Effect of fetal bovine serum on the growth and survival of insect cell cultures

1970 ◽  
Vol 48 (3) ◽  
pp. 427-432 ◽  
Author(s):  
S. S. Sohi ◽  
Cheryl Smith
Keyword(s):  
Adverse Effect ◽  
Cell Lines ◽  
Insect Cell ◽  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
Maximum Growth ◽  
Growth And Survival ◽  
Fetal Bovine ◽  
High Concentrations ◽  
Insect Cell Lines

The effect of different concentrations of fetal bovine serum (FBS) on the growth and survival of three insect cell lines—Aedes aegypti, Antheraea eucalypti, and Bombyx mori—was studied in a series of 10 experiments. Cell viability was low and no growth occurred in any of the cell lines when FBS was omitted from the medium. Maximum growth of A. aegypti cells was obtained with 10% FBS. There was no further increase in this growth when FBS was increased to 20 and 30%, and neither did the increased concentrations have any appreciable adverse effect on the growth or survival of these cells. Maximum growth of A. eucalypti and B. mori cells was obtained in 5% FBS; the growth of these cells was significantly less in 20 and 30% FBS. Viability of A. eucalypti cells was quite low in 20 and 30% FBS. There was, however, no adverse effect on the viability of B. mori cells at these high concentrations.

scholarly journals Various factors affect lipopolysaccharide sensitization in cell cultures

BioTechniques ◽  
2020 ◽  
Vol 69 (2) ◽  
pp. 126-132
Author(s):  
Nan Yang ◽  
Don D Sin ◽  
Delbert R Dorscheid
Keyword(s):  
Cell Lines ◽  
Cell Cultures ◽  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
Signal Loss ◽  
The Media ◽  
Fetal Bovine

Commercially available lipopolysaccharide (LPS) is commonly used in research. Although protocols for its use are well established, we experienced a loss of LPS responsiveness in our cell cultures despite no obvious experimental changes. Our cell lines were stimulated with LPS and the media quantified for LPS responsiveness via an IL-8 ELISA. We discovered that the major cause of signal loss was differences in fetal bovine serum (FBS) formulation and concentration. One FBS formulation was notably better at eliciting an IL-8 signal than the second FBS, and 10% FBS in media was better at inducing LPS responsiveness than lower concentrations. We urge researchers to be aware of inherent variations in seemingly commonplace reagents as they may be unexpected sources of inconsistencies.

scholarly journals Evaluation of the major royal jelly proteins as an alternative to fetal bovine serum in culturing human cell lines

2016 ◽  
Vol 17 (6) ◽  
pp. 476-483 ◽  
Author(s):  
Di Chen ◽  
Xiao-xuan Xin ◽  
Hao-cheng Qian ◽  
Zhang-yin Yu ◽  
Li-rong Shen
Keyword(s):  
Cell Lines ◽  
Human Cell ◽  
Bovine Serum ◽  
Royal Jelly ◽  
Fetal Bovine Serum ◽  
Human Cell Lines ◽  
Fetal Bovine

Characterization of osteoblastic properties of 7F2 and UMR-106 cultures after acclimation to reduced levels of fetal bovine serum

2008 ◽  
Vol 86 (7) ◽  
pp. 403-415 ◽  
Author(s):  
S. Ganguly ◽  
L.A. Ashley ◽  
C.M. Pendleton ◽  
R.D. Grey ◽  
G.C. Howard ◽  
...  
Keyword(s):  
Cell Lines ◽  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
Phenol Red ◽  
Ph Indicator ◽  
Fetal Bovine ◽  
Experimental Findings ◽  
Umr 106 ◽  
Culture Protocol

Estrogen plays an important role in skeletal physiology by maintaining a remodeling balance between the activity of osteoblasts and osteoclasts. In an attempt to decipher the mechanism through which estrogen elicits its action on osteoblasts, experimentation necessitated the development of a culturing environment reduced in estrogenic compounds. The selected medium (OPTI-MEM) is enriched to sustain cultures under reduced fetal bovine serum (FBS) conditions and is devoid of the pH indicator phenol red, a suspected estrogenic agent. This protocol reduced the concentration of FBS supplementation to 0% through successive 24 h incubations with diminishing amounts of total FBS (1%, 0.1%, and 0%). The protocol does not appear to alter the viability, cell morphology, or osteoblast-like phenotype of 7F2 and UMR-106 cell lines when compared with control cells grown in various concentrations of FBS. Although the rate of mitotic divisions declined, the 7F2 and UMR-106 cultures continued to express osteoblast-specific markers and exhibited estrogen responsiveness. These experimental findings demonstrate that the culture protocol developed did not alter the osteoblast nature of the cell lines and provides a model system to study estrogen’s antiresorptive role on skeletal turnover.

scholarly journals The use of fetal bovine serum as delivery vehicle to improve the uptake and stability of lycopene in cell culture studies

2007 ◽  
Vol 98 (1) ◽  
pp. 226-232 ◽  
Author(s):  
Chen-Yu Lin ◽  
Chin-Shiu Huang ◽  
Miao-Lin Hu
Keyword(s):  
Cell Lines ◽  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
Butylated Hydroxytoluene ◽  
Delivery Vehicle ◽  
Prostate Cell ◽  
Free System ◽  
Improved Stability ◽  
Fetal Bovine ◽  
Du145 Cells

Tetrahydrofuran (THF) has commonly been used to deliver carotenoids to cells but the use of THF is associated with cytotoxicity and low uptake efficiency of carotenoids. Here, we used fetal bovine serum (FBS) as the delivery vehicle for lycopene in comparison with THF, THF containing 0·0025 % butylated hydroxytoluene (THF/BHT), methyl-β-cyclodextrin (M-β-CD) and micelles in two human prostate cancer cell lines, DU145 and PC-3. Lycopene (10 mm) solubilized in THF/BHT and then diluted in FBS at ratios of 5 and 10 gave the highest lycopene uptake in DU145 cells. Using a dilution factor of 10, we found that lycopene (10 μm) carried in FBS in a cell-free system led to significantly less loss of lycopene than in THF, THF/BHT and M-β-CD within 24 h of incubation. Lycopene solubilized in micelles was more stable than that in FBS within 24 h, but the micelle itself led to marked cytotoxicity to DU145 cells. Lycopene at 10 μm in FBS led to significantly higher uptake of lycopene in both cell lines than that in THF, THF/BHT or M-β-CD within 24 h of incubation. When FBS was replaced with lipoprotein-deficient serum, the uptake of lycopene by DU145 cells was markedly decreased and was not significantly different from that of THF or THF/BHT. These results demonstrate that FBS is superior to THF, THF/BHT, M-β-CD and micelles as a delivery vehicle for lycopene in prostate cell lines and that the lipoprotein of FBS is likely responsible for the improved stability and cellular uptake of lycopene.

scholarly journals Albumin Enhances the Rate at Which Coxsackievirus B3 Strain 28 Converts to A-Particles

2020 ◽  
Vol 94 (6) ◽  
Author(s):  
Steven D. Carson ◽  
Andrew J. Cole
Keyword(s):  
Catalytic Activity ◽  
Bovine Serum ◽  
Culture Medium ◽  
Fetal Bovine Serum ◽  
Coxsackievirus B3 ◽  
Target Cells ◽  
Soluble Receptor ◽  
Fetal Bovine ◽  
Capsid Protein Vp1 ◽  
High Concentrations

ABSTRACT Three strains of coxsackievirus B3 (CVB3) differ by single mutations in capsid protein VP1 or VP3 and also differ in stability at 37°C in tissue culture medium. Among these strains, the CVB3/28 parent strain has been found to be uniquely sensitive to a component in fetal bovine serum (FBS) identified as serum albumin. In cell culture medium, serum increased the rate of CVB3/28 conversion to noninfectious particles at least 2-fold. The effect showed a saturable dose response. Rates of conversion to noninfectious virus with high concentrations of soluble coxsackievirus and adenovirus receptor (sCAR) were similar with and without FBS, but FBS amplified the catalytic effect of 100 nM sCAR nearly 3-fold. Such effects in other systems are due to nonessential activating cofactors. IMPORTANCE A factor other than the virus receptor expressed by target cells has been found to accelerate the loss of an enterovirus (CVB3/28) infectious titer, with little effect on nearly identical mutant strains. The destabilizing factor in fetal bovine serum, identified as albumin, does not interfere with the catalytic activity of soluble receptor at saturating receptor concentrations and amplifies the catalytic activity of the soluble receptor at a concentration that otherwise produces about one-third the saturated receptor-catalyzed rate of virus decay. This finding evidences the possibility that other virus-“priming” ligands may also be nonessential activating cofactors that serve to accelerate receptor-catalyzed viral eclipse.

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