THE USE OF RAPD-PCR AND PCR-RFLP MOLECULAR MARKERS TO GENETICALLY DISTINGUISH THE MORPHOLOGICALLY CLOSE TWO SPECIES OF METAPENAEUS GENUS FROM TWO DIFFERENT ENVIRONMENTAL LOCATIONS( KAUR ABDULLAH & SHATT AL ARAB ) IN WATERS SOUTHERN OF IRAQ.

2018 ◽  
Vol 6 (4) ◽  
pp. 505-516
Author(s):  
Rabeehamankhi jebura ◽  
◽  
Israaadil fadhil ◽  
Keyword(s):  
Molecular Markers ◽  
Rapd Pcr ◽  
Pcr Rflp

Molecular typing of Staphylococcus aureus based on PCR-RFLP of coa gene and RAPD analysis

2011 ◽  
Vol 14 (2) ◽  
pp. 285-286 ◽  
Author(s):  
J. Karakulska ◽  
A. Pobucewicz ◽  
P. Nawrotek ◽  
M. Muszyńska ◽  
A. Furowicz ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Species Identification ◽  
Genetic Relationship ◽  
Molecular Typing ◽  
Rapd Analysis ◽  
Milk Samples ◽  
Rapd Pcr ◽  
Pcr Rflp ◽  
Mastitic Milk ◽  
Coa Gene

Molecular typing ofStaphylococcus aureusbased on PCR-RFLP ofcoagene and RAPD analysisThe aim of this study was molecular identification ofS. aureusstrains isolated from mastitic milk samples and establishing the genetic relationship between strains isolated from cows belonging to the same herd. In all 43 isolated strains thegapgene (930 bp) was amplified, which enabled their affiliation to theStaphylococcusgenus to be established. PCR-RFLP withAluI endonuclease of thegapgene as well asnuc(450 bp) andcoa(1130 bp) gene amplification allowed preciseS. aureusspecies identification. One hundred percent of the genetic relationship between strains was establishedviaRAPD-PCR and coa-typing.

Identifying chironomids (Diptera: Chironomidae) for biological monitoring with PCR–RFLP

2003 ◽  
Vol 93 (6) ◽  
pp. 483-490 ◽  
Author(s):  
M.E. Carew ◽  
V. Pettigrove ◽  
A.A. Hoffmann
Keyword(s):  
Molecular Markers ◽  
Large Scale ◽  
Life Stage ◽  
Biological Indicators ◽  
Geographic Area ◽  
Common Species ◽  
Rapid Identification ◽  
Chironomid Species ◽  
Pcr Rflp ◽  
Identification Of Species

AbstractChironomids are excellent biological indicators for the health of aquatic ecosystems, but their use at finer taxonomic levels is hindered by morphological similarity of species at each life stage. Molecular markers have the potential to overcome these problems by facilitating species identification particularly in large-scale surveys. In this study, the potential of the polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) approach was tested to rapidly distinguish among chironomids within a geographic area, by considering chironomid species from Melbourne, Australia. By comparing molecular markers with diagnostic morphological traits, RFLP profiles of the cytochrome oxidase I (COI) region were identified that were specific to genera and some common species. These profiles were used to develop an RFLP–based key, which was validated by testing the markers on samples from several wetlands and streams. As well as allowing for rapid identification of species that are difficult to separate on morphological grounds, this approach also has the potential to resolve current taxonomic ambiguities.

scholarly journals Selection of powdery mildew resistant and susceptible grapevine genotypes with molecular markers

2007 ◽  
pp. 100-104
Author(s):  
Stella Molnár ◽  
Zsuzsanna Galbács ◽  
Gábor Halász ◽  
Sarolta Hoffmann ◽  
Anikó Veres ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Powdery Mildew ◽  
Marker Assisted Selection ◽  
Powdery Mildew Resistance ◽  
Resistance Breeding ◽  
Breeding Programs ◽  
Pcr Rflp ◽  
Muscadinia Rotundifolia ◽  
Selection Of ◽  
Grape Breeding

Incorporation of competitive quality and resistance against the most important fungal diseases (powdery and downy mildew) in a cultivar is one of the most important aims of grapevine breeding. In the 20th century, the most advanced results in grapevine resistance breeding were achieved by French researchers. They used resistant cultivars in more than 30% of their growing areas. In these varieties, North American wild Vitisspecies were the resistance gene sources. The discovery of immunity-like resistance of Muscadinia rotundifolia opened new perspectives in resistance breeding. M. rotundifolia harbours a dominant powdery mildew gene, providing resistance in highquality cultivars after back-crosses with V. vinifera varieties. M. rotundifolia has been involved in the Hungarian grape breeding programs since 1996, thanks to a French-Hungarian variety exchange. In addition to traditional selection methods, application of MAS (Marker Assisted Selection) based on various types ofmolecular markers, can provide additional tools for these efforts. Run1 locus, responsible for powdery mildew resistance, was identified in Muscadinia rotundifolia. Molecular markers closely linked to this locus are very significant in screening progenies deriving from M. rotundifolia and V. vinifera crosses, making possible the discrimination between resistant and susceptible genotypes at DNA level. In our analyses BC5 progeny of {(M. rotundifola×V. vinifera) BC4}×Cardinal (V. vinifera) tested for powdery symptoms were analysed with PCR-RFLP (GLP1- 12P1P3) and microsatellite markers (VMC4f3.1, VMC8g9). Our results proved the applicability of the linked markers and reliability of marker assisted selection.

scholarly journals Genetic Diversity Assessment of Luffa aegyptiaca Landraces Endemic in Egypt Based on Some Molecular Markers

2016 ◽  
Vol 26 (2) ◽  
pp. 209-217
Author(s):  
Mohammad Abdel Sttar Al Tahlawy ◽  
Mahmoud Abdel Aziz Ibrahim ◽  
Mohamed Ahmed Matter ◽  
Mervat El Sayed Mohamed ◽  
Mahmoud Mohamed Sakr
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Total Proteins ◽  
Pcr Marker ◽  
Polymorphic Loci ◽  
Average Percentage ◽  
Rapd Pcr ◽  
Diversity Assessment ◽  
Sds Page ◽  
Luffa Aegyptiaca

Luffa aegyptiaca is a popular climbing herb endemic in Egypt. We studied the genetic diversity among ten Luffa landraces (Cairo, Beni Suef, Menoufiya, Damietta, Banha, Aswan, Kafr el?Sheikh, Bir el?Abd, MarsaMatruh and Asyut) collected from different districts in Egypt. The results obtained from DNA fingerprinting revealed that there were polymorphic loci with average percentage of 44.6 among collected landraces whereas polymorphic loci obtained from SDS?PAGE were 23%. Discrimination between landraces was more efficient by using RAPD?PCR marker than total proteins SDS?PAGE which showed a limited level of intraspecific diversity.Plant Tissue Cult. & Biotech. 26(2): 209-217, 2016 (December)

The development of codominant PCR/RFLP based markers for the flax rust-resistance alleles at the L locus

Genome ◽  
1999 ◽  
Vol 42 (1) ◽  
pp. 1-8 ◽  
Author(s):  
G Hausner ◽  
K Y Rashid ◽  
E O Kenaschuk ◽  
J D Procunier
Keyword(s):  
Molecular Markers ◽  
Nucleotide Sequence ◽  
Marker Assisted Selection ◽  
Rust Resistance ◽  
Single Gene ◽  
Pcr Markers ◽  
Genetic Studies ◽  
New Genes ◽  
Pcr Rflp ◽  
Primer Sets

The flax L locus exists as a single gene with at least 13 alleles with different rust-resistance specificities. With regards to resistance to North American races of flax rust the L2, L6, and L11 alleles are of major importance. Molecular markers have been developed by screening primer sets, whose sequences were based on the nucleotide sequence of L6, for their ability to amplify segments of the L gene. One primer combination was found to amplify only the L6 or L11 alleles and another primer set was found to amplify the 3' end of all important L alleles. The latter primer set yielded a 1.3 kb fragment which upon digestion with the endonuclease MboI generated RFLP patterns unique to L2, L6, L9, and L11. The application of PCR markers to a set of 22 cultivars, comprised of deregistered, recent, and yet to be released cultivars verifies genetic studies done by previous workers and demonstrates the usefulness of the markers for following segregation of L alleles in crosses amongst wide or narrow selections of cultivars. Overall, the results confirmed that L6 is present in many Canadian flax cultivars. However, in several recently-released flax cultivars that have rust resistance conditioned by genes at other loci, the L9 allele was detected. These molecular markers will be useful in marker-assisted selection and the introduction of new genes for rust resistance in the flax breeding programs.Key words: flax rust, PCR/RFLP marker, marker-assisted selection.

Discriminatory Power of RAPD,PCR-RFLP and Southern Blot Analyses of ureCD or ureA Gene Probes on Helicobacter pylori Isolates

2002 ◽  
Vol 57 (5-6) ◽  
pp. 516-521 ◽  
Author(s):  
Stella Smith ◽  
Franck Cantet ◽  
Fabrice Angelini ◽  
Armelle Marais ◽  
Francis Mégraud ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Helicobacter Pylori ◽  
Southern Blot ◽  
Blot Analysis ◽  
Southern Blot Analysis ◽  
Discriminatory Power ◽  
Gene Probes ◽  
Rapd Pcr ◽  
Pcr Rflp

The genetic diversity of 33 Nigerian Helicobacter pylori isolates were studied using RAPD, PCR-RFLP and Southern blot analysis of ureA or ureCD gene probes. RAPD was able to distinguish the following number of isolates using the primers 3880 : 5ʹ-AAGAGCCCGT-3ʹ (28), 3881 :5ʹ-AACGCGCAAC-3ʹ (33) and OPH8 :5ʹ-GAAACACCCC-3ʹ (25). Southern blot analysis using the ureCD probe was also able to distinguish the 12 isolates tested into ten different patterns. The PCR-RFLP technique distinguished all 33 isolates into six types. In conclusion, considering typeability, discriminatory power, and convenience, RAPD with the 3881 primer was considered the most useful technique.

scholarly journals Дослідження впливу складу захисного середовища на збереження життєздатності ліофілізованих бактерій L. lactis та L. plantarum, виділених із традиційної карпатської бринзи

2017 ◽  
Vol 19 (75) ◽  
pp. 29-34
Author(s):  
O.I. Tsisaryk ◽  
I.M. Slyvka ◽  
L.Ia. Musiy
Keyword(s):  
Rapd Pcr ◽  
Pcr Rflp

Вивчено вплив складу захисних середовищ на збереження життєздатності та технологічні властивості ліофілізованих бактерій штамів L. lactis та L. plantarum. Для досліджень обрано білково-вуглеводне та сахарозо-желатозне середовища. Об’єктами досліджень були чисті культури молочнокислих бактерій (МКБ): L. lactis SB 16, L. lactis SB 44, L. plantarum SB 5, L. plantarum SB 7, L. plantarum SB 17, виділені на попередніх етапах роботи із традиційної бринзи, виготовленої у непромислових умовах Карпатського регіону України. Культури бактерій ідентифіковано із використанням класичних мікробіологічних і сучасних молекулярно-генетичних методів (RAPD-PCR, RFLP-PCR, секвенування гену 16S рРНК). Чисельність мезо- і термофільних лактобактерій визначали шляхом посіву на середовище MRS. Молокозсідальну активність монокультур визначали за зміною кислотності молока. Процес сублімаційного сушіння біомаси, змішаної із відповідною кількістю захисного середовища, здійснювали із використанням сублімаційної сушарки «Alpha 1,2 LD Plus». За визначеною експериментальним шляхом кількістю загиблих під час сушіння клітин встановлювали Рівень Збереження Життєздатності (РЗЖ) різних видів і штамів. Встановлено, що за своїми кріопротекторними властивостями кращим для сушіння клітин досліджених монокультур є сахарозо-желатозне середовище, яке забезпечує високий РЗЖ, особливо для L. lactis SB 16 (кількість загиблих під час сушіння клітин становила 9,2%) та L. plantarum SB 17 (кількість загиблих під час сушіння клітин становила 9,9%). Проведено порівняльне дослідження РЗЖ різних видів МКБ за різних умов зберігання. Встановлено, що усі штами молочнокислих бактерій, за винятком штаму L. lactis SB 16, протягом зберігання у знежиреному молоці за температури (+6) °С через місяць втрачають від 4 до 15% життєздатних клітин. Термін зберігання штамів L. lactis та L. plantarum у сухій культурі становить 6 міс.

scholarly journals Pengelompokan dan Struktur Populasi Parasitoid Telur Trichogrammatoidea armigera pada Telur Helicoverpa armigera pada Jagung Berdasarkan Karakter Molekuler

2015 ◽  
Vol 7 (1) ◽  
pp. 54
Author(s):  
BAHAGIAWATI AMIR HUSIN ◽  
DWINITA W UTAMI ◽  
DAMAYANTI BUCHORI
Keyword(s):  
Population Structure ◽  
Molecular Markers ◽  
Helicoverpa Armigera ◽  
Random Mating ◽  
Molecular Characteristic ◽  
Pcr Analysis ◽  
Mating Pattern ◽  
Rapd Pcr ◽  
Helicoverpa Armígera

The effectiveness of this parasitoid was influenced by its population structure in the field. However, because this parasitoid has a tiny size, it was difficult to know the population structure of this parasitoid. This problem can be overcome by using molecular characteristic i.e. molecular markers. Based on RAPD-PCR analysis from 4 selected primers on 19 DNA samples from 3 different locations it was fond, that Gunung Bunder II population was divided into sub-population and so did Cugenang population, which is indicated by their small Fst and Nm index. The Fst and Nm index for Gunung Bunder II population was 0,39 and 0,77, while 0,51 and 0,47 for Cugenang population. If we calculated the Fst and Nm for all samples together, we found that this parasitoid has a random mating pattern, which is also shown by the dendrogram. The dendrogram indicate that each sub- opulation from one location was not grouped in one cluster but distributed in every cluster.

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