scholarly journals The choice of fetal bovine serum influences the degree of spontaneous mineralization on silk fibroin scaffolds in 3D cell cultures

2013 ◽  
Author(s):  
Samantha Paulsen ◽  
Jolanda Vetsch ◽  
Ralph Muller ◽  
Sandra Hofmann
Keyword(s):  
Silk Fibroin ◽  
Cell Cultures ◽  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
3D Cell Cultures ◽  
Fetal Bovine

scholarly journals Various factors affect lipopolysaccharide sensitization in cell cultures

BioTechniques ◽  
2020 ◽  
Vol 69 (2) ◽  
pp. 126-132
Author(s):  
Nan Yang ◽  
Don D Sin ◽  
Delbert R Dorscheid
Keyword(s):  
Cell Lines ◽  
Cell Cultures ◽  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
Signal Loss ◽  
The Media ◽  
Fetal Bovine

Commercially available lipopolysaccharide (LPS) is commonly used in research. Although protocols for its use are well established, we experienced a loss of LPS responsiveness in our cell cultures despite no obvious experimental changes. Our cell lines were stimulated with LPS and the media quantified for LPS responsiveness via an IL-8 ELISA. We discovered that the major cause of signal loss was differences in fetal bovine serum (FBS) formulation and concentration. One FBS formulation was notably better at eliciting an IL-8 signal than the second FBS, and 10% FBS in media was better at inducing LPS responsiveness than lower concentrations. We urge researchers to be aware of inherent variations in seemingly commonplace reagents as they may be unexpected sources of inconsistencies.

Platelet-Rich Plasma Can Replace Fetal Bovine Serum in Human Meniscus Cell Cultures

2013 ◽  
Vol 19 (11) ◽  
pp. 892-899 ◽  
Author(s):  
Veronica K. Gonzales ◽  
Eric L.W. de Mulder ◽  
Trix de Boer ◽  
Gerjon Hannink ◽  
Tony G. van Tienen ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
Platelet Rich Plasma ◽  
Meniscus Cell ◽  
Fetal Bovine

HUMAN SERUM AS AN ECONOMICAL SUBSTITUTE FOR FETAL BOVINE SERUM IN LYMPHOID CELL CULTURES

1974 ◽  
Vol 18 (1) ◽  
pp. 86 ◽  
Author(s):  
J. LESLIE GLICK ◽  
CLARA LOCKWOOD ◽  
JETHON WILLIAMS ◽  
BEN W. PAPERMASTER ◽  
ALEX A. BURNS ◽  
...  
Keyword(s):  
Human Serum ◽  
Cell Cultures ◽  
Lymphoid Cell ◽  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
Fetal Bovine

scholarly journals Effects of Fetal Bovine Serum deprivation in cell cultures on the production of Anticarsia gemmatalis Multinucleopolyhedrovirus

2010 ◽  
Vol 10 (1) ◽  
pp. 68 ◽  
Author(s):  
Diego L Mengual Gómez ◽  
Mariano N Belaich ◽  
Vanina A Rodríguez ◽  
Pablo D Ghiringhelli
Keyword(s):  
Cell Cultures ◽  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
Serum Deprivation ◽  
Anticarsia Gemmatalis ◽  
Fetal Bovine

scholarly journals Methods of obtaining multipotent stem cells of the placenta in rats

2015 ◽  
Vol 70 (2) ◽  
pp. 73-77
Author(s):  
G. Svitina ◽  
L. Garmanchuk ◽  
V. Shabliy
Keyword(s):  
Stem Cells ◽  
Cell Therapy ◽  
Cell Cultures ◽  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
Enzymatic Digestion ◽  
Optimal Method ◽  
Rat Serum ◽  
Multipotent Stem Cells ◽  
Fetal Bovine

Placenta is a valuable source of multipotent stem cells (PMSC) widely used for cell therapy. Hence, the most optimal method of PMSC obtaining remains questionable. By methods of explant culturing and enzymatic digestion were obtained PMSC of fetal origin and multipotent features at 4 th passage. Allogeneic rat serum is not favor PMSC establishment and growth, consequently the use is made of fetal bovine serum, that is xenogeneic for cell cultures.

Serum-converted platelet lysate can substitute for fetal bovine serum in human mesenchymal stromal cell cultures

Cytotherapy ◽  
2013 ◽  
Vol 15 (12) ◽  
pp. 1458-1468 ◽  
Author(s):  
Mariluz P. Mojica-Henshaw ◽  
Pam jacobson ◽  
Julie Morris ◽  
Linda Kelley ◽  
Jan Pierce ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Bovine Serum ◽  
Mesenchymal Stromal Cell ◽  
Stromal Cell ◽  
Fetal Bovine Serum ◽  
Platelet Lysate ◽  
Fetal Bovine

Effect of fetal bovine serum on mineralization in silk fibroin scaffolds

2015 ◽  
Vol 13 ◽  
pp. 277-285 ◽  
Author(s):  
Jolanda R. Vetsch ◽  
Samantha J. Paulsen ◽  
Ralph Müller ◽  
Sandra Hofmann
Keyword(s):  
Silk Fibroin ◽  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
Fetal Bovine

scholarly journals KONDROGENESIS ADIPOSE-DERIVED STEM CELLS MENGGUNAKAN PLATELET-RICH PLASMA PADA SCAFFOLD SUTRA

2020 ◽  
Vol 13 (1) ◽  
pp. 31-38
Author(s):  
Imam Rosadi ◽  
Karina Karina ◽  
Komang A. Wahyuningsih ◽  
Iis Rosliana ◽  
Tias Widyastuti ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bombyx Mori ◽  
Silk Fibroin ◽  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
Platelet Rich Plasma ◽  
Adipose Derived Stem Cells ◽  
Silk Fibroin Scaffold ◽  
Salt Leaching ◽  
Fetal Bovine

AbstrakStudi tentang kemampuan adipose-derived stem cells (ADSCs) sebagai sel punca yang dapat berdiferensiasi menjadi kondrosit menggunakan platelet-rich plasma (PRP) sebagai subtitusi fetal bovine serum (FBS) telah banyak dilaporkan. Penggunaan medium pertumbuhan dengan kombinasi ADSCs, PRP dan scaffold sutra masih belum banyak dipelajari dalam rekayasa jaringan kartilago. Studi ini bertujuan untuk mempelajari pengaruh medium yang mengandung 5%, 10% dan 20% PRP terhadap proses kondrogenesis ADSCs manusia yang dikultur pada scaffold sutra Bombyx mori Indonesia. Metode penelitian diawali dengan pembuatan scaffold sutra menggunakan metode salt-leaching, isolasi dan kultur ADSCs manusia dari jaringan lemak, uji pertumbuhan ADSCs pada scaffold sutra dengan variasi konsentrasi PRP pada medium serta analisis kadar glikosaminoglikan (GAG). Hasil penelitian menunjukkan bahwa ADSCs yang dikultur menggunakan PRP lebih tinggi laju pertumbuhannya dibandingkan dikultur menggunakan FBS selama 7 hari pengamatan. Kadar GAG yang disekresikan ADSCs kelompok PRP juga lebih tinggi dibandingkan kelompok FBS. Kadar GAG tertinggi pada hari ke-21 pengamatan adalah medium yang mengandung 20% PRP kemudian 10% dan 5%, sedangkan kadar GAG kelompok kontrol cenderung stabil pada kadar yang rendah. Berdasarkan hasil tersebut, medium yang mengandung PRP memiliki potensi dalam menginduksi kondrogenesis ADSCs yang dikultur pada scaffold sutra.Abstract The studies on adipose-derived stem cells (ADSCs) differentiation into chondrocytes using platelet-rich plasma (PRP) as a substitute for fetal bovine serum (FBS) have been reported. However, the combination of ADSCs, PRP and silk fibroin scaffold has not been widely studied for developing cartilage engineering. Therefore, this research aims to study the effect of medium containing 5%, 10% and 20% PRP towards chondrogenesis of human ADSCs cultured on silk fibroin scaffold from Indonesia Bombyx mori. At first, the silk fibroin scaffold was fabricated using a salt-leaching method, then ADSCs were isolated and cultured from adipose tissues. The assays of growth curve and biocompatibility of silk fibroin scaffold toward ADSCs supplemented by PRP as well as glycosaminoglycans (GAG) concentration were conducted later. The results showed that higher absorbance of proliferation rate was on ADSCs supplemented by various PRP concentrations compare to FBS control group for seven days of observation. Level of GAG, which secreted by ADSCs supplemented by a various concentration of PRP, was also higher than the FBS group. The highest level of GAG on day 21 was observed in 20% PRP group then 10% and 5% PRP, while a group of GAG level is stable at low levels. This study concludes that PRP has the potential to induce chondrogenesis ADSCs which cultured on silk fibroin scaffold.

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