scholarly journals Identification of genetic differences between two species of morphologically identical Metapenaeus Genus from different environmental locations (Khaur Abdullah & Shatt Al- Arab) Southern of Iraq using (RFLP & RAPD) PCR molecular markers

2020 ◽  
Vol 5 (1) ◽  
pp. 015-021
Author(s):  
Alzuhairi Rabeeha
Keyword(s):  
Molecular Markers ◽  
Genetic Differences ◽  
Rapd Pcr ◽  
Rflp Rapd

Variation in Salmonella Enteritidis RAPD-PCR Patterns May Not Be Due to Genetic Differences

2011 ◽  
Vol 6 (4) ◽  
pp. e48-e49
Author(s):  
Demetrius L Mathis ◽  
Roy D Berghaus ◽  
Margie D Lee ◽  
John J Maurer
Keyword(s):  
Salmonella Enteritidis ◽  
Genetic Differences ◽  
Rapd Pcr

Variation in Salmonella Enteritidis RAPD-PCR Patterns May Not Be Due to Genetic Differences

2011 ◽  
Vol 55 (4) ◽  
pp. 620-625 ◽  
Author(s):  
Demetrius L. Mathis ◽  
Roy D. Berghaus ◽  
Margie D. Lee ◽  
John J. Maurer
Keyword(s):  
Salmonella Enteritidis ◽  
Genetic Differences ◽  
Rapd Pcr

scholarly journals Molecular Markers and Germplasm Enhancement: A Case Study in Tomato

HortScience ◽  
1996 ◽  
Vol 31 (4) ◽  
pp. 693a-693
Author(s):  
V. Meglic ◽  
R.T. Chetelat
Keyword(s):  
Molecular Markers ◽  
Species Group ◽  
Wild Relatives ◽  
Linkage Maps ◽  
Germplasm Enhancement ◽  
Solanum Lycopersicoides ◽  
Genetic Stocks ◽  
Rflp Rapd ◽  
Selection Of

The C.M. Rick Tomato Genetic Resources Center (TGRC) is a genebank of wild relatives, monogenic mutants, and miscellaneous genetic stocks of tomato. The wild species group includes representatives of all nine Lycopersicon spp., as well as four related Solanum species. One of the roles of the TGRC has been to foster the use of the widest available gene pool for tomato researchers. The wild nightshade Solanum lycopersicoides possesses a number of potentially useful traits, but has been untapped by breeders because of sterility and incompatibility barriers. We are using molecular markers to identify alien chromosomal segments introgressed from S. lycopersicoides into tomato. This project involves development of RFLP, RAPD, and isozyme marker linkage maps and their use in selection of homozygous segmental substitutions in backcross inbred progenies. In this fashion, a large proportion of the S. lycopersicoides genome has been integrated into the cultivated tomato. This study has also provided information on the nature of sterility and novel variation in hybrid derivatives.

scholarly journals PCR-based molecular markers for identification of taxa from the Calypogeia fissa complex (Jungermanniopsida, Calypogeiaceae)

2012 ◽  
Vol 28 (1) ◽  
pp. 9-18 ◽  
Author(s):  
Katarzyna Buczkowska ◽  
Patrycja Gonera ◽  
Bartosz Hornik
Keyword(s):  
Molecular Markers ◽  
Dna Sequences ◽  
Scar Marker ◽  
Issr Markers ◽  
Genetic Differences ◽  
Scar Markers ◽  
Sequence Characterized Amplified Region ◽  
Pcr Product ◽  
Isozyme Loci ◽  
Diagnostic Traits

Abstract Within Calypogeia fissa, two subspecies connected with geographic distribution are formally recognized: C. fissa subsp.fissa in Europe and C. fissa subsp.neogea in North America. Isoenzyme studies have shown that the European subspecies is genetically differentiated and composed of three genetically distinct groups PS, PB and G. The PS group has the most distinctive morphological features, but no morphological diagnostic traits have been found for groups PB and G. The sequence characterized amplified region (SCAR) markers developed on the basis of ISSR markers, applied in the study, allowed the delimitation of all groups distinguished in Europe within the C. fissa complex (PS, PB and G). The markers also revealed genetic differences between the European and American subspecies. Five primer pairs (Cal01, Cal03-Cal06) of the six pairs studied are useful as the diagnostic tool for the identification of particular groups from the C.fissa complex. The examined SCAR markers showed that the PS group of C.fissa subsp.fissa was the most distinct; it differed from both groups PB and G as well as from C.fissa subsp.neogea. All plants determined on the basis of diagnostic isozyme loci as the PS group amplified a longer product (380 bp) of the Cal04 primer pair than the rest of studied groups and yielded no amplification products in Cal03, Cal05 and Cal06 primers. The primer pair Cal03 distinguished the plants of the PB group from the remaining groups, since only the PB group generated a PCR product of about 290 bp. The genetic differences between all four studied groups of the C.fissa complex were supported by DNA sequences of the SCAR marker Cal04.

GENETIC TYPING OF LEUCONOSTOC MESENTEROIDES USING PCR

2020 ◽  
pp. 40-49
Author(s):  
A. Biruk ◽  
Y. Tarashkevich ◽  
N. Furik
Keyword(s):  
Phylogenetic Analysis ◽  
Genetic Heterogeneity ◽  
Leuconostoc Mesenteroides ◽  
Genetic Differences ◽  
Bootstrap Support ◽  
Genetic Typing ◽  
Rapd Pcr ◽  
High Level ◽  
Combined Methods

We studied the possibility of using RAPD-PCR with primers: ERIC1R-1, ERIC2-1, BOXA1R, BOXA2R and Rep-PCR with primers P15, P16, XD8, XD9, RAPD-mes, (GTG)5 to identify genetic heterogeneity of 9 strains and 8 isolates of Leuconostoc mesenteroides. Three clusters of cultures with a high level of bootstrap support were identified as a result of phylogenetic analysis obtained when typing Leuconostoc. The obtained results indicate the possibility of revealing genetic differences in the profile of the generated amplicons among Leuconostoc mesenteroides strains using the combined methods of Rep-PCR and RAPD-PCR.

scholarly journals Genetic Diversity Assessment of Luffa aegyptiaca Landraces Endemic in Egypt Based on Some Molecular Markers

2016 ◽  
Vol 26 (2) ◽  
pp. 209-217
Author(s):  
Mohammad Abdel Sttar Al Tahlawy ◽  
Mahmoud Abdel Aziz Ibrahim ◽  
Mohamed Ahmed Matter ◽  
Mervat El Sayed Mohamed ◽  
Mahmoud Mohamed Sakr
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Total Proteins ◽  
Pcr Marker ◽  
Polymorphic Loci ◽  
Average Percentage ◽  
Rapd Pcr ◽  
Diversity Assessment ◽  
Sds Page ◽  
Luffa Aegyptiaca

Luffa aegyptiaca is a popular climbing herb endemic in Egypt. We studied the genetic diversity among ten Luffa landraces (Cairo, Beni Suef, Menoufiya, Damietta, Banha, Aswan, Kafr el?Sheikh, Bir el?Abd, MarsaMatruh and Asyut) collected from different districts in Egypt. The results obtained from DNA fingerprinting revealed that there were polymorphic loci with average percentage of 44.6 among collected landraces whereas polymorphic loci obtained from SDS?PAGE were 23%. Discrimination between landraces was more efficient by using RAPD?PCR marker than total proteins SDS?PAGE which showed a limited level of intraspecific diversity.Plant Tissue Cult. & Biotech. 26(2): 209-217, 2016 (December)

Identification of molecular markers in soybean comparing RFLP, RAPD and AFLP DNA mapping techniques

1996 ◽  
Vol 14 (2) ◽  
pp. 156-169 ◽  
Author(s):  
Jhy-Jhu Lin ◽  
Jonathan Kuo ◽  
Jin Ma ◽  
James A. Saunders ◽  
Hunter S. Beard ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Dna Mapping ◽  
Mapping Techniques ◽  
Rflp Rapd

scholarly journals Decoding Evolution of Native Fishes in Garhwal Himalaya using Molecular Markers and DNA Barcoding

2020 ◽  
Vol 8 (10S2) ◽  
pp. 9-16
Keyword(s):  
Molecular Markers ◽  
Molecular Marker ◽  
Dna Barcoding ◽  
Animal Species ◽  
New Technologies ◽  
Fisheries Science ◽  
Long Time ◽  
Native Fishes ◽  
Rflp Rapd ◽  
Modern Era

As we are moving forward into the modern era of science, several new technologies have revolutionized various branches of science. Techniques of biodiversity conservation, fish biology etc. has also adapted to modern techniques. For a long time, most of the researches in taxonomy, including fisheries science were based on morphology and traditional methods. After the decade of 90’s, slowly severalmolecular markers like RFLP, RAPD, SNP’s etc. made inroad into taxonomy and fisheries. Molecular markers have several applications in the field of livestock improvement and understanding population dynamics to name a few. Since the 2004, a specific molecular marker, generally known as DNA Barcoding for species identification, came up. This molecular marker is a part of mitochondrial genome that encodes for Cytochrome C Oxidase Unit I (also called as COX or COI). It is advantageous because it has been tested across several animal species and it can differentiate species very well. This marker has also been used as a forensic tool to identify the species. In the current paper, we have used this molecular marker to decode evolution of native fishes of Garhwal Himalayan region. Over 350 barcodes were developed and these barcodes were used to for phylogenetic analysis.

scholarly journals Pengelompokan dan Struktur Populasi Parasitoid Telur Trichogrammatoidea armigera pada Telur Helicoverpa armigera pada Jagung Berdasarkan Karakter Molekuler

2015 ◽  
Vol 7 (1) ◽  
pp. 54
Author(s):  
BAHAGIAWATI AMIR HUSIN ◽  
DWINITA W UTAMI ◽  
DAMAYANTI BUCHORI
Keyword(s):  
Population Structure ◽  
Molecular Markers ◽  
Helicoverpa Armigera ◽  
Random Mating ◽  
Molecular Characteristic ◽  
Pcr Analysis ◽  
Mating Pattern ◽  
Rapd Pcr ◽  
Helicoverpa Armígera

The effectiveness of this parasitoid was influenced by its population structure in the field. However, because this parasitoid has a tiny size, it was difficult to know the population structure of this parasitoid. This problem can be overcome by using molecular characteristic i.e. molecular markers. Based on RAPD-PCR analysis from 4 selected primers on 19 DNA samples from 3 different locations it was fond, that Gunung Bunder II population was divided into sub-population and so did Cugenang population, which is indicated by their small Fst and Nm index. The Fst and Nm index for Gunung Bunder II population was 0,39 and 0,77, while 0,51 and 0,47 for Cugenang population. If we calculated the Fst and Nm for all samples together, we found that this parasitoid has a random mating pattern, which is also shown by the dendrogram. The dendrogram indicate that each sub- opulation from one location was not grouped in one cluster but distributed in every cluster.

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