scholarly journals Novel Signal Amplification Technology with Applications in DNA and Protein Detection Systems

BioTechniques ◽  
2001 ◽  
Vol 30 (6) ◽  
pp. 1268-1272
Author(s):  
C.M. Lisle ◽  
S. Bortolin ◽  
A.S. Benight ◽  
R.A. Janeczko ◽  
R.L. Zastawny
Keyword(s):  
Signal Amplification ◽  
Protein Detection ◽  
Detection Systems

scholarly journals Protein detection enhanced by 3DNA dendrimer signal amplification

BioTechniques ◽  
2008 ◽  
Vol 44 (6) ◽  
pp. 815-818 ◽  
Author(s):  
Johanna R. Mora ◽  
Tamara L. Zielinski ◽  
Bryce P. Nelson ◽  
Robert C. Getts
Keyword(s):  
Signal Amplification ◽  
Protein Detection

slowly growing natural populations. Various approaches have been adopted in order to improve the sensitivity. These have included the use of multiple probes labelled with a single fluor (Lee et al. 1993); or labelled with multiple fluors (Trebesius et al. 1994) and enzyme-linked probes or detection systems that allow signal amplification (Lebaron et al. 1997, Schonhuber et al. 1999). The latter indirect approach not only has the potential for signal amplification, but may also be used in natural samples showing high levels of autofluorescence. Any thorough identification method has to include positive and negative controls. False-positive results may either be caused by cells emitting autofluorescence upon excitation or by nonspecific binding of the probe to nontarget cells. Samples should therefore be checked for autofluorescence before hybridization and a negative control with a fluorescent oligonucleotide not complementary to rRNA has to be applied to check for sequence-independent nonspecific binding. Such non-specific binding may be due to interaction of the dye compound of the probe with hydrophobic cell components. Failures to detect cells containing target sequences (false-negatives) may originate from cells with either low cellular ribosome content or limited permeability of the cell periphery for the fluorescent probe (Manz et al. 1992). With the rapidly expanding database of 16S rRNA sequences, the problem of probe specificity has become more apparent and the design of probes is becoming increasingly difficult. These problems are also applicable to PCR and other oligonucleotide-dependent techniques. The problem of probe specificity may be overcome by using multiple specific oligonucleotide probes targeting different sites on the rRNA molecule and labelled with different fluorochromes. While a single oligonucleotide target sequence may be found in a number of related taxa, the probability that target sites for three designed oligonucleotides are found in a nontarget organism is, however, much reduced.

2003 ◽  
pp. 232-232
Keyword(s):  
Signal Amplification ◽  
Specific Binding ◽  
Natural Populations ◽  
Negative Control ◽  
Target Sequence ◽  
Cell Periphery ◽  
Nonspecific Binding ◽  
Detection Systems ◽  
Cell Components ◽  
Negative Controls

scholarly journals Highly sensitive and multiplexed in situ protein profiling with cleavable fluorescent streptavidin

2019 ◽  
Author(s):  
Renjie Liao ◽  
Diego Mastroeni ◽  
Paul D. Coleman ◽  
Jia Guo
Keyword(s):  
Signal Amplification ◽  
Individual Cell ◽  
Protein Detection ◽  
Protein Profiling ◽  
Detection Sensitivity ◽  
Layer By Layer ◽  
Protein Targets ◽  
Highly Sensitive ◽  
Wide Range

AbstractThe ability to perform highly sensitive and multiplexed in situ protein analysis is crucial to advance our understanding of normal physiology and disease pathogenesis. To achieve this goal, here we develop an approach using cleavable biotin conjugated antibodies and cleavable fluorescent streptavidin (CFS). In this approach, protein targets are first recognized by the cleavable biotin labeled antibodies. Subsequently, CFS is applied to stain the protein targets. Though layer-by-layer signal amplification using cleavable biotin conjugated orthogonal antibodies and CSF, the protein detection sensitivity can be enhanced by at least 10 fold, compared with the existing methods. After imaging, the fluorophores and the biotins unbound to streptavidin are removed by chemical cleavage. The leftover streptavidin is blocked by biotin. Upon reiterative analysis cycles, a large number of different proteins with a wide range of expression levels can be unambiguously detected in individual cell in situ.

Detection of thrombin based on aptamer isothermal exponential signal amplification technique

The Analyst ◽  
2015 ◽  
Vol 140 (19) ◽  
pp. 6489-6492 ◽  
Author(s):  
Xiaotong Shen ◽  
Menghua Zhang ◽  
Shuyan Niu ◽  
Chao Shi
Keyword(s):  
Signal Amplification ◽  
Protein Detection ◽  
Amplification Technique

In this report, a strategy based on an aptameric molecule switch to realize homogeneous and isothermal signal exponential amplification and protein detection has been described.

Proximity-dependent protein detection based on enzyme-assisted fluorescence signal amplification

2014 ◽  
Vol 51 ◽  
pp. 255-260 ◽  
Author(s):  
Yuyu Tan ◽  
Qiuping Guo ◽  
Xiayu Zhao ◽  
Xiaohai Yang ◽  
Kemin Wang ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Protein Detection ◽  
Fluorescence Signal ◽  
Fluorescence Signal Amplification ◽  
Dependent Protein

scholarly journals Lateral Flow Immunoassay Coupled with Copper Enhancement for Rapid and Sensitive SARS-CoV-2 Nucleocapsid Protein Detection

Biosensors ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 13
Author(s):  
Tao Peng ◽  
Xueshima Jiao ◽  
Zhanwei Liang ◽  
Hongwei Zhao ◽  
Yang Zhao ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Nucleocapsid Protein ◽  
High Efficiency ◽  
Protein Detection ◽  
Antigen Detection ◽  
Lateral Flow ◽  
Copper Deposition ◽  
Detection Sensitivity ◽  
Rapid Screening ◽  
Lateral Flow Immunoassay

The coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory coronavirus 2 (SARS-CoV-2) is still raging all over the world. Hence, the rapid and sensitive screening of the suspected population is in high demand. The nucleocapsid protein (NP) of SARS-CoV-2 has been selected as an ideal marker for viral antigen detection. This study describes a lateral flow immunoassay (LFIA) based on colloidal gold nanoparticles for rapid NP antigen detection, in which sensitivity was improved through copper deposition-induced signal amplification. The detection sensitivity of the developed LFIA for NP antigen detection (using certified reference materials) under the optimized parameters was 0.01 μg/mL and was promoted by three orders of magnitude to 10 pg/mL after copper deposition signal amplification. The LFIA coupled with the copper enhancement technique has many merits such as low cost, high efficiency, and high sensitivity. It provides an effective approach to the rapid screening, diagnosis, and monitoring of the suspected population in the COVID-19 outbreak.

Electrochemical detection of protein by using magnetic graphene-based target enrichment and copper nanoparticles-assisted signal amplification

The Analyst ◽  
2015 ◽  
Vol 140 (22) ◽  
pp. 7818-7822 ◽  
Author(s):  
Jing Zhao ◽  
Yun Lv ◽  
Mingyang Kang ◽  
Keming Wang ◽  
Yang Xiang
Keyword(s):  
Electrochemical Detection ◽  
Copper Nanoparticles ◽  
Signal Amplification ◽  
Electrochemical Method ◽  
Protein Detection ◽  
Duplex Dna ◽  
Target Enrichment ◽  
Magnetic Graphene

A new electrochemical method for protein detection has been proposed based on magnetic graphene and duplex DNA-templated copper nanoparticles.

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