scholarly journals Selective Laser Efficiency of Green-Synthesized Silver Nanoparticles by Aloe arborescens and Its Wound Healing Activities in Normal Wounded and Diabetic Wounded Fibroblast Cells: In vitro Studies

2020 ◽  
Vol Volume 15 ◽  
pp. 6855-6870
Author(s):  
Sathish Sundar Dhilip Kumar ◽  
Nicolette Nadene Houreld ◽  
Heidi Abrahamse
Keyword(s):  
Wound Healing ◽  
Silver Nanoparticles ◽  
In Vitro Studies ◽  
Fibroblast Cells ◽  
Laser Efficiency ◽  
Aloe Arborescens ◽  
Green Synthesized Silver Nanoparticles

In Vitro Evaluation of Anti-Mitotic Activity of Green Synthesized Silver Nanoparticles from Ethanolic Extract of Chromolaena odorata, Caesalpinia coriaria (Bark and Leaves) using Allium cepa Roots

2021 ◽  
pp. 4107-4110
Author(s):  
Nishita Gogia ◽  
Anandhi D ◽  
Kanaga G ◽  
Revathi K
Keyword(s):  
Silver Nanoparticles ◽  
Mitotic Activity ◽  
Mitotic Index ◽  
Allium Cepa ◽  
Chromolaena Odorata ◽  
Root Tips ◽  
Antimitotic Activity ◽  
Biological Potential ◽  
Green Synthesized Silver Nanoparticles

The scope of the present study was to evaluate the In-vitro biological potential of Green synthesized silver nanoparticles from Chromolaena odorata, Caesalpinia coriaria (Bark) and Caesalpinia coriaria (Leaf). We investigated the effect of green synthesized silver nanoparticles from Chromolaena odorata, Caesalpinia coriaria (Bark) and Caesalpinia coriaria (Leaf) by studying anti-mitotic activity An attempt has been made to evaluate the anti-mitotic activity of silver nanoparticles using Allium cepa roots. The mitotic index of the root tips meristem was calculated and compared with the standard methotrexate. Antimitotic activity results shows the mitotic index was ranged from 30-40%. Green synthesized silver nanoparticles from Chromolaena odorato, Caesalpinia coriaria (Bark) and Caesalpinia coriaria (Leaf) possess significant anti-mitotic activity.

scholarly journals Effect of platelet-rich fibrin on cell proliferation, migration, differentiation, inflammation, and osteoclastogenesis: a systematic review of in vitro studies

2019 ◽  
Vol 24 (2) ◽  
pp. 569-584 ◽  
Author(s):  
Franz-Josef Strauss ◽  
Jila Nasirzade ◽  
Zahra Kargarpoor ◽  
Alexandra Stähli ◽  
Reinhard Gruber
Keyword(s):  
Systematic Review ◽  
Cell Proliferation ◽  
Wound Healing ◽  
Bone Regeneration ◽  
Therapeutic Potential ◽  
In Vitro Studies ◽  
Bioactive Molecules ◽  
Platelet Rich Fibrin ◽  
Regenerative Dentistry

Abstract Objective To systematically assess the effects of platelet-rich fibrin (PRF) on in vitro cellular behavior. Methods A systematic electronic search using MEDLINE database was performed. In vitro studies using PRF were considered and articles published up to June 31, 2018 were screened. Eligible studies were selected based on the use of human PRF. Results In total, 1746 titles were identified with the search terms, from these 37 met the inclusion criteria and were chosen for data extraction. In addition, 16 new studies, mainly published in 2019, were also included in the analysis resulting in 53 studies. No meta-analysis could be performed due to the heterogeneity of study designs. Included studies show that PRF enhances proliferation, migration, adhesion, and osteogenic differentiation on a variety of cell types along with cell signaling activation. Furthermore, PRF reduces inflammation, suppresses osteoclastogenesis, and increases the expression of various growth factors in mesenchymal cells. Summary and conclusions Despite some notable differences of the studies, the overall findings suggest a positive effect of PRF on cell proliferation, migration, adhesion, differentiation, and inflammation pointing towards a therapeutic potential in regenerative dentistry. Clinical relevance PRF serves as a reservoir of bioactive molecules to support wound healing and bone regeneration. Although the cellular mechanisms by which PRF supports the clinical outcomes remain unclear, in vitro research provides possible explanations. This systematic review aims to provide an update of the existing research on how PRF affects basic physiological processes in vitro. The overall findings suggest that PRF induces cell proliferation, migration, adhesion, and differentiation along with possessing anti-inflammatory properties further supporting its therapeutic potential in wound healing and bone regeneration.

Wound healing potential of green synthesized silver nanoparticles prepared from Lansium domesticum fruit peel extract

2015 ◽  
Vol 5 (2) ◽  
pp. 159-164 ◽  
Author(s):  
Shiv Shankar ◽  
Lily Jaiswal ◽  
Rangabhatla Sai Laxmi Aparna ◽  
Rangabhatla GunneswaraS V Prasad ◽  
Govindappa Prem Kumar ◽  
...  
Keyword(s):  
Wound Healing ◽  
Silver Nanoparticles ◽  
Fruit Peel ◽  
Green Synthesized Silver Nanoparticles ◽  
Lansium Domesticum ◽  
Peel Extract

scholarly journals Synthesis and biochemical characterization of silver nanoparticles grafted chitosan (Chi-Ag-NPs): in vitro studies on antioxidant and antibacterial applications

2020 ◽  
Vol 2 (4) ◽  
Author(s):  
Pavan Kumar Dara ◽  
R. Mahadevan ◽  
P. A. Digita ◽  
S. Visnuvinayagam ◽  
Lekshmi R. G. Kumar ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Biochemical Characterization ◽  
In Vitro Studies ◽  
Ag Nps

scholarly journals Skin Regeneration Using Dermal Substrates that Contain Autologous Cells and Silver Nanoparticles to Promote Antibacterial Activity: In Vitro Studies

2017 ◽  
Vol 182 (S1) ◽  
pp. 376-382 ◽  
Author(s):  
Michael A. J. Zieger ◽  
Manuel Ochoa ◽  
Rahim Rahimi ◽  
Gonzalo Campana ◽  
Sunil Tholpady ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Skin Regeneration ◽  
In Vitro Studies

Low concentrations of silver nanoparticles have a beneficial effect on wound healing in vitro

2017 ◽  
Vol 19 (3) ◽  
Author(s):  
Nikola Ambrožová ◽  
Bohumil Zálešák ◽  
Jitka Ulrichová ◽  
Kateřina Čížková ◽  
Adéla Galandáková
Keyword(s):  
Wound Healing ◽  
Silver Nanoparticles ◽  
Beneficial Effect ◽  
Low Concentrations

scholarly journals Evaluation of in vitro fibroblast migration by electrospun triple-layered PU-CA/gelatin.PRGF/PU-CA scaffold using an AAVS1 targeted EGFP reporter cell line

Bioimpacts ◽  
2021 ◽  
Author(s):  
Forough Shams ◽  
Hamideh Moravvej ◽  
Simzar Hosseinzadeh ◽  
Bahram Kazemi ◽  
Masoumrh Rajabibazl ◽  
...  
Keyword(s):  
Wound Healing ◽  
Real Time ◽  
Reporter Gene ◽  
Healing Process ◽  
Functional Evaluation ◽  
Fibroblast Cells ◽  
Fibroblast Migration ◽  
Egfp Reporter ◽  
And Migration

Introduction: Migration of fibroblast cells in wound areas is a critical aspect of the wound healing process. Employment of enhanced green fluorescent protein (EGFP) labeled fibroblast cells facilitates real-time monitoring and functional evaluation of these cells in both in vitro and in vivo settings. Plasma rich in growth factor (PRGF) is a potent accelerator of wound healing; therefore, in this study, a novel method to fabricate an electrospun bioactive scaffold containing PRGF was employed to induce in vitro cell proliferation and migration. Methods: First, the EGFP reporter gene was integrated into the AAVS1 locus of fibroblast cells using CRISPR/Cas9 system. Then, PRGF was obtained from platelet-rich plasma, and a multi-layered scaffold was fabricated using polyurethane-cellulose acetate (PU-CA) fibers as the outer layers and PRGF-containing gelatin fibers were located in the internal layer like a central strip. Scanning electron microscopy (SEM), tensile, water contact angle, and FTIR tests were performed to assess the characteristics of the scaffolds. The EGFP targeted cells were cultured on scaffolds with or without PRGF to investigate their viability, toxicity, and migration pattern in response to the release profile. Results: Fluorescence images showed that the number of migrating cells on scaffold containing PRGF was more significant than PU-CA scaffold up to day 6. Increased expression of SGPL1, DDR2, and VEGF genes was also observed on the scaffold containing PRGF compared to PU-CA using real-time polymerase chain reaction (PCR) analysis with around 3-, 2-, and 2-fold enhancement, respectively. Conclusion: The current scaffold provides the appropriate template for cell attachment and migration. In addition, the present results highlight the potential of reporter gene targeting for the in vitro analysis of biological processes such as migration.

scholarly journals Development of Antimicrobial Conjugates and Evaluating its Antibacterial potential and Wound Healing ability

2021 ◽  
Vol 23 (09) ◽  
pp. 540-555
Author(s):  
Deepak Tom Jose ◽  
◽  
Sivagurunathan, P ◽  
Aswini, B ◽  
Dinesh, MD ◽  
...  
Keyword(s):  
Wound Healing ◽  
Wound Dressing ◽  
Fibroblast Cell ◽  
Fibroblast Cells ◽  
L929 Fibroblast ◽  
Microscopic Studies ◽  
Engineered Materials ◽  
Cell Migration And Proliferation ◽  
Antibacterial Potential

Antimicrobial peptides from Streptomyces sp. and marine fish (Carangoides malabaricus) were extracted and developed as conjugates in the present study. The objective was framed to analyze the ability of conjugate to retard the growth of test bacteria causing diabetic foot ulcers. Fibroblast cell adhesion on AMP conjugates coated mesh samples were recorded using microscopic studies with an aim of developing a novel tissue engineered wound dressing material. Thus developed tissue engineered materials were evaluated for its antibacterial potential against wound pathogens; and to assay the wound healing ability using a standard in vitro wound scratch method. Tissue engineered materials were developed using L929 fibroblast cells. L929 fibroblast cells attachment and its stage wise development on wound dressing mesh materials were microscopically observed. In vitro wound healing assay revealed that the developed conjugates (containing AMPs) exhibited cell migration and proliferation after 12th hour of incubation indicating the wound healing abilities. The results showed that the developed tissue engineered wound dressing material has commercial interest in near future.

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