scholarly journals Total Phenolic and Antioxidant Activity of an Aqueous Extract from Zizyphus Jujube Fruit from Çanakkale, Turkey

2019 ◽  
pp. 158-165
Author(s):  
Hasniye Yaşa
Keyword(s):  
Antioxidant Activity ◽  
Aqueous Extract ◽  
Total Phenolic ◽  
Jujube Fruit ◽  
Zizyphus Jujube

scholarly journals Antimicrobial and antioxidant activity of Epimedium pinnatum

2013 ◽  
Vol 59 (2) ◽  
pp. 24-34 ◽  
Author(s):  
Mohaddese Mahboubi ◽  
Nastaran Kazempour ◽  
Hossein Hosseini ◽  
Mona Mahboubi
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Aqueous Extract ◽  
Methanol Extract ◽  
Ethanolic Extract ◽  
Total Phenolic ◽  
Spectrophotometric Methods ◽  
Ic50 Value ◽  
Antioxidant And Antimicrobial Activity ◽  
Antimicrobial And Antioxidant Activity

Summary Epimedium pinnatum (Berberidaceae family) is used as an aphrodisiac in traditional Chinese medicine (TCM). The aim of this study was to evaluate the antimicrobial and antioxidant activity of E. pinnatum extracts (ethanol, methanol and aqueous extracts). Total phenolic (TPC) and flavonoid contents (TFC) of each extract were assessed by spectrophotometric methods. It was exhibited that methanol extract had better antimicrobial activity than those of ethanolic extract or aqueous extract. The TPC and TFC of E. pinnatum extracts was higher in methanol extract (149 and 36.6 mg/g) than that of ethanolic extract (137.2 and 19.5 mg/g) and aqueous extract (86.2 and 8.4 mg/g). The methanol extract had lower IC50 value (200 µg/ml) than ethanolic (250 µg/ml) and aqueous extract (400 µg/ml). There was a positive correlation between TPC, TFC in E. pinnatum extract and their antioxidant and antimicrobial activity

scholarly journals Total Phenolic Composition and Antioxidant Activity of Silver Nanoparticles using Aqueous Extract of Chilca Leaves (Baccharis latifolia)

2021 ◽  
Vol 18 (1) ◽  
pp. 79-91
Author(s):  
Fernanda Pilaquinga ◽  
Camila Granja ◽  
Eliza Jara ◽  
Mauricio Moncada-Basualto ◽  
Josu� Pozo-Mart�nez ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Silver Nanoparticles ◽  
Aqueous Extract ◽  
Total Phenolic ◽  
Phenolic Composition

A Comparative Study on Phytochemical Screening and Antioxidant Activity of Aqueous Extract from Various Parts of Moringa oleifera

2021 ◽  
Vol 3 (2) ◽  
pp. 43
Author(s):  
Awalul Fatiqin ◽  
Hanif Amrulloh ◽  
Ike Apriani ◽  
Aneke Lestari ◽  
Berta Erawanti ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Aqueous Extract ◽  
Total Phenolic Content ◽  
Moringa Oleifera ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Phytochemical Screening ◽  
Flavonoid Content ◽  
Leaf Part ◽  
Different Parts

In this work, we reported a comparative study on the phytochemical screening and antioxidant activity of aqueous extract from different parts of Moringa oleifera plant, i.e. leaves, twigs, stem barks, and woods. The phytochemical content of each extract was screened through qualitative analysis and followed by quantitative analysis for the determination of total phenolic content and total flavonoid content. The results showed that different parts of Moringa oleifera plant would give different extracted natural products. Either leaf or twig part of Moringa oleifera contained alkaloids, flavonoids, saponins, carbohydrates, polyphenols, proteins, amino acids, and phenolics. Meanwhile, only flavonoids, carbohydrates, and phenolics were found in the aqueous extract of the wood part of Moringa oleifera. In agreement to the phytochemical assay, the aqueous extract of leaf part of Moringa oleifera gave the highest total phenolic content (684 ± 16.7 µg GAE mL-1) and the highest flavonoid content (514 ± 26.1 µg CE mL-1) than other parts. Consequently, the leaf extract exhibited the highest antioxidant activity (64.6 ± 0.69%) which is close to the antioxidant activity of ascorbic acid (71.7 ± 0.56%) as the positive control. This finding is critical for the utilization of Moringa oleifera leaf part as the most potent source of antioxidant agents in the future.

Aqueous extract of andean berry induces apoptosis in human colon cancer cells without mitochondrial damage

2021 ◽  
pp. 1-16
Author(s):  
Sandra S. Arango-Varela ◽  
David Torres-Camargo ◽  
Camilo Reyes-Dieck ◽  
Maria Bibiana Zapata-Londoño ◽  
Maria E. Maldonado-Celis
Keyword(s):  
Cell Cycle ◽  
Antioxidant Activity ◽  
Cell Cycle Arrest ◽  
Aqueous Extract ◽  
Human Colon ◽  
Mitochondrial Damage ◽  
Antiproliferative Effect ◽  
Total Phenolic ◽  
Cycle Arrest ◽  
Sw620 Cells

BACKGROUND: Andean berry contains several classes of phenolic compounds which have showed antioxidant and cytotoxic activity. OBJECTIVE: To characterize the Andean berry aqueous extract and to study their anti-proliferative mechanisms on SW480 and SW620 cell lines (human colon adenocarcinoma). METHODS: Total phenolic and total anthocyanins contents were determined by Folin-Ciocalteau and differential pH methods, respectively. Antioxidant activity was measured by FRAP, ORAC and DPPH methods. Antiproliferative effect was determined by sulforhodamine colorimetric method and apoptosis was analyzed by flow cytometry using propidium iodide/Annexin-V. Mitochondrial potential was evaluated using DIOC6 and ROS levels were measured by 2,7-Dicholorodihydrofluorescein diacetate (DCFH-DA). RESULTS: The total phenol and anthocyanin content were 4409.78± 63,05 mg equivalents of gallic acid/100 mL and 106,57± 1.43 mg equivalents of cyanidin-3-glycoside/100 mL, respectively. Andean berry extract showed antioxidant activity by FRAP, ORAC and DPPH methods and antiproliferative effect on SW480 and SW620 cells. It was observed a cell cycle arrest at S and G2/M phases on SW480 and at G0/G1 phase on SW620 cells. Aqueous extract did not induce mitochondrial depolarization or affect intracellular ROS levels. CONCLUSIONS: Andean berry aqueous extract has antioxidant capacity and induces apoptosis involving cell cycle arrest in SW480 and SW620 cells without mitochondrial damage.

scholarly journals Toxicological properties of an aqueous extract of Aristolochia triangularis leaves, using the brine shrimp lethality and Allium cepa bioassays

2019 ◽  
Vol 49 (8) ◽  
Author(s):  
José Darlan Alves da Silva ◽  
Cláudio Rodrigo Nogueira ◽  
Maria do Carmo Vieira ◽  
Silvia Cristina Heredia-Vieira ◽  
Alexeia Barufatti ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Allium Cepa ◽  
Aqueous Extract ◽  
Brine Shrimp ◽  
Phenolic Content ◽  
Artemia Salina ◽  
Total Phenolic ◽  
Antioxidant Power ◽  
Ferric Reducing Antioxidant Power ◽  
Beneficial Property

ABSTRACT: The aqueous extract of Aristolochia triangularis leaves showed activity against Artemia salina larvae, with an LD50 of 370.6 µg/mL. In experiments with Allium cepa seeds, the extract caused a 51.26% reduction in the germination index, inhibited mean root growth, and was cytotoxic at concentrations of 668 and 2,000 µg/mL. Its antioxidant activity was additionally assessed in this research and the ferric reducing antioxidant power value was found to be 391.2 µM/g. Such health-beneficial property can be attributed partly to the total phenolic content, spectrophotometrically determined as 52.67 mg/g. The occurrence of cytotoxicity suggested caution when consuming teas from A. triangularis leaves for medicinal purposes, and equally reveals the need for further studies to investigate their adverse effects.

scholarly journals Total Phenolic Contents, DPPH Radical Scavenging and β-Carotene Bleaching Activities of Aqueous Extract from Ammoides atlantica

2020 ◽  
Vol 10 (3-s) ◽  
pp. 196-198
Author(s):  
Karima Loucif ◽  
Hassiba Benabdallah ◽  
Fatima Benchikh ◽  
Soulaf Mehlous ◽  
Chawki Ben Souici ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Medicinal Plants ◽  
Aqueous Extract ◽  
Radical Scavenging ◽  
Health Benefit ◽  
Total Phenolic ◽  
Dpph Radical ◽  
Dpph Radical Scavenging ◽  
Β Carotene

Phytotherapy has known a great evolution all the world and some medicinal plants are important remedies of some diseases. Ammoides atlantica is one of the medicinal plants used in folk medicine. This study aims to estimate the total phenolics and flavonoids contents then to investigate both in vitro antioxidant activity models of aqueous extract (AqE) from Ammoides atlantica. Total polyphenol contents were determined using Folin Ciocalteu's reagent; flavonoids were quantified employing the AlCl3 Method. The in vitro antioxidant property was assessed by DPPH-scavenging radicals and lipid peroxidation assays. The results revealed that Ammoides atlantica aqueous extract presented a high total phenolic and flavonoid contents with values of 85.56±4.71 µg GAE (gallic acid equivalent)/mg and 40.55±4.09 µg QE (quercitin equivalent)/mg dry extract, respectively. This extract shows a good DPPH radical scavenging and β-carotene bleaching activities with an IC50 of 107.48±5.9 µg/mL and 130.17±5.52 µg/mL, respectively. This study indicates that the aqueous extract from Ammoides atlantica has potent antioxidant effects and may prove to be of latent health benefit as well as supplementary sources for natural antioxidants drugs. Keywords: Ammoides atlantica, aqueous extract, antioxidant activity, phenolic compounds.

scholarly journals The impact of Senjed (Elaeagnus angustifolia L.) peel aqueous extract on qualitative properties of cold-pressed sesame oil

2021 ◽  
Author(s):  
Isa Fathollahy
Keyword(s):  
Antioxidant Activity ◽  
Aqueous Extract ◽  
Stability Index ◽  
Extraction Yield ◽  
Total Phenolic ◽  
Qualitative Properties ◽  
Oxidative Stability Index ◽  
Cold Pressed ◽  
Storage Times ◽  
The Impact

The Senjed peel aqueous extract was added to sesame cold-pressed oil at different concentration of 0, 100, 200, 500 and 750 mg/kg and compared with TBHQ (200 mg/kg). The Senjed peel aqueous extract showed 9.55 ± 0.35% extraction yield, 58.17 ± 2.33% antioxidant activity, 573.31 ± 3.57 (mg GAE/100 g FW) total phenolic content, and 151.33 ± 2.67 (mg CAE/100 g FW) total flavonoid content. The extract preserved 59.08% of antioxidant activity after heating at 185 ℃ for 80 min. Increasing the concentration of the extract caused a significant (p<0.05) qualitative improvement in the oil samples. At all storage times, the highest FFA%, peroxide and P-anisidine value belonged to oil samples containing 0 ppm of Senjed peel aqueous extract and followed by 100, 200, 500 and 750 ppm, respectively. The highest oxidative stability index was observed in oil samples containing TBHQ and 750 mg/kg concentration of extract (both 15.39 h). Oil samples containing 750 ppm of Senjed peel aqueous extract and TBHQ showed almost similar qualitative characteristics. Results showed that Senjed peel aqueous extract have antioxidant potential for stabilization of oils.

scholarly journals Phytochemical, Antioxidant and Anti-Inflammatory Effects of Extracts from Ampelocissus africana (Lour) Merr (Vitaceae) Rhizomes

2020 ◽  
pp. 8-18
Author(s):  
W. Leila Marie Esther Belem- Kabré ◽  
Noufou Ouédraogo ◽  
Adjaratou Compaoré- Coulibaly ◽  
Mariam Nebié- Traoré ◽  
Tata K. Traoré ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Aqueous Extract ◽  
Methanolic Extract ◽  
Total Phenolic ◽  
Antioxidant Power ◽  
Cox 2 ◽  
Anti Inflammatory ◽  
Cox 1

Aims: To determine the phytochemical composition and evaluate the antioxidant and anti-inflammatory properties of methanolic and aqueous extracts from Ampelocissus africana (Lour) Merr rhizomes. Study Design: Phytochemical content and screening, in vitro antioxidant and anti-inflammatory assays. Place and Duration of Study: Department of Traditional Pharmacopoeia and Pharmacy (MEPHATRA / PH) of the Institute for Research in Health Sciences (IRSS) and Laboratory of Applied Biochemistry and Chemistry (LABIOCA), University Joseph KI-ZERBO in Ouagadougou between April and August 2020. Methodology: The antioxidant activity of both extracts of the plant was assessed using DPPH radical scavenging, ABTS+ radical cation decolorization, ferric ion reduction and lipid peroxidation inhibition in rat liver assays. The anti-inflammatory activities in vitro were measured on the ability of the extract to inhibit the activity of enzymes such as 15-lipoxygenase, phospholipase A2 (PLA2) and cyclooxygenases (COX-1 and COX-2). Results: This study revealed that the total phenolic contents of the extracts varied from 471.79 ± 1.71 mg GAE/g to 173.88 ± 1.71 mg GAE/g for methanolic and aqueous extract respectively. The extracts were also rich in flavonoids and tannins. The methanolic extract possessed better antioxidant activity with an IC50 of 2.32 ± 0.18 µg/ml for the ABTS, 1.71 ± 0.05 µg/mL for the DPPH, a reducing power agent of 87.44 ± 0.5 mmol AAE /100 g and a percentage inhibition of lipid peroxidation of 52.21%. The methanolic and aqueous extract of A. africana has an inhibitory action on activity of lipoxygenase with IC50 values of 26.09 ± 1.83 μg/mL to 34.32 ± 1.60 μg/mL, respectively. The methanolic extract caused COX-1 inhibition of 36.07%, COX-2 inhibition of 38.31% and PLA2 inhibition of 26.9%. Conclusion: These results showed that the methanolic and aqueous extract from the rhizomes of Ampelocissus africana possessed antioxidant power, inhibitor effect against proinflammatory enzymes.

scholarly journals Total Phenol and Antioxidant Activity of Ethanol Extract and Water Extract from Claw Uncariaa gambir Roxb

2021 ◽  
Vol 9 (3) ◽  
pp. 131
Author(s):  
Lucky Hartanti ◽  
Asri Mulya Ashari ◽  
Warsidah Warsidah
Keyword(s):  
Antioxidant Activity ◽  
Aqueous Extract ◽  
Water Extract ◽  
Ethanol Extract ◽  
Total Phenol ◽  
Total Phenolic ◽  
Market Demand ◽  
University Campus ◽  
Industrial Plants

Uncaria gambier Roxb is a plant from the Rubiaceae family, belongs to the Bajakah group and has been used empirically in the treatment of various types of diseases in the community. Besides being one of the industrial plants and export commodities produced in Sumatra and Kalimantan, gambier leaves is also used frequently in Indonesia, both for the pharmaceutical and cosmetic industries. As a result, market demand both nationally and internationally have increased. The aim of this research are determination of antioxidant activity of ethanol extract and aqueous extract of gambir claw using 1,1-diphenil-2-picrylhydrazyl (DPPH) and determination of total phenol using Calcetau folin reagent. Gambier plant samples were taken in the forest area around the Tanjungpura University campus, the claws were separated and dried at room temperature, then powdered and extracted by maceration using 70% ethanol pa and aquadest, then the extract was concentrated until it was ready to be tested for antioxidant activity and determination of total phenol. The total phenolic ethanol extract and aqueous extract of gambir claw were 224.66 mg GAE/g extract (GEA : Gallic Acid Eqivalent) and 299.08 mg GAE/g extract, respectively, while the antioxidant potential (IC50) was 39.566 μg/mL and 65.140 μg/mL, and the IC50 for comparison of Vitamin C was 7.02 μg/mL.

scholarly journals Bioactive Compounds and Antioxidant Properties of Banana Inflorescence in a Beverage for Maternal Breastfeeding

2020 ◽  
Vol 11 (1) ◽  
pp. 343
Author(s):  
Doungporn Amornlerdpison ◽  
Vachira Choommongkol ◽  
Kanjana Narkprasom ◽  
Susanha Yimyam
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Bioactive Compounds ◽  
Aqueous Extract ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Microbial Pathogens ◽  
Total Phenolic ◽  
Total Phenolic Compounds ◽  
Health Food

Banana inflorescence is consumed as a traditional Thai cuisine for milk lactation in maternal breastfeeding. In this study, the inflorescence of banana (Musa x paradisiaca) was extracted in various solvents to determine the bioactive compounds and antioxidant activity in 2,2′-azino-bis 3-ethylbenzthiazoline-6-sulfonic acid (ABTS) radical scavenging. A suitable extract was developed into a beverage. We compared the results for the amount of total phenolic compounds and the capability of antioxidants obtained in polar and non-polar solvents. The extract in a high-polarity solvent demonstrated high total phenolic compounds and flavonoids. The bioactive compounds of banana inflorescence contained β- sitosterol, flavonoids, saponin, and other phenolic compounds such as catechin and isoquercetin. The aqueous extract of banana inflorescence was developed to act as a primary beverage ingredient. The beverage containing the aqueous extract of banana inflorescence (BAB) exhibited a brownish-yellow color and displayed high acidity and high total phenolic compounds, which are responsible for the antioxidant activity. The food processing of BAB showed no contamination of microbial pathogens. From our results, we concluded that banana inflorescence is a beneficial health food supplement for general consumers. Additionally, the beverage provides convenience and an alternative drink for postpartum mothers who breastfeed for their infants.

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