
Chain Reaction ◽  
Polymerase Chain

The nervous system has little capacity for self-repair after injury because neurons cannot proliferate owing to lack of suitable microenvironment. Therefore, neural tissue engineering that combines neural stem, scaffolds, and growth factors may improve the chance of restoration of damaged neural tissues. A favorable niche for neural regeneration would be both fibrous and electrically conductive scaffolds. Human Wharton jelly-derived mesenchymal stem cells were seeded on wet-electrospun 3D scaffolds composed of poly lactic acid coated with natural polymers including alginate and gelatin, followed by a multi-wall carbon nanotube coating. The results show that a wet-electrospun poly lactic acid scaffold at a concentration of 15% w/v had higher porosity (above 80%) than other concentrations. Moreover, the coated scaffold supported the growth of human Wharton jelly-derived mesenchymal stem cells in 3D culture, and were incubated for 21 days with 1 mM valproic acid as the inducer resulted in improvement in human Wharton jelly-derived mesenchymal stem cells differentiation into neuron-like cells immunoreactivity to nestin, Map2, and neuron specific enolase (NSE), which were also consistent with reverse transcription polymerase chain reaction (RT-PCR) and quantitive Reverse transcription polymerase chain reaction (qRT-PCR) results. The conclusion is that the 3D composite nanofiber poly lactic acid scaffold improved the transdifferentiation of human Wharton jelly-derived mesenchymal stem cells into neuron-like cells.

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