Detection of thrombin based on aptamer isothermal exponential signal amplification technique

The Analyst ◽  
2015 ◽  
Vol 140 (19) ◽  
pp. 6489-6492 ◽  
Author(s):  
Xiaotong Shen ◽  
Menghua Zhang ◽  
Shuyan Niu ◽  
Chao Shi
Keyword(s):  
Signal Amplification ◽  
Protein Detection ◽  
Amplification Technique

In this report, a strategy based on an aptameric molecule switch to realize homogeneous and isothermal signal exponential amplification and protein detection has been described.

scholarly journals Protein detection enhanced by 3DNA dendrimer signal amplification

BioTechniques ◽  
2008 ◽  
Vol 44 (6) ◽  
pp. 815-818 ◽  
Author(s):  
Johanna R. Mora ◽  
Tamara L. Zielinski ◽  
Bryce P. Nelson ◽  
Robert C. Getts
Keyword(s):  
Signal Amplification ◽  
Protein Detection

scholarly journals Novel Signal Amplification Technology with Applications in DNA and Protein Detection Systems

BioTechniques ◽  
2001 ◽  
Vol 30 (6) ◽  
pp. 1268-1272
Author(s):  
C.M. Lisle ◽  
S. Bortolin ◽  
A.S. Benight ◽  
R.A. Janeczko ◽  
R.L. Zastawny
Keyword(s):  
Signal Amplification ◽  
Protein Detection ◽  
Detection Systems

scholarly journals Highly sensitive and multiplexed in situ protein profiling with cleavable fluorescent streptavidin

2019 ◽  
Author(s):  
Renjie Liao ◽  
Diego Mastroeni ◽  
Paul D. Coleman ◽  
Jia Guo
Keyword(s):  
Signal Amplification ◽  
Individual Cell ◽  
Protein Detection ◽  
Protein Profiling ◽  
Detection Sensitivity ◽  
Layer By Layer ◽  
Protein Targets ◽  
Highly Sensitive ◽  
Wide Range

AbstractThe ability to perform highly sensitive and multiplexed in situ protein analysis is crucial to advance our understanding of normal physiology and disease pathogenesis. To achieve this goal, here we develop an approach using cleavable biotin conjugated antibodies and cleavable fluorescent streptavidin (CFS). In this approach, protein targets are first recognized by the cleavable biotin labeled antibodies. Subsequently, CFS is applied to stain the protein targets. Though layer-by-layer signal amplification using cleavable biotin conjugated orthogonal antibodies and CSF, the protein detection sensitivity can be enhanced by at least 10 fold, compared with the existing methods. After imaging, the fluorophores and the biotins unbound to streptavidin are removed by chemical cleavage. The leftover streptavidin is blocked by biotin. Upon reiterative analysis cycles, a large number of different proteins with a wide range of expression levels can be unambiguously detected in individual cell in situ.

Sensitive detection of microRNA using QCM biosensors: sandwich hybridization and signal amplification by TiO2 nanoparticles

2020 ◽  
Vol 12 (42) ◽  
pp. 5103-5109
Author(s):  
Ji Yoon Lim ◽  
Soo Suk Lee
Keyword(s):  
Tio2 Nanoparticles ◽  
Signal Amplification ◽  
Sensitive Detection ◽  
Sandwich Hybridization ◽  
Amplification Technique

A QCM biosensor for the detection of miR-21 has been demonstrated along with sandwich hybridization and TiO2 nanoparticle-based photocatalytic signal amplification technique.

Proximity-dependent protein detection based on enzyme-assisted fluorescence signal amplification

2014 ◽  
Vol 51 ◽  
pp. 255-260 ◽  
Author(s):  
Yuyu Tan ◽  
Qiuping Guo ◽  
Xiayu Zhao ◽  
Xiaohai Yang ◽  
Kemin Wang ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Protein Detection ◽  
Fluorescence Signal ◽  
Fluorescence Signal Amplification ◽  
Dependent Protein

Enzyme-assisted target recycling (EATR) for nucleic acid detection

2014 ◽  
Vol 43 (17) ◽  
pp. 6405-6438 ◽  
Author(s):  
Yulia V. Gerasimova ◽  
Dmitry M. Kolpashchikov
Keyword(s):  
Nucleic Acid ◽  
Signal Amplification ◽  
Nucleic Acid Detection ◽  
Target Recycling ◽  
Amplification Technique

Enzyme-assisted target recycling (EATR) is a signal amplification technique that can find application in PCR-free nucleic acid detection.

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