scholarly journals Antidiarrheal activity of methanolic extract of the root bark of Cordia africana

2016 ◽  
Vol Volume 8 ◽  
pp. 53-59 ◽  
Author(s):  
Assefa Belay Asrie ◽  
Mohammedbrhan Abdelwuhab ◽  
Zewdneh Shewamene ◽  
Desalegn Asmelashe Gelayee ◽  
Getnet Mequanint Adinew ◽  
...  
Keyword(s):  
Methanolic Extract ◽  
Root Bark ◽  
Antidiarrheal Activity ◽  
Cordia Africana

scholarly journals Evaluation of Antidiarrheal Activity of 80% Methanolic Extract of the Leaves of Cordia africana (Lamiaceae) in Mice

2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
Yared Andargie Ferede ◽  
Woretaw Sisay Zewdu ◽  
Mulugeta Molla Zeleke ◽  
Muluken Adela Alemu
Keyword(s):  
Castor Oil ◽  
Diarrheal Disease ◽  
Methanolic Extract ◽  
Gastrointestinal Transit ◽  
Screening Tests ◽  
Phytochemical Screening ◽  
Acute Toxicity Study ◽  
Single Oral Administration ◽  
Antidiarrheal Activity ◽  
Cordia Africana

Background. Diarrheal disease is a major cause of morbidity and mortality throughout the world, particularly in developing countries. Currently available drugs are linked with adverse effects, contraindications, and risk of resistance. Traditionally, the leaf concoction of Cordia africana is claimed to be used for diarrhea. However, the safety and efficacy of the leaf extract have not been scientifically approved yet. Therefore, the study was conducted to validate its antidiarrheal activity and safety profile in mice. Method. The hydromethanolic extract was obtained by the cold maceration technique in 80% methanol. Phytochemical screening tests were done for secondary metabolites by using standard tests. The antidiarrheal activity of the test extract at the doses of 100, 200, and 400 mg/kg was evaluated by using castor oil-induced diarrheal, gastrointestinal transit, and enteropooling models in mice. Result. In an acute toxicity study, there were no visible signs of toxicity and mortality following a single oral administration of 2000 mg/kg. Phytochemical screening tests revealed the presence of alkaloids, saponins, flavonoids, terpenoids, phenols, and tannins. The hydromethanolic extract significantly prolonged the onset of diarrhea and reduced the weight of wet and total feces at 100 ( P < 0.01 ), 200 ( P < 0.001 ), and 400 mg/kg ( P < 0.001 ) in the castor oil-induced diarrheal model. However, in the gastrointestinal transit model, a significant ( P < 0.001 ) reduction in the charcoal meal travel was observed in the middle (200 mg/kg) and higher (400 mg/kg) test doses. Similarly, the extract produced a significant ( P < 0.001 ) reduction in the weight and volume of intestinal contents at the aforementioned doses. Conclusion. The study demonstrated that the test extract showed promising antidiarrheal activity. Hence, this study supports its antidiarrheal use in Ethiopian folklore medicine.

Antidiarrheal activity of Bridelia ferruginea bark methanolic extract involves modulation ATPases in mice and inhibition of muscarinic acetylcholine receptor (M3) and prostaglandin E2 receptor 3 (EP3) in silico

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Blessing Olugbamila Omolaso ◽  
Julius Kolawole Adesanwo ◽  
Ahmed Adebayo Ishola ◽  
Adeoti Gbemisola Adegoke ◽  
Francis O. Akingbule ◽  
...  
Keyword(s):  
Molecular Docking ◽  
Prostaglandin E2 ◽  
Acetylcholine Receptor ◽  
Methanolic Extract ◽  
Muscarinic Acetylcholine Receptor ◽  
Gastrointestinal Transit ◽  
Muscarinic Acetylcholine ◽  
Bridelia Ferruginea ◽  
Antidiarrheal Activity ◽  
Prostaglandin E2 Receptor

Abstract Objectives Diarrhea, an abnormal state in which the individual has about three or more daily bowel movements, is now considered one of the most challenging global public health problems. Using plant products, such as Bridelia ferruginea is an alternative treatment option. The objective of this study was to investigate the antidiarrheal activity of B. ferruginea bark methanolic extract (BfME) and the mechanisms involved. Methods BfME antidiarrheal activity was evaluated in mice model of castor oil-induced diarrhea and enteropooling. To evaluate motility, gastrointestinal transit time was carried out using phenol red meal, while intestinal activities of selected ATPases were also evaluated. Furthermore, the active components in BfME were detected by GC-MS analysis, while molecular docking of the most abundant compounds with muscarinic acetylcholine receptor (M3) and prostaglandin E2 receptor 3 (EP3) were conducted. Results BfME at 400 and 800 mg/kg showed antidiarrheal activity by delaying onset of diarrhea, reduced gastrointestinal transit and increased intestinal activities of Na+ K+-ATPase, Ca2+ Mg2+-ATPase and Mg2+-ATPase. Molecular docking revealed that γ-sitosterol, α-amyrin, and stigmasterol have outstanding binding affinity for M3 and EP3. Conclusions In view of these results, the observed antidiarrheal activity possibly occurs via the activation of ATPases activities and inhibition of M3 and EP3.

Isolation of Stigmasterol and β-Sitosterol from Methanolic Extract of Root Bark of Calotropis gigantea (Linn)

2007 ◽  
Vol 10 (22) ◽  
pp. 4174-4176 ◽  
Author(s):  
M. Rowshanul ◽  
Farjana Nikkon . ◽  
Matiar Rahman . ◽  
M. Ekramul Haque . ◽  
M. Rezaul Karim .
Keyword(s):  
Methanolic Extract ◽  
Root Bark ◽  
Calotropis Gigantea

scholarly journals The Triterpene Constituents of the Root Bark of a Hybrid between Morus alba L. and M. rotundiloba Koidz. and its Antityrosinase Activities

2007 ◽  
Vol 2 (4) ◽  
pp. 1934578X0700200 ◽  
Author(s):  
Nisakarn Pianwijanpong ◽  
Narongchai Pongpan ◽  
Leena Suntornsuk ◽  
Omboon Luanratana
Keyword(s):  
Betulinic Acid ◽  
Methanolic Extract ◽  
Limit Of Detection ◽  
Linear Regression Analysis ◽  
Morus Alba ◽  
Bark Extract ◽  
Root Bark ◽  
Limit Of Quantification ◽  
Linear Coefficient ◽  
Spectroscopic Analyses

Mulberry plants have various traditional uses in Thailand, China, Korea and Vietnam. Recently, the root bark of Morus alba L. is used as whitening ingredient in various skin preparations. This paper describes the isolation of two triterpenes from the root bark of a 5 years old hybrid between M. alba L. and M. rotundiloba Koidz., a mulberry sericulture NM60 which is widely grown in Thailand for tea and silk industries. Pentacyclic triterpenes, α-amyrin acetate and betulinic acid, were isolated and identified, their structures were elucidated on the basis of spectroscopic analyses. The antityrosinase activities were tested having the IC50 values of 1.57 mM and 0.50 mM respectively. A validated HPLC technique for betulinic acid was also reported. Linear regression analysis of betulinic acid fell within the range of 61.25-409.15 μg/mL with a linear coefficient of 0.9992. The percentage of recovery is in accordance with that specified by the USP. The limit of detection and limit of quantification were 14.7 and 61.25 μg/mL, respectively. The analysis of the crude methanolic extract revealed 0.14% w/w betulinic acid in the root bark. These findings promote the use of the root bark extract of Thai mulberry hybrid in whitening cosmetics using betulinic acid as a marker and promote further study for the development of antimelanoma agent.

scholarly journals In vitro carminative and in vivo antidiarrheal activity of Citrus maxima

2021 ◽  
Vol 10 (5) ◽  
pp. 286-288
Author(s):  
Cheruku Gowtham Reddy ◽  
◽  
Yalamarthi Akshaya Shilpa ◽  
Anumula Pavani ◽  
Adabala Divya ◽  
...  
Keyword(s):  
Methanolic Extract ◽  
Significant Activity ◽  
Methanolic Extracts ◽  
Soxhlet Method ◽  
Citrus Maxima ◽  
Electrolyte Loss ◽  
Antidiarrheal Activity ◽  
Titration Technique

In this study, the methanolic extracts of leaves of Citrus maxima were evaluated for their carminative and antidiarrheal activities. By soxhlet method, the methanolic extract of leaves of Citrus maxima was prepared. Carminative activity of methanolic extract was studied in vitro by acid-base titration technique and antidiarrheal activity was studied in rats by castor oil induced diarrhoea method. The phytochemical screening was done using various chemical tests. Alkaloids, tannins, carbohydrates, flavonoids, terpenoids, amino acids, anthraquinones, glycosides are confirmed to be present in the leaf. Carminative activity of extract was found significantly less with 5mL, when compared with 10mL extract. The antidiarrheal activity observed in the animals treated with 200mg/kg showed significant activity compared with the animals treated with 100mg/kg. The study revealed that carminative activity and antidiarrheal activity of methanolic extract of C. maxima. May be the extract acts on circular and longitudinal intestinal muscle by inhibiting acetylcholine and prostaglandins, which reduces peristaltic movements and prolongs transmit time, reduces fecal volume, increases viscosity and viscous fluid and electrolyte loss. The compound in the extract causes mild irritation resulting in vasodilation of gastrointestinal muscles, thereby relieving cramping and expels gas

Isoflavonoids from the Root Bark of Piscidia erythrina and a Note on the Structure of Piscidone

1989 ◽  
Vol 44 (11-12) ◽  
pp. 905-913 ◽  
Author(s):  
John L. Ingham ◽  
Satoshi Tahara ◽  
Seiji Shibaki ◽  
Junya Mizutani
Keyword(s):  
Methanolic Extract ◽  
Partial Characterization ◽  
Bark Extract ◽  
Root Bark ◽  
Chemical Methods ◽  
H Nmr

Abstract A methanolic extract of Piscidia erythrina root bark has been found to contain various isofla-vonoids including rotenone (rotenoid), lisetin (coum aronochrom one) and six known isoflavones (ichthynone, piscidone, piscerythrone, 2′-deoxypiscerythrone, 6′-prenylpiscerythrone and 3′,5′-diprenylgenistein). The extract additionally yielded three new 5-hydroxyisoflavones (piscery-thrinetin, 2′-hydroxypiscerythrinetin and isow ighteone) and a previously unreported coum arono­ chrom one (8-prenyl-lisetin). All four com pounds were identified using a com bination of spectro­ scopic (UV , MS, 1H NMR) and chemical methods. Although several other 5-hydroxyisoflavones were also isolated from the root bark extract, the quantities of each were sufficient only to permit their partial characterization. Structure 2 for piscidone has been confirmed by 1H NMR spectros­ copy.

Oral treatment with methanolic extract of the root bark of Condalia buxifolia Reissek alleviates acute pain and inflammation in mice: Potential interactions with PGE2, TRPV1/ASIC and PKA signaling pathways

2016 ◽  
Vol 185 ◽  
pp. 319-326 ◽  
Author(s):  
Róli Rodrigues Simões ◽  
Igor dos Santos Coelho ◽  
Caroline Cunha do Espírito Santo ◽  
Ademir Farias Morel ◽  
Eliane Maria Zanchet ◽  
...  
Keyword(s):  
Signaling Pathways ◽  
Acute Pain ◽  
Methanolic Extract ◽  
Oral Treatment ◽  
Root Bark ◽  
Potential Interactions
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