Investigations of Anti-Inflammatory Activity of a Peptide-Based Hydrogel Using Rat Air Pouch Model

2018 ◽  
Vol 11 (3) ◽  
pp. 2849-2859 ◽  
Author(s):  
Pramod K. Gavel ◽  
Hamendra S. Parmar ◽  
Versha Tripathi ◽  
Narendra Kumar ◽  
Ankan Biswas ◽  
...  
Keyword(s):  
Inflammatory Activity ◽  
Air Pouch ◽  
Anti Inflammatory ◽  
Air Pouch Model

scholarly journals Suppressions of Serotonin-Induced Increased Vascular Permeability and Leukocyte Infiltration byBixa orellanaLeaf Extract

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Yoke Keong Yong ◽  
NurShahira Sulaiman ◽  
Muhammad Nazrul Hakim ◽  
Gwendoline Ee Cheng Lian ◽  
Zainul Amirudin Zakaria ◽  
...  
Keyword(s):  
Vascular Permeability ◽  
Inflammatory Activity ◽  
Blue Dye ◽  
Leukocyte Infiltration ◽  
Permeability Model ◽  
Air Pouch ◽  
Rat Paw Edema ◽  
Anti Inflammatory ◽  
Pharmacological Screening ◽  
Air Pouch Model

The aim of the present study was to evaluate the anti-inflammatory activities of aqueous extract ofBixa orellana(AEBO) leaves and its possible mechanisms in animal models. The anti-inflammatory activity of the extract was evaluated using serotonin-induced rat paw edema, increased peritoneal vascular permeability, and leukocyte infiltrations in an air-pouch model. Nitric oxide (NO), indicated by the sum of nitrites and nitrates, and vascular growth endothelial growth factor (VEGF) were measured in paw tissues of rats to determine their involvement in the regulation of increased permeability. Pretreatments with AEBO (50 and 150 mg kg−1) prior to serotonin inductions resulted in maximum inhibitions of 56.2% of paw volume, 45.7% of Evans blue dye leakage in the peritoneal vascular permeability model, and 83.9% of leukocyte infiltration in the air-pouch model. 57.2% maximum inhibition of NO and 27% of VEGF formations in rats’ paws were observed with AEBO at the dose of 150 mg kg−1. Pharmacological screening of the extract showed significant (P<0.05) anti-inflammatory activity, indicated by the suppressions of increased vascular permeability and leukocyte infiltration. The inhibitions of these inflammatory events are probably mediated via inhibition of NO and VEGF formation and release.

scholarly journals Anti-Inflammatory Activity of the Essential Oil Citral in Experimental Infection withStaphylococcus aureusin a Model Air Pouch

2017 ◽  
Vol 2017 ◽  
pp. 1-10 ◽  
Author(s):  
Hellen Braga Martins ◽  
Nathan das Neves Selis ◽  
Clarissa Leal Silva e Souza ◽  
Flávia S. Nascimento ◽  
Suzi Pacheco de Carvalho ◽  
...  
Keyword(s):  
Essential Oil ◽  
Blood Cells ◽  
Mutual Influence ◽  
White Blood Cells ◽  
Local Treatment ◽  
Inflammatory Activity ◽  
Air Pouch ◽  
Qpcr Array ◽  
Anti Inflammatory ◽  
Air Pouch Model

This study proposes to implement an alternative and effective strategy for local treatment of disease provoked byS. aureus. For the analysis of possible anti-inflammatory activity of essential oil, after establishing an air pouch model, 48 male mice of Balb/c were treated, infected, and euthanized at 4 and 8 h. Thus, the total and differential white blood cells were counted in the animal’s blood, and cytokines IL-1β, IL-6, and TNF-αwere titrated using ELISA in the air pouch lavage. Moreover, TNF-α, IL-1β, and IL-6 gene expression was analyzed through an RT-qPCR array, andS. aureuswas quantified using qPCR. Our results,p<0.05, showed that EOC reduced the quantity of microorganisms. The group of mice treated with essential oil citral showed a significant decrease in TNF-αlevels in tests demonstrating anti-inflammatory activity. There is no data about the mutual influence of the air pouch model, essential oil citral, andS. aureus. Thus, considering the interaction of these variables and the anti-inflammatory activity of the essential oil citral, we demonstrated, by alternative local treatment, a new antimicrobial agent that is not an antibiotic.

scholarly journals Evaluation of anti-inflammatory and anti-angiogenesis effects of naloxone in the rat air pouch model of inflammation

10.19082/7685 ◽  
2020 ◽  
Vol 12 (2) ◽  
pp. 7685-7693
Author(s):  
Tahereh Eteraf-Oskouei ◽  
Atefeh Zabiholahi ◽  
Moslem Najafi ◽  
Bohlool Habibi-Asl
Keyword(s):  
Air Pouch ◽  
Anti Inflammatory ◽  
Air Pouch Model

scholarly journals Aqueous extract from Ipomoea asarifolia (Convolvulaceae) leaves and its phenolic compounds have anti-inflammatory activity in murine models of edema, peritonitis and air-pouch inflammation

2016 ◽  
Vol 192 ◽  
pp. 225-235 ◽  
Author(s):  
Allanny A. Furtado ◽  
Manoela Torres-Rêgo ◽  
Maíra C.J.S. Lima ◽  
Mariana A.O. Bitencourt ◽  
Andréia Bergamo Estrela ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Aqueous Extract ◽  
Inflammatory Activity ◽  
Murine Models ◽  
Air Pouch ◽  
Anti Inflammatory ◽  
Ipomoea Asarifolia

Insulin Derived Fibrils Induce Cytotoxicity in vitro and Trigger Inflammation in Murine Models

2021 ◽  
pp. 193229682110338
Author(s):  
Brianne E. Lewis ◽  
Adam Mulka ◽  
Li Mao ◽  
Roshanak Sharafieh ◽  
Yi Qiao ◽  
...  
Keyword(s):  
Tissue Reaction ◽  
Inflammatory Activity ◽  
Dependent Diabetes Mellitus ◽  
Air Pouch ◽  
Tissue Reactions ◽  
And Function ◽  
Air Pouch Model ◽  
Insulin Fibrils

Background: Effective exogenous insulin delivery is the cornerstone of insulin dependent diabetes mellitus management. Recent literature indicates that commercial insulin-induced tissue reaction and cellular cytotoxicity may contribute to variability in blood glucose as well as permanent loss of injection or infusion site architecture and function. It is well accepted that insulin formulations are susceptible to mechanical and chemical stresses that lead to insulin fibril formation. This study aims to characterize in vitro and in vivo toxicity, as well as pro-inflammatory activity of insulin fibrils. Method: In vitro cell culture evaluated cytotoxicity and fibril uptake by macrophages and our modified murine air-pouch model quantified inflammatory activity. The latter employed FLOW cytometry and histopathology to characterize fibril-induced inflammation in vivo, which included fibril uptake by inflammatory phagocytes. Results: These studies demonstrated that insulin derived fibrils are cytotoxic to cells in vitro. Furthermore, inflammation is induced in the murine air-pouch model in vivo and in response, macrophages uptake fibrils both in vitro and in vivo. Conclusions: Administration of insulin fibrils can lead to cytotoxicity in macrophages. In vivo data demonstrate insulin fibrils to be pro-inflammatory which over time can lead to cumulative cell/tissue toxicity, inflammation, and destructive wound healing. Long term, these tissue reactions could contribute to loss of insulin injection site architecture and function.

scholarly journals Anti-inflammatory and anti-angiogenesis effect of bee pollen methanolic extract using air pouch model of inflammation

2020 ◽  
Vol 15 (1) ◽  
pp. 66
Author(s):  
Tahereh Eteraf-Oskouei ◽  
Ayda Shafiee-Khamneh ◽  
Fariba Heshmati-Afshar ◽  
Abbas Delazar
Keyword(s):  
Methanolic Extract ◽  
Air Pouch ◽  
Bee Pollen ◽  
Anti Inflammatory ◽  
Air Pouch Model

The Anti-Inflammatory Effect of Bee Venom Stimulation in a Mouse Air Pouch Model Is Mediated by Adrenal Medullary Activity

2003 ◽  
Vol 15 (1) ◽  
pp. 93-96 ◽  
Author(s):  
Y.-B. Kwon ◽  
H.-W. Kim ◽  
T.-W. Ham ◽  
S.-Y. Yoon ◽  
D.-H. Roh ◽  
...  
Keyword(s):  
Bee Venom ◽  
Air Pouch ◽  
Anti Inflammatory ◽  
Air Pouch Model ◽  
Inflammatory Effect
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