Novel Thermoresponsive Nonviral Gene Vector: P(NIPAAm-co-NDAPM)-b-PEI with Adjustable Gene Transfection Efficiency

2008 ◽  
Vol 19 (7) ◽  
pp. 1368-1374 ◽  
Author(s):  
Han Cheng ◽  
Jing-Ling Zhu ◽  
Yun-Xia Sun ◽  
Si-Xue Cheng ◽  
Xian-Zheng Zhang ◽  
...  
Keyword(s):  
Transfection Efficiency ◽  
Gene Transfection ◽  
Gene Vector

Biodegradable nano-organosilica gene carrier for high-efficiency gene transfection

2020 ◽  
Vol 8 (12) ◽  
pp. 2483-2494
Author(s):  
Kun Zeng ◽  
Li Ma ◽  
Wenxiu Yang ◽  
Shan Lei ◽  
Mozhen Wang ◽  
...  
Keyword(s):  
High Efficiency ◽  
Transfection Efficiency ◽  
Gene Transfection ◽  
Gene Carrier ◽  
Gene Vector ◽  
High Transfection Efficiency ◽  
In Cells

Guanidinated-fluorinated α-polylysine-modified organosilica nanoparticles can form a novel raisin-bread-like gene vector, which is disintegrated in cells by GSH to show high transfection efficiency.

scholarly journals One-Step Formation of Chondrocytes through Direct Reprogramming via Polysaccharide-Based Gene Delivery

2019 ◽  
Vol 2019 ◽  
pp. 1-12 ◽  
Author(s):  
Qingtong Yu ◽  
Yan Wang ◽  
Xia Cao ◽  
Wenwen Deng ◽  
Michael Adu Frimpong ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Western Blot ◽  
Toluidine Blue ◽  
Transfection Efficiency ◽  
Gene Transfection ◽  
Single Gene ◽  
Porphyra Yezoensis ◽  
Gene Vector ◽  
Collagen Ii ◽  
Safranin O

An innovative strategy for the generation of chondrocytes was thoroughly studied in this paper. Polyetherimide-modified polysaccharides of Porphyra yezoensis (pmPPY) served as a nonviral gene vector and delivered Sox9 plasmid to directly reprogram mouse embryonic fibroblasts into chondrocytes. The gene transfer efficiency was evaluated through ELISA, RT-PCR, and Western blot. The induced chondrocytes were identified through toluidine blue, Safranin O, and the immunostaining. The expression level of collagen II was finally evaluated through western blot. The pSox9/pmPPY nanoparticles (1:50) showed lower cytotoxicity as well as greater gene transfection efficiency than Lipofectamine 2000 and polyetherimide (PEI) (p<0.05). The results of toluidine blue, Safranin O, and the immunostaining of collagen II further showed that the normal MEFs were successfully reprogrammed into chondrocytes. These findings indicate that pmPPY could be a promising gene vector for the generation of chondrocytes via single-gene delivery strategy, which might provide abundant chondrocytes for cartilage repair.

Non-Viral Vectors for Gene Delivery

2018 ◽  
Vol 9 (1) ◽  
pp. 4-11 ◽  
Author(s):  
Aparna Bansal ◽  
Himanshu
Keyword(s):  
Gene Therapy ◽  
Viral Vectors ◽  
Transfection Efficiency ◽  
Gene Transfection ◽  
Expression System ◽  
Target Cells ◽  
Specific Expression ◽  
Naked Dna

Introduction: Gene therapy has emerged out as a promising therapeutic pave for the treatment of genetic and acquired diseases. Gene transfection into target cells using naked DNA is a simple and safe approach which has been further improved by combining vectors or gene carriers. Both viral and non-viral approaches have achieved a milestone to establish this technique, but non-viral approaches have attained a significant attention because of their favourable properties like less immunotoxicity and biosafety, easy to produce with versatile surface modifications, etc. Literature is rich in evidences which revealed that undoubtedly, non–viral vectors have acquired a unique place in gene therapy but still there are number of challenges which are to be overcome to increase their effectiveness and prove them ideal gene vectors. Conclusion: To date, tissue specific expression, long lasting gene expression system, enhanced gene transfection efficiency has been achieved with improvement in delivery methods using non-viral vectors. This review mainly summarizes the various physical and chemical methods for gene transfer in vitro and in vivo.

Ultrasound molecular imaging-guided tumor gene therapy through dual-targeted cationic microbubbles

2021 ◽  
Vol 9 (7) ◽  
pp. 2454-2466
Author(s):  
Yingying Liu ◽  
Yuli Zhou ◽  
Jinfeng Xu ◽  
Hui Luo ◽  
Yao Zhu ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Molecular Imaging ◽  
Transfection Efficiency ◽  
Gene Transfection ◽  
Tumor Gene Therapy ◽  
Ultrasound Molecular Imaging ◽  
Tumor Gene

A novel dual-targeted cationic microbubbles help to improve gene transfection efficiency.

Retracted Article: A multifunctional self-dissociative polyethyleneimine derivative coating polymer for enhancing the gene transfection efficiency of DNA/polyethyleneimine polyplexes in vitro and in vivo

2015 ◽  
Vol 6 (5) ◽  
pp. 780-796 ◽  
Author(s):  
Cheng Wang ◽  
Xiuli Bao ◽  
Xuefang Ding ◽  
Yang Ding ◽  
Sarra Abbad ◽  
...  
Keyword(s):  
Transfection Efficiency ◽  
Gene Transfection ◽  
Retracted Article ◽  
First Time

A novel coating polymer LPHF is developed for the first time to elevate the transfection efficiency of DP binary polyplexes in vitro and in vivo.

scholarly journals Enhanced gene transfection efficiency by low-dose 25 kDa polyethylenimine by the assistance of 1.8 kDa polyethylenimine

Drug Delivery ◽  
2018 ◽  
Vol 25 (1) ◽  
pp. 1740-1745 ◽  
Author(s):  
Hui Zhang ◽  
Zhiyi Chen ◽  
Meng Du ◽  
Yue Li ◽  
Yuhao Chen
Keyword(s):  
Low Dose ◽  
Transfection Efficiency ◽  
Gene Transfection

A PEG-b-poly(disulfide-l-lysine) based redox-responsive cationic polymer for efficient gene transfection

2019 ◽  
Vol 7 (11) ◽  
pp. 1893-1905 ◽  
Author(s):  
Ihsan Ullah ◽  
Jing Zhao ◽  
Shah Rukh ◽  
Khan Muhammad ◽  
Jintang Guo ◽  
...  
Keyword(s):  
Gene Transfection ◽  
Cationic Polymer ◽  
Endosomal Escape ◽  
Gene Vector ◽  
Efficient Gene ◽  
Redox Responsive ◽  
Responsive Gene

A redox responsive gene vector efficiently delivered pDNA with a good internalization tendency, efficient endosomal escape and high nucleus internalization.

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