A generic protocol for the expression and purification of recombinant proteins in Escherichia coli using a combinatorial His6-maltose binding protein fusion tag

Sreedevi Nallamsetty; David S Waugh

doi:10.1038/nprot.2007.50

A generic protocol for the expression and purification of recombinant proteins in Escherichia coli using a combinatorial His6-maltose binding protein fusion tag

Nature Protocols ◽

10.1038/nprot.2007.50 ◽

2007 ◽

Vol 2 (2) ◽

pp. 383-391 ◽

Cited By ~ 56

Author(s):

Sreedevi Nallamsetty ◽

David S Waugh

Keyword(s):

Escherichia Coli ◽

Recombinant Proteins ◽

Binding Protein ◽

Maltose Binding Protein ◽

Protein Fusion ◽

Expression And Purification ◽

Fusion Tag ◽

Maltose Binding Protein Fusion

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High-level expression of soluble recombinant proteins in Escherichia coli using an HE-maltotriose-binding protein fusion tag

Protein Expression and Purification ◽

10.1016/j.pep.2017.09.013 ◽

2018 ◽

Vol 142 ◽

pp. 25-31 ◽

Cited By ~ 10

Author(s):

Yingqian Han ◽

Wanying Guo ◽

Bingqian Su ◽

Yujie Guo ◽

Jiang Wang ◽

...

Keyword(s):

Escherichia Coli ◽

Recombinant Proteins ◽

Binding Protein ◽

Protein Fusion ◽

High Level Expression ◽

Fusion Tag ◽

High Level

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Presence and removal of a contaminating NADH oxidation activity in recombinant maltose-binding protein fusion proteins expressed in Escherichia coli

BioTechniques ◽

10.2144/0000113822 ◽

2012 ◽

Vol 52 (4) ◽

Cited By ~ 10

Author(s):

Fengguang Guo ◽

Guan Zhu

Keyword(s):

Escherichia Coli ◽

Fusion Proteins ◽

Binding Protein ◽

Maltose Binding Protein ◽

Nadh Oxidation ◽

Protein Fusion ◽

Oxidation Activity ◽

Maltose Binding Protein Fusion

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Maltose-Binding Protein Fusion Allows for High Level Bacterial Expression and Purification of Bioactive Mammalian Cytokine Derivatives

PLoS ONE ◽

10.1371/journal.pone.0106724 ◽

2014 ◽

Vol 9 (9) ◽

pp. e106724 ◽

Cited By ~ 6

Author(s):

Andrea Pennati ◽

Jiusheng Deng ◽

Jacques Galipeau

Keyword(s):

Binding Protein ◽

Maltose Binding Protein ◽

Bacterial Expression ◽

Protein Fusion ◽

Expression And Purification ◽

Maltose Binding Protein Fusion ◽

High Level

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Expression, Purification and Characterization of CAR/NCOA-1 Tethered Protein in E. coli Using Maltose-Binding Protein Fusion Tag and Gelatinized Corn Starch

Biological and Pharmaceutical Bulletin ◽

10.1248/bpb.b20-00759 ◽

2021 ◽

Vol 44 (1) ◽

pp. 125-130

Author(s):

Yoshihiro Kobashigawa ◽

Mana Namikawa ◽

Mitsuhiro Sekiguchi ◽

Yuki Inada ◽

Soichiro Yamauchi ◽

...

Keyword(s):

Corn Starch ◽

Binding Protein ◽

Maltose Binding Protein ◽

Protein Fusion ◽

Purification And Characterization ◽

Fusion Tag ◽

E Coli ◽

Maltose Binding Protein Fusion

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Engineering of a proteolytically stable human β2-adrenergic receptor/maltose-binding protein fusion and production of the chimeric protein in Escherichia coli and baculovirus-infected insect cells

Journal of Biotechnology ◽

10.1016/s0168-1656(99)00216-3 ◽

2000 ◽

Vol 77 (2-3) ◽

pp. 219-234 ◽

Cited By ~ 26

Author(s):

W Hampe

Keyword(s):

Escherichia Coli ◽

Adrenergic Receptor ◽

Insect Cells ◽

Binding Protein ◽

Chimeric Protein ◽

Maltose Binding Protein ◽

Protein Fusion ◽

Maltose Binding Protein Fusion ◽

Infected Insect ◽

Β2 Adrenergic Receptor

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Extracellular production and affinity purification of recombinant proteins with Escherichia coli using the versatility of the maltose binding protein

Journal of Biotechnology ◽

10.1016/j.jbiotec.2009.01.010 ◽

2009 ◽

Vol 140 (3-4) ◽

pp. 194-202 ◽

Cited By ~ 12

Author(s):

Benjamin Sommer ◽

Karl Friehs ◽

Erwin Flaschel ◽

Michael Reck ◽

Frank Stahl ◽

...

Keyword(s):

Escherichia Coli ◽

Recombinant Proteins ◽

Binding Protein ◽

Affinity Purification ◽

Maltose Binding Protein ◽

Extracellular Production

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A Generic Method for the Production of Recombinant Proteins in Escherichia coli Using a Dual Hexahistidine-Maltose-Binding Protein Affinity Tag

Methods in Molecular Biology - Macromolecular Crystallography Protocols ◽

10.1007/978-1-59745-209-0_1 ◽

2007 ◽

pp. 1-19 ◽

Cited By ~ 48

Author(s):

Joseph E. Tropea ◽

Scott Cherry ◽

Sreedevi Nallamsetty ◽

Christophe Bignon ◽

David S. Waugh

Keyword(s):

Escherichia Coli ◽

Recombinant Proteins ◽

Binding Protein ◽

Maltose Binding Protein ◽

Affinity Tag ◽

Protein Affinity

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Structure of an Engineered β-Lactamase Maltose Binding Protein Fusion Protein: Insights into Heterotropic Allosteric Regulation

PLoS ONE ◽

10.1371/journal.pone.0039168 ◽

2012 ◽

Vol 7 (6) ◽

pp. e39168 ◽

Cited By ~ 12

Author(s):

Wei Ke ◽

Abigail H. Laurent ◽

Morgan D. Armstrong ◽

Yuchao Chen ◽

William E. Smith ◽

...

Keyword(s):

Fusion Protein ◽

Binding Protein ◽

Allosteric Regulation ◽

Maltose Binding Protein ◽

Protein Fusion ◽

Maltose Binding Protein Fusion

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A peptide/maltose-binding protein fusion protein used to replace the traditional antigen for immunological detection of deoxynivalenol in food and feed

Food Chemistry ◽

10.1016/j.foodchem.2018.06.096 ◽

2018 ◽

Vol 268 ◽

pp. 242-248 ◽

Cited By ~ 10

Author(s):

Yang Xu ◽

Hongwei Yang ◽

Zhibing Huang ◽

Yanping Li ◽

Qinghua He ◽

...

Keyword(s):

Fusion Protein ◽

Binding Protein ◽

Maltose Binding Protein ◽

Protein Fusion ◽

Maltose Binding Protein Fusion ◽

Immunological Detection ◽

Food And Feed

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Cloning, expression, purification and preliminary crystallographic analysis of the RNase HI domain of theMycobacterium tuberculosisprotein Rv2228c as a maltose-binding protein fusion

Acta Crystallographica Section F Structural Biology and Crystallization Communications ◽

10.1107/s1744309108021118 ◽

2008 ◽

Vol 64 (8) ◽

pp. 746-749 ◽

Cited By ~ 2

Author(s):

Harriet A. Watkins ◽

Edward N. Baker

Keyword(s):

Binding Protein ◽

Maltose Binding Protein ◽

Crystallographic Analysis ◽

Protein Fusion ◽

Maltose Binding Protein Fusion ◽

Rnase Hi

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