Wound healing potential of green synthesized silver nanoparticles prepared from Lansium domesticum fruit peel extract

2015 ◽  
Vol 5 (2) ◽  
pp. 159-164 ◽  
Author(s):  
Shiv Shankar ◽  
Lily Jaiswal ◽  
Rangabhatla Sai Laxmi Aparna ◽  
Rangabhatla GunneswaraS V Prasad ◽  
Govindappa Prem Kumar ◽  
...  
Keyword(s):  
Wound Healing ◽  
Silver Nanoparticles ◽  
Fruit Peel ◽  
Green Synthesized Silver Nanoparticles ◽  
Lansium Domesticum ◽  
Peel Extract

Biogenic synthesis of nano-sulfur using Punica granatum fruit peel extract with enhanced antimicrobial activities for accelerating wound healing

Nano Futures ◽  
2021 ◽  
Vol 5 (4) ◽  
pp. 045003
Author(s):  
K Samrat ◽  
M N Chandraprabha ◽  
R Hari Krishna ◽  
R Sharath ◽  
B G Harish
Keyword(s):  
Wound Healing ◽  
Granulation Tissue ◽  
Breaking Strength ◽  
Punica Granatum ◽  
Control Group ◽  
Fruit Peel ◽  
Burn Wound ◽  
Excision Wound ◽  
Tissue Weight ◽  
Peel Extract

Abstract Microbial wound infections leading to secondary complications in wound healing has resulted in high demand for therapeutic drugs with improved efficacy. Despite achieving enhanced bio-activity and higher bioavailability compared to its bulk form, nano-sulfur (SNP) has been explored to a very limited extent for wound healing applications. In this work, we prepare biogenic SNP (SNP-B) via simple biogenic technique using pomegranate (Punica granatum) peel extract and demonstrate its antimicrobial and wound healing activity. The SNP-B was characterized using powder x-ray diffractometer, FESEM, transmission electron microscopy and Raman spectroscopy. Different wound models (excision, incision, dead space and burn) were used to assess the wound healing potential of SNP-B. The 2% (w/w) SNP-B treated group exhibited enhanced wound contraction rate (excision wound, 99.62 ± 0.59%; burn wound, 99.46 ± 0.59%), breaking strength (393.2 ± 10.87 g cm−2), and granulation tissue weight (166.8 ± 9.45 mg) compared to the control group (excision wound, 84.24 ± 2.78%; burn wound, 90.58 ± 3.2%; breaking strength, 241.3 ± 16.11 g cm−2; granulation tissue weight, 91.17 ± 7.28 mg). The efficacy of 2% (w/w) SNP-B was comparable to that of standard (5% w/w povidone-iodine ointment) in all the wound models analyzed. The SNP-B showed enhanced antibacterial activity with a MIC value of 90, 80, 80, and 60 μg ml−1 for Pseudomonas aeruginosa, Escherichia coli, Bacillus subtilis and Staphylococcus aureus, respectively. The results obtained prove the potential of SNP-B as a multifunctional therapeutic agent for topical applications.

scholarly journals Green Synthesized Silver Nanoparticles Using Tridax Procumbens for Topical Application: Excision Wound Model and Histopathological Studies

Pharmaceutics ◽  
2021 ◽  
Vol 13 (11) ◽  
pp. 1754
Author(s):  
Farhat Fatima ◽  
Mohammed F. Aldawsari ◽  
Mohammed Muqtader Ahmed ◽  
Md. Khalid Anwer ◽  
Maimuna Naz ◽  
...  
Keyword(s):  
Wound Healing ◽  
Silver Nanoparticles ◽  
Particle Analysis ◽  
Histopathological Study ◽  
Treated Animal ◽  
Excision Wound ◽  
Wound Model ◽  
Tridax Procumbens ◽  
Bet Analysis ◽  
Green Synthesized Silver Nanoparticles

The objective of this study was to synthesize silver nanoparticles from the leaves of Tridax procumbens and develop its topical gels using chitosan to investigate the wound healing efficacy concomitant with the histopathological study. Green synthesized silver nanoparticles (AgNPs) were prepared by reacting silver nitrate (0.3 M) with leaf extract and characterized by particle analysis, FTIR, XRD, SEM, BET, and TGA. The results revealed formed AgNPs were nano-sized (138 ± 2.1 nm), monodispersed (PDI: 0.460 ± 0.3), inter-particle repulsion (zeta: −20.4 ± 5.20 mV), stabilized, crystalline and, spherical with size ranging from 80–100 nm as per SEM micro photos. The BET analysis of AgNPs presents the surface area (12.861 m2/g), pore volume (0.037 cc/g), and pore radius (24.50 nm).TGA results show a loss of 13.39% up to 300 °C. The topical formulation was developed by loading AgNPs in chitosan-based gels, evaluated by pH, thermal cycling, centrifugal, and spreadability tests. AgNPs chitosan gels results showed skin compatibility, higher stability, and spreading ability. The maximum antibacterial zone of inhibition was found to be 25 ± 0.98 mm for bacillus subtitles and 30 ± 1.99 mm for Klebsiella pneumoniae, respectively. Nanosilver-containing gel also showed excellent compatibility with erythrocytes. Excision wound model was used to assess the wound healing property of the developed AgNP gels, the results of which indicated a significantly progressive healing process in test-group of animals treated with chitosan-based gels containing AgNPs. A histopathological study further confirmed the almost normal skin structure of treated animal tissue compared to standard and negative control. Thus, green synthesized AgNPs loaded chitosan-based topical gel can potentially be used for wound healing application.

Green synthesized silver nanoparticles-impregnated novel gum kondagogu–chitosan biosheet for tissue engineering and wound healing applications

2021 ◽  
Author(s):  
Hanumant Singh Rathore ◽  
Uma Tiruchirapalli Sivagnanam ◽  
L. Stanley Abraham ◽  
D. Prakash ◽  
Ramesh C. Panda ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Wound Healing ◽  
Silver Nanoparticles ◽  
Gum Kondagogu ◽  
Green Synthesized Silver Nanoparticles

scholarly journals Green and Chemical Silver Nanoparticles and Pomegranate Formulations to Heal Infected Wounds in Diabetic Rats

Antibiotics ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 1343
Author(s):  
Renan Aparecido Fernandes Scappaticci ◽  
Andresa Aparecida Berretta ◽  
Elina Cassia Torres ◽  
Andrei Felipe Moreira Buszinski ◽  
Gabriela Lopes Fernandes ◽  
...  
Keyword(s):  
Wound Healing ◽  
Silver Nanoparticles ◽  
Inflammatory Infiltrate ◽  
Diabetic Rats ◽  
Pomegranate Peel ◽  
Considerable Potential ◽  
Infected Wounds ◽  
Pomegranate Peel Extract ◽  
Peel Extract

Infected cutaneous ulcers from diabetic rats with Candida albicans and Streptococcus aureus were treated with spray formulations containing green silver nanoparticles (GS), chemical silver nanoparticles (CS), or pomegranate peel extract (PS). After wound development and infection, the treatments were performed twice per day for 14 days. The wound healing was analyzed on days 2, 7, and 14 through the determination of CFUs, inflammatory infiltrate, angiogenesis, fibroplasia, myeloperoxidase, and collagen determination. Expressive improvement in wound healing was noted using both silver nanoparticles for 7 days. All the treatments were superior to controls and promoted significant S. aureus reduction after 14 days. CS presented better anti-inflammatory results, and GS and CS the highest number of fibroblasts. Despite the techniques’ limitations, GS and CS demonstrated considerable potential for managing infected wounds, especially considering no early strategies prior to the drugs, such as the debridement of these wounds, were included.

Helminthicidal and Larvicidal Potentials of Biogenic Silver Nanoparticles Synthesized from Medicinal Plant Momordica charantia

2019 ◽  
Vol 15 (7) ◽  
pp. 781-789 ◽  
Author(s):  
Amruta Shelar ◽  
Jaiprakash Sangshetti ◽  
Shampa Chakraborti ◽  
Ajay Vikram Singh ◽  
Rajendra Patil ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Larvicidal Activity ◽  
Visible Region ◽  
Surface Reactivity ◽  
Momordica Charantia ◽  
Morphological Alteration ◽  
Face Centered Cubic ◽  
Fruit Peel ◽  
Vis Spectroscopy ◽  
Peel Extract

Background: The drug formulations used to control mosquito vectors and helminth infections have resulted in the development of resistance, and negative impact on non-target organisms and environment. Objective: Plant-mediated synthesis of silver nanoparticles (P-AgNPs) using aqueous fruit peel extract of M. charantia, applications of P-AgNPs for helminthicidal activity against Indian earthworms (P. posthuma) and larvicidal activity against larvae of mosquito A. albopictus and A. aegypti. Methods: Aqueous fruit peel extract of Momordica charantia was used to reduce silver ions to silver nanoparticles (P-AgNPs). UV-Visible (UV-Vis) Spectroscopy, X-ray diffraction, Fourier Transform Infrared Spectroscopy and Transmission Electron Microscopy characterize synthesized P-AgNPs. The motility and survival rate of the worms were recorded for the helminthicidal activity. Percent mortality of larvae of A. albopictus and A. aegypti was recorded for larvicidal activity. Results: The UV-Vis absorption spectrum of P-AgNPs showed a strong surface plasmon absorption band in the visible region with a maximum absorption at 445 nm indicating the synthesis of silver nanoparticles by the addition of aqueous fruit peel extract. The XRD spectrum of P-AgNPs showed Bragg's reflection peaks 2θ value characteristics for the Face-Centered Cubic (FCC) structure of silver. The sharp absorption peak in FTIR at 1659 cm-1 assigned to C=O stretching vibration in carbonyl compounds represents terpenoids, flavonoids and polyphenols in the corona of PAgNPs; a 2 mg/mL of P-AgNPs. The concentration aqueous extract and P-AgNPs showed complete death of worms (the morphological alteration/coiling of body). A 20 ppm concentration of PAgNPs showed 85% mortality in larvae of Ae. albopictus and Ae. aegypti. P-AgNPs were nontoxic at low concentrations. Conclusion: The aqueous extracts played a dual role as reducing and capping agent during the biosynthesis of AgNPs as per FTIR and XRD results. The surface reactivity facilitated by biomolecule corona attached to silver nanoparticles can further help to functionalize AgNPs in various pharmaceuticals, biomedicals, and environmental applications.

scholarly journals POTENSI EKSTRAK KULIT JERUK KUNCI (Citrus microcarpa Bunge) SEBAGAI BIOREDUKTOR DALAM SINTESIS NANOPARTIKEL PERAK

2021 ◽  
Vol 7 (1) ◽  
pp. 12-20
Author(s):  
Athiah Masykuroh ◽  
Nadia Nia Nurulita
Keyword(s):  
Silver Nanoparticles ◽  
Surface Plasmon Resonance ◽  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Fruit Peel ◽  
Color Test ◽  
Agno3 Solution ◽  
Average Size ◽  
Peel Extract ◽  
Scanning Electron

Nowadays silver nanoparticles (AgNPs) synthesized so often by plant extracts as a reductor. The synthesis of AgNPs was carried out by Citrus microcarpa Bunge fruit peel extract a reductor in various extract concentrations (10, 15, and 20%), concentration of AgNO3 solution of 0.15M and temperature of 700C. The presence of AgNPs was determined by color test and the formation of Surface Plasmon Resonance (SPR) using UV-Vis Spectrophotometer while to determine the morphology and size of the nanoparticles using Scanning Electron  Microscope (SEM). The results of the analysis showed that AgNPs was formed at colloidal phase with dark brown color with wavelengths of 457.30 nm, 478.90 nm, and 422.80 nm for variation concentration of 10, 15 and 20% with slightly spherical, slightly elongated and jagged morphology with average size of 253.8 nm (10%), 254.2 nm (15%) and 253.9 nm (20%).

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