Optimization of Reference Genes for Normalization of Reverse Transcription Quantitative Real-Time Polymerase Chain Reaction Results in Senescence Study of Mesenchymal Stem Cells

2016 ◽  
Vol 25 (18) ◽  
pp. 1355-1365 ◽  
Author(s):  
Xiaodong Su ◽  
Xinglei Yao ◽  
Zhao Sun ◽  
Qin Han ◽  
Robert Chunhua Zhao
Keyword(s):  
Stem Cells ◽  
Polymerase Chain Reaction ◽  
Mesenchymal Stem Cells ◽  
Real Time ◽  
Reverse Transcription ◽  
Reference Genes ◽  
Chain Reaction ◽  
Polymerase Chain

Differentiation of mesenchymal stem cells into neuron-like cells using composite 3D scaffold combined with valproic acid induction

2017 ◽  
Vol 32 (6) ◽  
pp. 702-715 ◽  
Author(s):  
Sadegh Ghorbani ◽  
Taki Tiraihi ◽  
Masoud Soleimani
Keyword(s):  
Stem Cells ◽  
Valproic Acid ◽  
Polymerase Chain Reaction ◽  
Mesenchymal Stem Cells ◽  
Lactic Acid ◽  
Reverse Transcription ◽  
Neural Regeneration ◽  
Poly Lactic Acid ◽  
Chain Reaction ◽  
Polymerase Chain

The nervous system has little capacity for self-repair after injury because neurons cannot proliferate owing to lack of suitable microenvironment. Therefore, neural tissue engineering that combines neural stem, scaffolds, and growth factors may improve the chance of restoration of damaged neural tissues. A favorable niche for neural regeneration would be both fibrous and electrically conductive scaffolds. Human Wharton jelly-derived mesenchymal stem cells were seeded on wet-electrospun 3D scaffolds composed of poly lactic acid coated with natural polymers including alginate and gelatin, followed by a multi-wall carbon nanotube coating. The results show that a wet-electrospun poly lactic acid scaffold at a concentration of 15% w/v had higher porosity (above 80%) than other concentrations. Moreover, the coated scaffold supported the growth of human Wharton jelly-derived mesenchymal stem cells in 3D culture, and were incubated for 21 days with 1 mM valproic acid as the inducer resulted in improvement in human Wharton jelly-derived mesenchymal stem cells differentiation into neuron-like cells immunoreactivity to nestin, Map2, and neuron specific enolase (NSE), which were also consistent with reverse transcription polymerase chain reaction (RT-PCR) and quantitive Reverse transcription polymerase chain reaction (qRT-PCR) results. The conclusion is that the 3D composite nanofiber poly lactic acid scaffold improved the transdifferentiation of human Wharton jelly-derived mesenchymal stem cells into neuron-like cells.

1504: Molecular Diagnosis and Staging of Prostate Cancer Using Clusterin in Real Time Reverse Transcription Polymerase Chain Reaction (RT-PCR)

2006 ◽  
Vol 175 (4S) ◽  
pp. 485-486
Author(s):  
Sabarinath B. Nair ◽  
Christodoulos Pipinikas ◽  
Roger Kirby ◽  
Nick Carter ◽  
Christiane Fenske
Keyword(s):  
Prostate Cancer ◽  
Polymerase Chain Reaction ◽  
Real Time ◽  
Molecular Diagnosis ◽  
Reverse Transcription ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Polymerase Chain
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