scholarly journals Using the air pouch model for assessing in vivo inflammatory activity of nanoparticles

2014 ◽  
pp. 1105 ◽  
Author(s):  
Denis Girard
Keyword(s):  
Inflammatory Activity ◽  
Air Pouch ◽  
Air Pouch Model

Insulin Derived Fibrils Induce Cytotoxicity in vitro and Trigger Inflammation in Murine Models

2021 ◽  
pp. 193229682110338
Author(s):  
Brianne E. Lewis ◽  
Adam Mulka ◽  
Li Mao ◽  
Roshanak Sharafieh ◽  
Yi Qiao ◽  
...  
Keyword(s):  
Tissue Reaction ◽  
Inflammatory Activity ◽  
Dependent Diabetes Mellitus ◽  
Air Pouch ◽  
Tissue Reactions ◽  
And Function ◽  
Air Pouch Model ◽  
Insulin Fibrils

Background: Effective exogenous insulin delivery is the cornerstone of insulin dependent diabetes mellitus management. Recent literature indicates that commercial insulin-induced tissue reaction and cellular cytotoxicity may contribute to variability in blood glucose as well as permanent loss of injection or infusion site architecture and function. It is well accepted that insulin formulations are susceptible to mechanical and chemical stresses that lead to insulin fibril formation. This study aims to characterize in vitro and in vivo toxicity, as well as pro-inflammatory activity of insulin fibrils. Method: In vitro cell culture evaluated cytotoxicity and fibril uptake by macrophages and our modified murine air-pouch model quantified inflammatory activity. The latter employed FLOW cytometry and histopathology to characterize fibril-induced inflammation in vivo, which included fibril uptake by inflammatory phagocytes. Results: These studies demonstrated that insulin derived fibrils are cytotoxic to cells in vitro. Furthermore, inflammation is induced in the murine air-pouch model in vivo and in response, macrophages uptake fibrils both in vitro and in vivo. Conclusions: Administration of insulin fibrils can lead to cytotoxicity in macrophages. In vivo data demonstrate insulin fibrils to be pro-inflammatory which over time can lead to cumulative cell/tissue toxicity, inflammation, and destructive wound healing. Long term, these tissue reactions could contribute to loss of insulin injection site architecture and function.

scholarly journals Interleukin (IL)-4 Induces Leukocyte Infiltration In Vivo by an Indirect Mechanism

2009 ◽  
Vol 2009 ◽  
pp. 1-10 ◽  
Author(s):  
Claude Ratthé ◽  
Jamila Ennaciri ◽  
David M. Garcês Gonçalves ◽  
Sonia Chiasson ◽  
Denis Girard
Keyword(s):  
Mrna Expression ◽  
Cell Populations ◽  
Antibody Array ◽  
Leukocyte Infiltration ◽  
Air Pouch ◽  
Indirect Mechanism ◽  
Ccl2 Mrna ◽  
Raw264.7 Macrophage ◽  
Air Pouch Model

Interleukin (IL)-4 is a cytokine known mainly for its anti-inflammatory activity. Using the in vivo murine air pouch model, we found that IL-4 significantly increased the number of leukocytes after 9 hours of treatment, consisting mainly of neutrophil (60%) and monocytic (40%) cell populations. Using an antibody array, we found that the expression of several analytes (predominantly CCL2) was increased by IL-4 before the arrival of leukocytes. The IL-4-induced expression of CCL-2 was confirmed by ELISA. Air pouch resident lining cells were harvested and were found to express IL-4Rα. CCL2 mRNA expression was monitored in lining cells, cells isolated from the air pouch skin, in RAW264.7 macrophage and in epithelial Mode-K cells and its expression was increased in response to IL-4 in all conditions. We conclude that IL-4 can attract leukocytes in vivo by an indirect mechanism involving the production of several analytes by, at least, resident cells.

Investigations of Anti-Inflammatory Activity of a Peptide-Based Hydrogel Using Rat Air Pouch Model

2018 ◽  
Vol 11 (3) ◽  
pp. 2849-2859 ◽  
Author(s):  
Pramod K. Gavel ◽  
Hamendra S. Parmar ◽  
Versha Tripathi ◽  
Narendra Kumar ◽  
Ankan Biswas ◽  
...  
Keyword(s):  
Inflammatory Activity ◽  
Air Pouch ◽  
Anti Inflammatory ◽  
Air Pouch Model

scholarly journals Suppressions of Serotonin-Induced Increased Vascular Permeability and Leukocyte Infiltration byBixa orellanaLeaf Extract

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Yoke Keong Yong ◽  
NurShahira Sulaiman ◽  
Muhammad Nazrul Hakim ◽  
Gwendoline Ee Cheng Lian ◽  
Zainul Amirudin Zakaria ◽  
...  
Keyword(s):  
Vascular Permeability ◽  
Inflammatory Activity ◽  
Blue Dye ◽  
Leukocyte Infiltration ◽  
Permeability Model ◽  
Air Pouch ◽  
Rat Paw Edema ◽  
Anti Inflammatory ◽  
Pharmacological Screening ◽  
Air Pouch Model

The aim of the present study was to evaluate the anti-inflammatory activities of aqueous extract ofBixa orellana(AEBO) leaves and its possible mechanisms in animal models. The anti-inflammatory activity of the extract was evaluated using serotonin-induced rat paw edema, increased peritoneal vascular permeability, and leukocyte infiltrations in an air-pouch model. Nitric oxide (NO), indicated by the sum of nitrites and nitrates, and vascular growth endothelial growth factor (VEGF) were measured in paw tissues of rats to determine their involvement in the regulation of increased permeability. Pretreatments with AEBO (50 and 150 mg kg−1) prior to serotonin inductions resulted in maximum inhibitions of 56.2% of paw volume, 45.7% of Evans blue dye leakage in the peritoneal vascular permeability model, and 83.9% of leukocyte infiltration in the air-pouch model. 57.2% maximum inhibition of NO and 27% of VEGF formations in rats’ paws were observed with AEBO at the dose of 150 mg kg−1. Pharmacological screening of the extract showed significant (P<0.05) anti-inflammatory activity, indicated by the suppressions of increased vascular permeability and leukocyte infiltration. The inhibitions of these inflammatory events are probably mediated via inhibition of NO and VEGF formation and release.

scholarly journals Anti-Inflammatory Activity of the Essential Oil Citral in Experimental Infection withStaphylococcus aureusin a Model Air Pouch

2017 ◽  
Vol 2017 ◽  
pp. 1-10 ◽  
Author(s):  
Hellen Braga Martins ◽  
Nathan das Neves Selis ◽  
Clarissa Leal Silva e Souza ◽  
Flávia S. Nascimento ◽  
Suzi Pacheco de Carvalho ◽  
...  
Keyword(s):  
Essential Oil ◽  
Blood Cells ◽  
Mutual Influence ◽  
White Blood Cells ◽  
Local Treatment ◽  
Inflammatory Activity ◽  
Air Pouch ◽  
Qpcr Array ◽  
Anti Inflammatory ◽  
Air Pouch Model

This study proposes to implement an alternative and effective strategy for local treatment of disease provoked byS. aureus. For the analysis of possible anti-inflammatory activity of essential oil, after establishing an air pouch model, 48 male mice of Balb/c were treated, infected, and euthanized at 4 and 8 h. Thus, the total and differential white blood cells were counted in the animal’s blood, and cytokines IL-1β, IL-6, and TNF-αwere titrated using ELISA in the air pouch lavage. Moreover, TNF-α, IL-1β, and IL-6 gene expression was analyzed through an RT-qPCR array, andS. aureuswas quantified using qPCR. Our results,p<0.05, showed that EOC reduced the quantity of microorganisms. The group of mice treated with essential oil citral showed a significant decrease in TNF-αlevels in tests demonstrating anti-inflammatory activity. There is no data about the mutual influence of the air pouch model, essential oil citral, andS. aureus. Thus, considering the interaction of these variables and the anti-inflammatory activity of the essential oil citral, we demonstrated, by alternative local treatment, a new antimicrobial agent that is not an antibiotic.

Jobelyn® exhibited anti-inflammatory, antioxidant, and membrane-stabilizing activities in experimental models

2015 ◽  
Vol 26 (5) ◽  
Author(s):  
Solomon Umukoro ◽  
Oluwafemi Gabriel Oluwole ◽  
Anthony T. Eduviere ◽  
Omogbiya Itievere Adrian ◽  
Abayomi M. Ajayi
Keyword(s):  
Chronic Inflammation ◽  
Acute Inflammation ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Experimental Models ◽  
Hypotonic Medium ◽  
Air Pouch ◽  
Anti Inflammatory ◽  
Air Pouch Model

AbstractJobelynAcute inflammation was induced with intraplanter injection of carrageenan and increase in rat paw volume was measured using plethysmometer. The volume of fluid exudates, number of leukocytes, concentrations of malondialdehyde (MDA), and glutathione (GSH) in the fluid were measured on day 5 after induction of chronic inflammation with carrageenan in the granuloma air pouch model. RBC lysis induced by hypotonic medium as determined by release of hemoglobin was measured spectrophotometerically.JB (50–200 mg/kg) given orally produced a significant inhibition of acute inflammation induced by carrageenan in rats. It reduced the volume and number of leukocytes in inflammatory fluid in the granuloma air pouch model of chronic inflammation. It further decreased the levels of MDA in the fluid suggesting antioxidant property. JB elevated the concentrations of GSH in inflammatory exudates indicating free radical scavenging activity. It also significantly inhibited RBC lysis caused by hypotonic medium, suggesting membrane-stabilizing property.JB has in vivo anti-inflammatory activity, which may be related to its antioxidant and membrane-stabilizing properties, supporting its use for the treatment of arthritic disorder.

Design, Synthesis, Characterization and in silico molecular docking studies and in vivo anti-inflammatory Activity of Pyrazoline clubbed Thiazolinone Derivatives

2020 ◽  
Vol 17 ◽  
Author(s):  
Deepak Kumar Singh ◽  
Mayank Kulshreshtha ◽  
Yogesh Kumar ◽  
Pooja A Chawla ◽  
Akash Ved ◽  
...  
Keyword(s):  
Molecular Docking ◽  
Biological Activities ◽  
Inflammatory Activity ◽  
Standard Drug ◽  
Docking Score ◽  
Chemical Structures ◽  
Cox 2 ◽  
Anti Inflammatory ◽  
Cox 1

Background: The pyrazolines give the reactions of aliphatic derivatives, resembling unsaturated compounds in their behavior towards permanganate and nascent hydrogen. This nucleus has been associated with various biological activities including inflammatory. Thiazolinone is a heterocyclic compound that contains both sulfur and nitrogen atom with a carbonyl group in their structure.Thiazolinone and their derivatives have attracted continuing interest because of their various biological activities, such as anti-inflammatory, antimicrobial, anti-proliferative, antiviral, anticonvulsant etc. The aim of the research was to club pyrazoline nucleus with thiazolinone in order to have significantanti-inflammatory activity. The synthesized compounds were chemically characterized for the establishment of their chemical structures and to evaluate as anti-inflammatory agent. Method: In the present work, eight derivatives of substituted pyrazoline (PT1-PT8) were synthesized by a three step reaction.The compounds were subjected to spectral analysis by Infrared, Mass and Nuclear magnetic resonance spectroscopy and elemental analysis data. All the synthesized were evaluated for their in vivo anti-inflammatory activity. The synthesized derivatives were evaluated for their affinity towards target COX-1 and COX-2, using indomethacin as the reference compound molecular docking visualization through AutoDock Vina. Results: Compounds PT-1, PT-3, PT-4 and PT-8 exhibited significant anti-inflammatory activity at 3rd hour being 50.7%, 54.3%, 52.3% and 57% respectively closer to that of the standard drug indomethacin (61.9%).From selected anti-inflammatory targets, the synthesized derivatives exhibited better interaction with COX-1 and COX-2 receptor, where indomethacin showed docking score of -6.5 kJ/mol, compound PT-1 exhibited highest docking score of -9.1 kJ/mol for COX-1 and compound PT-8 having docking score of 9.4 kJ/mol for COX-2. Conclusion: It was concluded that synthesized derivatives have more interaction with COX-2 receptors in comparison to the COX-1 receptors because the docking score with COX-2 receptors were very good. It is concluded that the synthesized derivatives (PT-1 to PT-8) are potent COX-2 inhibitors.

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