Identification and Characterization of Species-Specific SARS-CoV-2 Physicochemical Properties

2020 ◽  
Author(s):  
Srinivasulu Yerukala Sathipati ◽  
Shinn-Ying Ho
Keyword(s):  
Physicochemical Properties ◽  
Species Specific ◽  
Identification And Characterization

scholarly journals Identification and Characterization of Benomyl-Resistant and -Sensitive Populations of Colletotrichum gloeosporioides from Statice (Limonium spp.)

2006 ◽  
Vol 96 (5) ◽  
pp. 542-548 ◽  
Author(s):  
Marcel Maymon ◽  
Aida Zveibil ◽  
Shimon Pivonia ◽  
Dror Minz ◽  
Stanley Freeman
Keyword(s):  
Colletotrichum Gloeosporioides ◽  
Sequence Analyses ◽  
Specific Primers ◽  
Tubulin Genes ◽  
Colletotrichum Spp ◽  
Polymerase Chain ◽  
Species Specific ◽  
Identification And Characterization ◽  
Propagation Material

Sixty-four isolates of Colletotrichum gloeosporioides were isolated from infected Limonium spp. cultivated in 12 different locations in Israel. All isolates were identified as belonging to the C. gloeosporioides complex by species-specific primers. Of these isolates, 46 were resistant to benomyl at 10 μg/ml and 18 were sensitive to this concentration of fungicide. Based on arbitrarily primed polymerase chain reaction of all isolates and internal transcribed spacer-1 sequence analyses of 12 selected isolates, the benomyl-resistant and -sensitive populations belong to two distinct genotypes. Sequence analyses of the β-tubulin genes, TUB1 and TUB2, of five sensitive and five resistant representative isolates of C. gloeosporioides from Limonium spp. revealed that the benomyl-resistant isolates had an alanine substitute instead of a glutamic acid at position 198 in TUB2. All data suggest that the resistant and sensitive genotypes are two independent and separate populations. Because all Limonium plant propagation material is imported from various geographic regions worldwide, and benomyl is not applied to this crop or for the control of Colletotrichum spp. in Israel, it is presumed that plants are bearing quiescent infections from the points of origin prior to arrival.

scholarly journals Identification and characterization of the 2D6 and Mr 23000 antigens on the plasma membrane of rat spermatozoa

1987 ◽  
Vol 241 (2) ◽  
pp. 353-360 ◽  
Author(s):  
R Jones ◽  
C R Brown
Keyword(s):  
Plasma Membrane ◽  
Antigenic Determinant ◽  
Egg Recognition ◽  
Western Blots ◽  
One Dimensional ◽  
Antigen Present ◽  
Membrane Bound ◽  
Species Specific ◽  
Identification And Characterization

Previous investigations [Jones, Brown, von Glos & Gaunt (1985) Exp. Cell Res. 156, 31-44] have demonstrated the appearance of a new antigenic determinant (recognized by monoclonal antibody 2D6) on the plasma membrane of rat spermatozoa during post-testicular maturation in the epididymis. Identification of the 2D6 antigen on Western blots from one-dimensional SDS/polyacrylamide gels revealed that it co-migrated with a membrane protein (designated Mr 23,000 antigen) present on testicular and immature germ cells, suggesting that one antigen might be a modified version of the other. In the present work, however, we demonstrate that, although they have similar Mr and are present in soluble and membrane-bound forms, the 2D6 and Mr 23,000 antigens are biochemically and immunologically distinct molecules. The properties of the antigens are described and compared. The Mr 23,000 antigen is present on both testicular and cauda epididymidal spermatozoa, has a pI of 6.1, contains no detectable carbohydrate, is not tissue-specific and is degraded by V8 protease. By contrast, the 2D6 antigen is glycosylated, has a broad pI from 4.5 to 6.1, is tissue- and species-specific and is resistant to digestion with V8 protease. Its role in sperm-egg recognition is discussed.

Molecular Identification and Characterization of Chicken Parvovirus from Indian Chicken and Association with Runting and Stunting Syndrome

2020 ◽  
Author(s):  
M. Pradeep ◽  
M. R. Reddy ◽  
T. R. Kannaki
Keyword(s):  
Sequence Analysis ◽  
Close Association ◽  
Enteric Viruses ◽  
Chain Reaction ◽  
Specific Primers ◽  
Avian Nephritis Virus ◽  
Etiological Agents ◽  
Species Specific ◽  
Identification And Characterization

Background: Runting-stunting syndrome (RSS) in chickens is a worldwide problem, attributed with several etiological agents. The present study aimed to identify the association of enteric viruses with RSS in different chicken flocks. Methods: Intestinal samples from 14 flocks of chicken of different age and breed and with or without RSS were collected randomly from necropsy samples, isolated nucleic acids, and screened for major enteric viruses by Polymerised chain reaction (PCR), using species-specific primers. Result: Chicken Parvovirus (ChPV) was identified in 100% of the flocks with RSS, in two of which ChPV alone was detected. While in others it was associated with Avian nephritis virus, Avian Rotavirus, Chicken astrovirus, and Fowl adenovirus in 80%, 50%, 30% and 10% flocks, respectively. RSS was reproduced and isolated ChPV by chicken embryo inoculation using the samples from ChPV alone infected cases. Sequence analysis of ChPV revealed closer association with Ecuodor isolates than the Asian isolates. The results indicated the presence of ChPV in Indian chicken flocks and its close association with RSS.

scholarly journals Study of enterococci during cheese manufacture and ripening and evaluation of their role in tyramine production

2009 ◽  
Vol 57 (5) ◽  
pp. 49-56 ◽  
Author(s):  
Radka Burdychová
Keyword(s):  
Biogenic Amine ◽  
Tyrosine Decarboxylase ◽  
Specific Primers ◽  
Specific Pcr ◽  
Gene Coding ◽  
Species Specific ◽  
The Relationship ◽  
Identification And Characterization ◽  
Hard Cheese

The aim of this study was isolation, identification and characterization of bacteria of the genusEnterococcusfrom Duch-type semi-hard cheese during manufacture and ripening. Cheese samples from two different producers (I and II) were used at the production day and after 30, 90 and 176 days of ripening.Altogether 361 suspected enterococci isolates were obtained from cheese samples during 7 month of ripening. Using genus-specific PCR, 285 isolates were identified as the members of the genusEnterococcus. The identification of fiveEnterococcusspecies was performed by PCR using species-specific primers. Among 165Enterococcusspp. isolates of producer I, 81 isolates were classified asE. faecium, 39 asE. durans, 21 asE. faecalis, 19 asE. casseliflavusand 3 asE. hirae, and 2 isolates were not classified into species. Enterococci species among isolates of producer II were as follows: 52 isolates ofE. faecium, 38 ofE. faecalis, 14 ofE. durans, 12 ofE. casseliflavus, 3 ofE. hiraeand 1 was not classified into species.E. faeciumwas found to be the dominating species in all cheese samples. The gene coding for tyrosine decarboxylase was detected in 10 enterococci isolates of producer I and in 5 enterococci isolates of producer II. Production of biogenic amine tyramine was confirmed in all these isolates, which were ofE. faecium,E. faecalisandE. duransspecies. It was confirmed that these species are important for tyramine production. There is the relationship between tyramine production and counts ofE. faecium,E. faecalisandE. durans. No tyramine production was observed in isolates ofE. casseliflavusorE. hiraespecies.

scholarly journals Differentiation of Borrelia burgdorferiSensu Lato on the Basis of RNA Polymerase Gene (rpoB) Sequences

2000 ◽  
Vol 38 (7) ◽  
pp. 2557-2562 ◽  
Author(s):  
Seung-Hyun Lee ◽  
Bum-Joon Kim ◽  
Jong-Hyun Kim ◽  
Kyung-Hee Park ◽  
Seo-Jeong Kim ◽  
...  
Keyword(s):  
Amino Acid ◽  
Restriction Analysis ◽  
Direct Sequencing ◽  
Amino Acid Sequences ◽  
Amino Acid Residues ◽  
Borrelia Lusitaniae ◽  
Primer Sets ◽  
Species Specific ◽  
Identification And Characterization

We determined the nucleotide sequences (329 bp) of therpoB DNAs from 22 reference strains ofBorrelia. No insertions or deletions were observed. Deduced amino acid sequences of amplified rpoB DNA comprised 109 amino acid residues (N450 to M558[Escherichia coli numbering]). All amino acid sequences were identical with the exception of those of Borrelia lusitaniae PotiB2 (T461→A) and B. bissettii DN127 (I498→V). Each species of B. burgdorferi sensu lato was differentiated as a distinct entity in the phylogenetic tree constructed by a neighbor-joining method. B. burgdorferi sensu lato could be distinguished from B. turicatae and B. hermsii, which are associated with relapsing fever. Seventeen Korean isolates could be identified by PCR-linked direct sequencing and restriction analysis of the rpoB DNA. These results suggest that rpoB DNA is useful for identification and characterization of Borrelia. In addition, we developed the rapid species identification method using the species-specific primer sets based on rpoB gene sequences.

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