scholarly journals Various factors affect lipopolysaccharide sensitization in cell cultures

BioTechniques ◽  
2020 ◽  
Vol 69 (2) ◽  
pp. 126-132
Author(s):  
Nan Yang ◽  
Don D Sin ◽  
Delbert R Dorscheid
Keyword(s):  
Cell Lines ◽  
Cell Cultures ◽  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
Signal Loss ◽  
The Media ◽  
Fetal Bovine

Commercially available lipopolysaccharide (LPS) is commonly used in research. Although protocols for its use are well established, we experienced a loss of LPS responsiveness in our cell cultures despite no obvious experimental changes. Our cell lines were stimulated with LPS and the media quantified for LPS responsiveness via an IL-8 ELISA. We discovered that the major cause of signal loss was differences in fetal bovine serum (FBS) formulation and concentration. One FBS formulation was notably better at eliciting an IL-8 signal than the second FBS, and 10% FBS in media was better at inducing LPS responsiveness than lower concentrations. We urge researchers to be aware of inherent variations in seemingly commonplace reagents as they may be unexpected sources of inconsistencies.

Platelet-Rich Plasma Can Replace Fetal Bovine Serum in Human Meniscus Cell Cultures

2013 ◽  
Vol 19 (11) ◽  
pp. 892-899 ◽  
Author(s):  
Veronica K. Gonzales ◽  
Eric L.W. de Mulder ◽  
Trix de Boer ◽  
Gerjon Hannink ◽  
Tony G. van Tienen ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
Platelet Rich Plasma ◽  
Meniscus Cell ◽  
Fetal Bovine

scholarly journals Evaluation of the major royal jelly proteins as an alternative to fetal bovine serum in culturing human cell lines

2016 ◽  
Vol 17 (6) ◽  
pp. 476-483 ◽  
Author(s):  
Di Chen ◽  
Xiao-xuan Xin ◽  
Hao-cheng Qian ◽  
Zhang-yin Yu ◽  
Li-rong Shen
Keyword(s):  
Cell Lines ◽  
Human Cell ◽  
Bovine Serum ◽  
Royal Jelly ◽  
Fetal Bovine Serum ◽  
Human Cell Lines ◽  
Fetal Bovine

Effect of fetal bovine serum on the growth and survival of insect cell cultures

1970 ◽  
Vol 48 (3) ◽  
pp. 427-432 ◽  
Author(s):  
S. S. Sohi ◽  
Cheryl Smith
Keyword(s):  
Adverse Effect ◽  
Cell Lines ◽  
Insect Cell ◽  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
Maximum Growth ◽  
Growth And Survival ◽  
Fetal Bovine ◽  
High Concentrations ◽  
Insect Cell Lines

The effect of different concentrations of fetal bovine serum (FBS) on the growth and survival of three insect cell lines—Aedes aegypti, Antheraea eucalypti, and Bombyx mori—was studied in a series of 10 experiments. Cell viability was low and no growth occurred in any of the cell lines when FBS was omitted from the medium. Maximum growth of A. aegypti cells was obtained with 10% FBS. There was no further increase in this growth when FBS was increased to 20 and 30%, and neither did the increased concentrations have any appreciable adverse effect on the growth or survival of these cells. Maximum growth of A. eucalypti and B. mori cells was obtained in 5% FBS; the growth of these cells was significantly less in 20 and 30% FBS. Viability of A. eucalypti cells was quite low in 20 and 30% FBS. There was, however, no adverse effect on the viability of B. mori cells at these high concentrations.

HUMAN SERUM AS AN ECONOMICAL SUBSTITUTE FOR FETAL BOVINE SERUM IN LYMPHOID CELL CULTURES

1974 ◽  
Vol 18 (1) ◽  
pp. 86 ◽  
Author(s):  
J. LESLIE GLICK ◽  
CLARA LOCKWOOD ◽  
JETHON WILLIAMS ◽  
BEN W. PAPERMASTER ◽  
ALEX A. BURNS ◽  
...  
Keyword(s):  
Human Serum ◽  
Cell Cultures ◽  
Lymphoid Cell ◽  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
Fetal Bovine

Characterization of osteoblastic properties of 7F2 and UMR-106 cultures after acclimation to reduced levels of fetal bovine serum

2008 ◽  
Vol 86 (7) ◽  
pp. 403-415 ◽  
Author(s):  
S. Ganguly ◽  
L.A. Ashley ◽  
C.M. Pendleton ◽  
R.D. Grey ◽  
G.C. Howard ◽  
...  
Keyword(s):  
Cell Lines ◽  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
Phenol Red ◽  
Ph Indicator ◽  
Fetal Bovine ◽  
Experimental Findings ◽  
Umr 106 ◽  
Culture Protocol

Estrogen plays an important role in skeletal physiology by maintaining a remodeling balance between the activity of osteoblasts and osteoclasts. In an attempt to decipher the mechanism through which estrogen elicits its action on osteoblasts, experimentation necessitated the development of a culturing environment reduced in estrogenic compounds. The selected medium (OPTI-MEM) is enriched to sustain cultures under reduced fetal bovine serum (FBS) conditions and is devoid of the pH indicator phenol red, a suspected estrogenic agent. This protocol reduced the concentration of FBS supplementation to 0% through successive 24 h incubations with diminishing amounts of total FBS (1%, 0.1%, and 0%). The protocol does not appear to alter the viability, cell morphology, or osteoblast-like phenotype of 7F2 and UMR-106 cell lines when compared with control cells grown in various concentrations of FBS. Although the rate of mitotic divisions declined, the 7F2 and UMR-106 cultures continued to express osteoblast-specific markers and exhibited estrogen responsiveness. These experimental findings demonstrate that the culture protocol developed did not alter the osteoblast nature of the cell lines and provides a model system to study estrogen’s antiresorptive role on skeletal turnover.

scholarly journals Effects of Fetal Bovine Serum deprivation in cell cultures on the production of Anticarsia gemmatalis Multinucleopolyhedrovirus

2010 ◽  
Vol 10 (1) ◽  
pp. 68 ◽  
Author(s):  
Diego L Mengual Gómez ◽  
Mariano N Belaich ◽  
Vanina A Rodríguez ◽  
Pablo D Ghiringhelli
Keyword(s):  
Cell Cultures ◽  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
Serum Deprivation ◽  
Anticarsia Gemmatalis ◽  
Fetal Bovine

scholarly journals Methods of obtaining multipotent stem cells of the placenta in rats

2015 ◽  
Vol 70 (2) ◽  
pp. 73-77
Author(s):  
G. Svitina ◽  
L. Garmanchuk ◽  
V. Shabliy
Keyword(s):  
Stem Cells ◽  
Cell Therapy ◽  
Cell Cultures ◽  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
Enzymatic Digestion ◽  
Optimal Method ◽  
Rat Serum ◽  
Multipotent Stem Cells ◽  
Fetal Bovine

Placenta is a valuable source of multipotent stem cells (PMSC) widely used for cell therapy. Hence, the most optimal method of PMSC obtaining remains questionable. By methods of explant culturing and enzymatic digestion were obtained PMSC of fetal origin and multipotent features at 4 th passage. Allogeneic rat serum is not favor PMSC establishment and growth, consequently the use is made of fetal bovine serum, that is xenogeneic for cell cultures.

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