Presence and removal of a contaminating NADH oxidation activity in recombinant maltose-binding protein fusion proteins expressed in Escherichia coli

Fengguang Guo; Guan Zhu

doi:10.2144/0000113822

Presence and removal of a contaminating NADH oxidation activity in recombinant maltose-binding protein fusion proteins expressed in Escherichia coli

BioTechniques ◽

10.2144/0000113822 ◽

2012 ◽

Vol 52 (4) ◽

Cited By ~ 10

Author(s):

Fengguang Guo ◽

Guan Zhu

Keyword(s):

Escherichia Coli ◽

Fusion Proteins ◽

Binding Protein ◽

Maltose Binding Protein ◽

Nadh Oxidation ◽

Protein Fusion ◽

Oxidation Activity ◽

Maltose Binding Protein Fusion

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Strategy for purifying maltose binding protein fusion proteins by affinity precipitation

Journal of Chromatography A ◽

10.1016/j.chroma.2008.04.029 ◽

2008 ◽

Vol 1194 (1) ◽

pp. 90-95 ◽

Cited By ~ 10

Author(s):

Smita Raghava ◽

Samina Aquil ◽

Sanchari Bhattacharyya ◽

Raghavan Varadarajan ◽

Munishwar N. Gupta

Keyword(s):

Fusion Proteins ◽

Binding Protein ◽

Maltose Binding Protein ◽

Protein Fusion ◽

Affinity Precipitation ◽

Maltose Binding Protein Fusion

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A generic protocol for the expression and purification of recombinant proteins in Escherichia coli using a combinatorial His6-maltose binding protein fusion tag

Nature Protocols ◽

10.1038/nprot.2007.50 ◽

2007 ◽

Vol 2 (2) ◽

pp. 383-391 ◽

Cited By ~ 56

Author(s):

Sreedevi Nallamsetty ◽

David S Waugh

Keyword(s):

Escherichia Coli ◽

Recombinant Proteins ◽

Binding Protein ◽

Maltose Binding Protein ◽

Protein Fusion ◽

Expression And Purification ◽

Fusion Tag ◽

Maltose Binding Protein Fusion

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Engineering of a proteolytically stable human β2-adrenergic receptor/maltose-binding protein fusion and production of the chimeric protein in Escherichia coli and baculovirus-infected insect cells

Journal of Biotechnology ◽

10.1016/s0168-1656(99)00216-3 ◽

2000 ◽

Vol 77 (2-3) ◽

pp. 219-234 ◽

Cited By ~ 26

Author(s):

W Hampe

Keyword(s):

Escherichia Coli ◽

Adrenergic Receptor ◽

Insect Cells ◽

Binding Protein ◽

Chimeric Protein ◽

Maltose Binding Protein ◽

Protein Fusion ◽

Maltose Binding Protein Fusion ◽

Infected Insect ◽

Β2 Adrenergic Receptor

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Interaction of the muscarinic acetylcholine receptor M2 subtype with G protein G i/o isotypes and G subunits as studied with the maltose-binding protein-M2-G i/o fusion proteins expressed in Escherichia coli

The Journal of Biochemistry ◽

10.1093/jb/mvu036 ◽

2014 ◽

Vol 156 (5) ◽

pp. 259-272

Author(s):

S. Ichiyama ◽

R. Nemoto ◽

H. Tanabe ◽

T. Haga

Keyword(s):

Escherichia Coli ◽

G Protein ◽

Acetylcholine Receptor ◽

Fusion Proteins ◽

Binding Protein ◽

Muscarinic Acetylcholine Receptor ◽

Maltose Binding Protein ◽

Protein G ◽

Muscarinic Acetylcholine

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Single amino acid substitutions on the surface of Escherichia coli maltose-binding protein can have a profound impact on the solubility of fusion proteins

Protein Science ◽

10.1110/ps.45201 ◽

2001 ◽

Vol 10 (3) ◽

pp. 622-630 ◽

Cited By ~ 88

Author(s):

J. D. Fox

Keyword(s):

Escherichia Coli ◽

Amino Acid ◽

Fusion Proteins ◽

Binding Protein ◽

Maltose Binding Protein ◽

Single Amino Acid ◽

Amino Acid Substitutions ◽

Profound Impact

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Structure of an Engineered β-Lactamase Maltose Binding Protein Fusion Protein: Insights into Heterotropic Allosteric Regulation

PLoS ONE ◽

10.1371/journal.pone.0039168 ◽

2012 ◽

Vol 7 (6) ◽

pp. e39168 ◽

Cited By ~ 12

Author(s):

Wei Ke ◽

Abigail H. Laurent ◽

Morgan D. Armstrong ◽

Yuchao Chen ◽

William E. Smith ◽

...

Keyword(s):

Fusion Protein ◽

Binding Protein ◽

Allosteric Regulation ◽

Maltose Binding Protein ◽

Protein Fusion ◽

Maltose Binding Protein Fusion

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A peptide/maltose-binding protein fusion protein used to replace the traditional antigen for immunological detection of deoxynivalenol in food and feed

Food Chemistry ◽

10.1016/j.foodchem.2018.06.096 ◽

2018 ◽

Vol 268 ◽

pp. 242-248 ◽

Cited By ~ 10

Author(s):

Yang Xu ◽

Hongwei Yang ◽

Zhibing Huang ◽

Yanping Li ◽

Qinghua He ◽

...

Keyword(s):

Fusion Protein ◽

Binding Protein ◽

Maltose Binding Protein ◽

Protein Fusion ◽

Maltose Binding Protein Fusion ◽

Immunological Detection ◽

Food And Feed

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Cloning, expression, purification and preliminary crystallographic analysis of the RNase HI domain of theMycobacterium tuberculosisprotein Rv2228c as a maltose-binding protein fusion

Acta Crystallographica Section F Structural Biology and Crystallization Communications ◽

10.1107/s1744309108021118 ◽

2008 ◽

Vol 64 (8) ◽

pp. 746-749 ◽

Cited By ~ 2

Author(s):

Harriet A. Watkins ◽

Edward N. Baker

Keyword(s):

Binding Protein ◽

Maltose Binding Protein ◽

Crystallographic Analysis ◽

Protein Fusion ◽

Maltose Binding Protein Fusion ◽

Rnase Hi

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Expression of a single-chain antibody as a maltose binding protein fusion

Protein Engineering Design and Selection ◽

10.1093/protein/6.supplement.88-b ◽

1993 ◽

Keyword(s):

Binding Protein ◽

Maltose Binding Protein ◽

Protein Fusion ◽

Single Chain ◽

Single Chain Antibody ◽

Maltose Binding Protein Fusion

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Maltose-Binding Protein Fusion Allows for High Level Bacterial Expression and Purification of Bioactive Mammalian Cytokine Derivatives

PLoS ONE ◽

10.1371/journal.pone.0106724 ◽

2014 ◽

Vol 9 (9) ◽

pp. e106724 ◽

Cited By ~ 6

Author(s):

Andrea Pennati ◽

Jiusheng Deng ◽

Jacques Galipeau

Keyword(s):

Binding Protein ◽

Maltose Binding Protein ◽

Bacterial Expression ◽

Protein Fusion ◽

Expression And Purification ◽

Maltose Binding Protein Fusion ◽

High Level

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