Electrochemical detection of protein by using magnetic graphene-based target enrichment and copper nanoparticles-assisted signal amplification

The Analyst ◽  
2015 ◽  
Vol 140 (22) ◽  
pp. 7818-7822 ◽  
Author(s):  
Jing Zhao ◽  
Yun Lv ◽  
Mingyang Kang ◽  
Keming Wang ◽  
Yang Xiang
Keyword(s):  
Electrochemical Detection ◽  
Copper Nanoparticles ◽  
Signal Amplification ◽  
Electrochemical Method ◽  
Protein Detection ◽  
Duplex Dna ◽  
Target Enrichment ◽  
Magnetic Graphene

A new electrochemical method for protein detection has been proposed based on magnetic graphene and duplex DNA-templated copper nanoparticles.

scholarly journals Protein detection enhanced by 3DNA dendrimer signal amplification

BioTechniques ◽  
2008 ◽  
Vol 44 (6) ◽  
pp. 815-818 ◽  
Author(s):  
Johanna R. Mora ◽  
Tamara L. Zielinski ◽  
Bryce P. Nelson ◽  
Robert C. Getts
Keyword(s):  
Signal Amplification ◽  
Protein Detection

scholarly journals Novel Signal Amplification Technology with Applications in DNA and Protein Detection Systems

BioTechniques ◽  
2001 ◽  
Vol 30 (6) ◽  
pp. 1268-1272
Author(s):  
C.M. Lisle ◽  
S. Bortolin ◽  
A.S. Benight ◽  
R.A. Janeczko ◽  
R.L. Zastawny
Keyword(s):  
Signal Amplification ◽  
Protein Detection ◽  
Detection Systems

scholarly journals Highly sensitive and multiplexed in situ protein profiling with cleavable fluorescent streptavidin

2019 ◽  
Author(s):  
Renjie Liao ◽  
Diego Mastroeni ◽  
Paul D. Coleman ◽  
Jia Guo
Keyword(s):  
Signal Amplification ◽  
Individual Cell ◽  
Protein Detection ◽  
Protein Profiling ◽  
Detection Sensitivity ◽  
Layer By Layer ◽  
Protein Targets ◽  
Highly Sensitive ◽  
Wide Range

AbstractThe ability to perform highly sensitive and multiplexed in situ protein analysis is crucial to advance our understanding of normal physiology and disease pathogenesis. To achieve this goal, here we develop an approach using cleavable biotin conjugated antibodies and cleavable fluorescent streptavidin (CFS). In this approach, protein targets are first recognized by the cleavable biotin labeled antibodies. Subsequently, CFS is applied to stain the protein targets. Though layer-by-layer signal amplification using cleavable biotin conjugated orthogonal antibodies and CSF, the protein detection sensitivity can be enhanced by at least 10 fold, compared with the existing methods. After imaging, the fluorophores and the biotins unbound to streptavidin are removed by chemical cleavage. The leftover streptavidin is blocked by biotin. Upon reiterative analysis cycles, a large number of different proteins with a wide range of expression levels can be unambiguously detected in individual cell in situ.

Ultrasensitive electrochemical detection of Hg2+based on an Hg2+-triggered exonuclease III-assisted target recycling strategy

The Analyst ◽  
2018 ◽  
Vol 143 (23) ◽  
pp. 5771-5778 ◽  
Author(s):  
Xiaolei Song ◽  
Yu Wang ◽  
Su Liu ◽  
Xue Zhang ◽  
Haiwang Wang ◽  
...  
Keyword(s):  
Electrochemical Detection ◽  
Electrochemical Method ◽  
Sensitive Detection ◽  
Mercury Ions ◽  
Exonuclease Iii ◽  
Target Recycling ◽  
Highly Sensitive ◽  
Recycling Amplification ◽  
Highly Sensitive Detection

An isothermal electrochemical method for the highly sensitive detection of mercury ions (Hg2+) was established based on Hg2+-triggered exonuclease III-aided target recycling amplification.

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