scholarly journals Optimization of staining with SYTO 9/propidium iodide: interplay, kinetics and impact on Brevibacillus brevis

BioTechniques ◽  
2020 ◽  
Vol 69 (2) ◽  
pp. 88-98
Author(s):  
Ying Deng ◽  
Lili Wang ◽  
Yujia Chen ◽  
Yan Long
Keyword(s):  
Flow Cytometry ◽  
Cell Wall ◽  
Physicochemical Properties ◽  
Environmental Monitoring ◽  
Propidium Iodide ◽  
Food Industry ◽  
Actual Performance ◽  
Brevibacillus Brevis ◽  
Syto 9

Fluorophores SYTO 9 and propidium iodide (PI) are extensively applied in medicine, food industry and environmental monitoring to assess the viability of bacteria. However, the actual performance of these dyes remains largely unknown. In addition, their effects on the physiology of cells have not been elucidated. Here we characterized the effects of these two dyes on Brevibacillus brevis under optimized staining. We found that SYTO 9 entered cells continuously while PI tended to adhere to the cell wall before entering the cell. In addition, results showed that a high amount of the dyes altered the physicochemical properties of membranes, improving their breakthrough. These results provide new perspectives and ideas for improving the characterization of bacterial viability using flow cytometry.

Isolation and evaluation of some physicochemical properties of Parkia biglobosa starch

2009 ◽  
Vol 81 (1) ◽  
pp. 97-104 ◽  
Author(s):  
Nnemeka Edith Ihegwuagu ◽  
Moses Olubayo Omojola ◽  
Martins Ochobiojo Emeje ◽  
Olobayo Olufunsho Kunle
Keyword(s):  
Physicochemical Properties ◽  
Food Industry ◽  
Corn Starch ◽  
Physicochemical Characterization ◽  
Parkia Biglobosa ◽  
Locust Bean ◽  
African Locust Bean

This paper describes the isolation and physicochemical characterization of starch obtained from Parkia biglobosa (African locust bean). The results show that the properties of starch from this source compare favorably with those of corn starch BP, and suggest that it could be useful as a stabilizer in baking powders and as an emulsifier in the food industry.

Characterization of Different Types of Biomass Wastes Using Thermogravimetric and ICP-MS Analyses

2020 ◽  
Vol 70 (12) ◽  
pp. 4594-4600
Keyword(s):  
Thermogravimetric Analysis ◽  
Food Industry ◽  
Wood Waste ◽  
Biomass Ash ◽  
Minor Elements ◽  
Ms Analysis ◽  
Icp Ms ◽  
Different Types

The purpose of this study was to characterize some types of biomass wastes resulted from different activities such as: agriculture, forestry and food industry using thermogravimetric and ICP-MS analyses. Also, it was optimized an ICP-MS method for the determination of As, Cd and Pb from biomass ash samples. The ICP-MS analysis revealed that the highest concentration of metals (As, Cd, Pb) was recorded in the wood waste ash sample, also the thermogravimetric analysis indicated that the highest amount of ash was obtained for the same sample (26.82%). The biomass wastes mentioned in this study are alternative recyclable materials, reusable as pellets and briquettes. Keywords: ash, biomass, ICP-MS, minor elements, TG

Synthesis, Characterization, and Dynamic Behavior of Well-defined Dithiomaleimide-functionalized Maltodextrins

2020 ◽  
Vol 17 (2) ◽  
pp. 85-89
Author(s):  
Francisco J. Hidalgo ◽  
Nathan A.P. Lorentz ◽  
TinTin B. Luu ◽  
Jonathan D. Tran ◽  
Praveen D. Wickremasinghe ◽  
...  
Keyword(s):  
Physicochemical Properties ◽  
Dynamic Behavior ◽  
Industrial Applications ◽  
19F Nmr ◽  
Nmr Studies ◽  
Synthetic Pathway ◽  
End Groups ◽  
Sugar End

: Maltodextrins have an increasing number of biomedical and industrial applications due to their attractive physicochemical properties such as biodegradability and biocompatibility. Herein, we describe the development of a synthetic pathway and characterization of thiol-responsive maltodextrin conjugates with dithiomaleimide linkages. 19F NMR studies were also conducted to demonstrate the exchange dynamics of the dithiomaleimide-functionalized sugar end groups.

Characterization of the Lipid Fraction of Cocoplum Seeds (Chrysobalanusicaco, L) for Use in the Food Industry

2017 ◽  
Vol 7 (3) ◽  
Author(s):  
Andrea Almeida Mello ◽  
Cristiane Hess Azevedo-Meleiro ◽  
Armando Ubirajara Oliveira Sabaa-Srur ◽  
Renato Grimaldi ◽  
Robert E. Smith
Keyword(s):  
Food Industry ◽  
Lipid Fraction

Chemical characterization of tequila maturation process and their connection with the physicochemical properties of the cask

2021 ◽  
Vol 98 ◽  
pp. 103804
Author(s):  
Walter M. Warren-Vega ◽  
Rocío Fonseca-Aguiñaga ◽  
Linda V. González-Gutiérrez ◽  
Francisco Carrasco-Marín ◽  
Ana I. Zárate-Guzmán ◽  
...  
Keyword(s):  
Physicochemical Properties ◽  
Chemical Characterization ◽  
Maturation Process

scholarly journals Physicochemical Characterization Cascade of Nanoadjuvant–Antigen Systems for Improving Vaccines

Vaccines ◽  
2021 ◽  
Vol 9 (6) ◽  
pp. 544
Author(s):  
Giuditta Guerrini ◽  
Antonio Vivi ◽  
Sabrina Gioria ◽  
Jessica Ponti ◽  
Davide Magrì ◽  
...  
Keyword(s):  
Immune Response ◽  
Protein Structure ◽  
Physicochemical Properties ◽  
Physicochemical Characterization ◽  
Protein Antigen ◽  
Efficacy And Safety

Adjuvants have been used for decades to enhance the immune response to vaccines, in particular for the subunit-based adjuvants. Physicochemical properties of the adjuvant-protein antigen complexes, such as size, morphology, protein structure and binding, influence the overall efficacy and safety of the vaccine. Here we show how to perform an accurate physicochemical characterization of the nanoaluminum–ovalbumin complex. Using a combination of existing techniques, we developed a multi-staged characterization strategy based on measurements of increased complexity. This characterization cascade has the advantage of being very flexible and easily adaptable to any adjuvant-protein antigen combinations. It will contribute to control the quality of antigen–adjuvant complexes and immunological outcomes, ultimately leading to improved vaccines.

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