scholarly journals Coupling of a bifunctional peptide R13 to OTMCS-PEI copolymer as a gene vector increases transfection efficiency and tumor targeting

2014 ◽  
pp. 1311 ◽  
Author(s):  
Kehai Liu ◽  
Lv Hui ◽  
Zhu Qing ◽  
Liu Kewu ◽  
Zhu Manman ◽  
...  
Keyword(s):  
Tumor Targeting ◽  
Transfection Efficiency ◽  
Gene Vector

Biodegradable nano-organosilica gene carrier for high-efficiency gene transfection

2020 ◽  
Vol 8 (12) ◽  
pp. 2483-2494
Author(s):  
Kun Zeng ◽  
Li Ma ◽  
Wenxiu Yang ◽  
Shan Lei ◽  
Mozhen Wang ◽  
...  
Keyword(s):  
High Efficiency ◽  
Transfection Efficiency ◽  
Gene Transfection ◽  
Gene Carrier ◽  
Gene Vector ◽  
High Transfection Efficiency ◽  
In Cells

Guanidinated-fluorinated α-polylysine-modified organosilica nanoparticles can form a novel raisin-bread-like gene vector, which is disintegrated in cells by GSH to show high transfection efficiency.

scholarly journals Binding Affinity, Cellular Uptake, and Subsequent Intracellular Trafficking of the Nano-Gene Vector P123-PEI-R13

2016 ◽  
Vol 2016 ◽  
pp. 1-8
Author(s):  
Yaguang Zhang ◽  
Hongmei Shu ◽  
Jing Hu ◽  
Min Zhang ◽  
Junweng Wu ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Cellular Uptake ◽  
Intracellular Trafficking ◽  
Transfection Efficiency ◽  
Cross Linking ◽  
Low Molecular Weight ◽  
Gene Vector ◽  
Dna Complexes ◽  
Dynamic Source

A nano-gene vector PEI-P123-R13 was synthesized by cross-linking low molecular weight PEI with P123 and further coupling bifunctional peptide R13 to the polymer for targeting tumor and increasing cellular uptake. The binding assessment of R13 toαvβ3 positive cells was performed by HRP labeling. The internalization pathways of P123-PEI-R13/DNA complexes were investigated based on the effect of specific endocytic inhibitors on transfection efficiency. The mechanism of intracellular trafficking was investigated based on the effect of endosome-lysosome acidification inhibitors, cytoskeleton, and dynein inhibitors on transfection efficiency. The results indicated that the bifunctional peptide R13 had the ability of binding toαvβ3 positive cellsin vitro. The modification of P123-PEI-R13 with R13 made it display new property of internalization. P123-PEI-R13/DNA complexes were conducted simultaneously via clathrin-mediated endocytosis, caveolin-mediated endocytosis, macropinocytosis, and possible energy-independent route. After internalization, P123-PEI-R13/DNA complexes could escape from the endosome-lysosome system because of its acidification and further took microtubule as the track and dynein as the dynamic source to be transported toward the microtubule (+) end, to wit nucleus, under the action of microfilament, and with the aid of intermediate filament.

scholarly journals Endosomal explosion induced by hypertonicity enhances chitosan transfection

2019 ◽  
Author(s):  
Shupeng Wang ◽  
Shaohua Jin ◽  
Guangzhi Li ◽  
Rui Sun ◽  
Qinghai Shu ◽  
...  
Keyword(s):  
Cell Lines ◽  
Cell Function ◽  
Transfection Efficiency ◽  
Low Cost ◽  
Research Field ◽  
Gene Vector ◽  
Practical Strategy ◽  
Efficient Delivery ◽  
High Efficient ◽  
Short Time

AbstractTransferring DNA into cells to regulate cell function is a novel research field in recent decades. Chitosan is a gene vector with the properties of low-cost and safe, but high efficient delivery has remained challenging. We developed a strategy termed EEIH, for endosomal explosion induced by hypertonicity, in which short-time exposure to hypertonic solutions triggers endosomes destabilization like explosions. EEIH can force chitosan/DNA polyplexes to break through the endosomal barriers to approach the nucleus, which results in boosting the transfection efficiency of chitosan in several cell lines. We demonstrate that EEIH is a significant and practical strategy in chitosan transfection system without sophisticated modification of chitosan.

Acid-sensitive poly(β-cyclodextrin)-based multifunctional supramolecular gene vector

2018 ◽  
Vol 9 (4) ◽  
pp. 450-462 ◽  
Author(s):  
Yunti Zhang ◽  
Qimin Jiang ◽  
Marcin Wojnilowicz ◽  
Shuaijun Pan ◽  
Yi Ju ◽  
...  
Keyword(s):  
Transfection Efficiency ◽  
Gene Vector ◽  
Low Cytotoxicity

Multifunctional host–guest supramolecular PCD-acetal-PGEA/Ad-PEG-FA polyplexes showing FA-targeting and acid-triggered intracellular gene release resulted in good transfection efficiency and low cytotoxicity.

Novel Thermoresponsive Nonviral Gene Vector: P(NIPAAm-co-NDAPM)-b-PEI with Adjustable Gene Transfection Efficiency

2008 ◽  
Vol 19 (7) ◽  
pp. 1368-1374 ◽  
Author(s):  
Han Cheng ◽  
Jing-Ling Zhu ◽  
Yun-Xia Sun ◽  
Si-Xue Cheng ◽  
Xian-Zheng Zhang ◽  
...  
Keyword(s):  
Transfection Efficiency ◽  
Gene Transfection ◽  
Gene Vector

scholarly journals Protein Liposomes-Mediated Targeted Acetylcholinesterase Gene Delivery for Effective Liver Cancer Therapy

2021 ◽  
Author(s):  
Kai Wang ◽  
Fusheng Shang ◽  
Dagui Chen ◽  
Tieliu Cao ◽  
Xiaowei Wang ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Cancer Therapy ◽  
Gene Delivery ◽  
Liver Cancer ◽  
Targeted Delivery ◽  
Tumor Targeting ◽  
Transfection Efficiency ◽  
Subcutaneous Administration ◽  
Viral Gene ◽  
Functional Protein

Abstract Effective methods to deliver therapeutic genes to solid tumors and improve their bioavailability are the main challenges of current medical research on gene therapy. The development of efficient non-viral gene vector with tumor-targeting has very important application value in the field of cancer therapy. Proteolipid integrated with tumor-targeting potential of functional protein and excellent gene delivery performance has shown potential for targeted gene therapy.Results: Herein, we prepared transferrin-modified liposomes (Tf-PL) for the targeted delivery of acetylcholinesterase (AChE) therapeutic gene to liver cancer. We found that the derived Tf-PL/AChE liposomes exhibited much higher transfection efficiency than the commercial product Lipo 2000 and shown premium targeting efficacy to liver cancer SMMC-7721 cells in vitro. In vivo, the Tf-PL/AChE could effectively target liver cancer, and significantly inhibit the growth of liver cancer xenografts grafted in nude mice by subcutaneous administration. Conclusion: This study proposed a transferrin-modified proteolipid-mediated gene delivery strategy for targeted liver cancer treatment, which has a promising potential for precise personalized cancer therapy.

Effect of molecular weight on the transfection efficiency of novel polyurethane as a biodegradable gene vector

2006 ◽  
Vol 77A (4) ◽  
pp. 736-746 ◽  
Author(s):  
Min-Da Shau ◽  
S-Ja Tseng ◽  
Tsung-Fu Yang ◽  
Jong-Yuh Cherng ◽  
Wei-Kuo Chin
Keyword(s):  
Molecular Weight ◽  
Transfection Efficiency ◽  
Gene Vector

Tumor Targeting Gene Vector for Visual Tracking of Bcl-2 siRNA Transfection and Anti-Tumor Therapy

2020 ◽  
Vol 12 (9) ◽  
pp. 10193-10201 ◽  
Author(s):  
Wan Sun ◽  
Xu-Ying Liu ◽  
Le-Le Ma ◽  
Zhong-Lin Lu
Keyword(s):  
Visual Tracking ◽  
Tumor Targeting ◽  
Tumor Therapy ◽  
Gene Vector ◽  
Sirna Transfection
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