scholarly journals 2P-104 In vitro analyses of bHLH transcription factors expressed in retinal progenitor cells(The 46th Annual Meeting of the Biophysical Society of Japan)

2008 ◽  
Vol 48 (supplement) ◽  
pp. S91
Author(s):  
Osamu Hisatomi ◽  
Koji Hasegawa ◽  
Daisuke Kitano ◽  
Tatsushi Goto ◽  
Fumio Tokunaga ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Progenitor Cells ◽  
Annual Meeting ◽  
Retinal Progenitor Cells ◽  
Retinal Progenitor ◽  
Biophysical Society ◽  
Bhlh Transcription Factors

scholarly journals 2P-108 Analyses of recognition mechanism of E-box sequence by bHLH transcription factors(The 46th Annual Meeting of the Biophysical Society of Japan)

2008 ◽  
Vol 48 (supplement) ◽  
pp. S92
Author(s):  
Koji HASEGAWA ◽  
Tatsushi GOTO ◽  
Mari KOTOURA ◽  
Daisuke KITANO ◽  
Fumio TOKUNAGA ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Annual Meeting ◽  
Recognition Mechanism ◽  
E Box ◽  
Biophysical Society ◽  
Bhlh Transcription Factors

Long-Term in vitro Expansion of Retinal Progenitor Cells by Culturing Intact Neurospheres in Monolayer

2008 ◽  
Vol 40 (6) ◽  
pp. 291-297 ◽  
Author(s):  
Pinghong Lai ◽  
Shibo Tang ◽  
Jinglin Yi ◽  
Xiaobo Zhu ◽  
Yuping Zou ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Retinal Progenitor Cells ◽  
Retinal Progenitor ◽  
In Vitro Expansion

scholarly journals Construction and Characterization of EGFP Reporter Plasmid Harboring Putative Human RAX Promoter for in vitro Monitoring of Retinal Progenitor Cells Identity

2020 ◽  
Author(s):  
Atefeh Atefi ◽  
Pendar Shojaei kojouri ◽  
Fereshteh Karamali ◽  
Shiva Irani ◽  
Mohammad Hossein Nasr Esfahani
Keyword(s):  
Progenitor Cells ◽  
Molecular Mechanisms ◽  
Embryonic Stem ◽  
Retinal Layer ◽  
Retinal Progenitor Cells ◽  
Regulatory Regions ◽  
Retina Development ◽  
Retinal Progenitor ◽  
Egfp Reporter

Abstract Background : In retinal degenerative disease, progressive and debilitating conditions result in deterioration of retinal cells and visual loss. In human, retina lacks the inherent capacity for regeneration. Therefore, regeneration of retinal layer from human retinal progenitor cells (hRPCs) is a challenging task and restricted in vitro maintenance of hRPCs remains as the main hurdle. Retina and anterior neural fold homeobox gene ( RAX ) play critical roles in developing retina and maintenance of hRPCs. In this study, for the first time regulatory regions of human RAX gene with potential promoter activity were experimentally investigated. Results: For this purpose, after in silico analysis of regulatory regions of human RAX gene, the expression of EGFP reporter derived by putative promoter sequences was first evaluated in 293T cells and then in hRPCS derived from human embryonic stem cells. The candidate region ( RAX -3258bp) showed the highest EGFP expression in hRPCs. This reporter construct can be used for invitro monitoring of hRPC identity and verification of an efficient culture medium for maintenance of these cells. Conclusions: Furthermore, our findings provide a platform for better insight into regulatory regions of human RAX gene and molecular mechanisms underlying its vital functions in retina development.

An in vitro comparison of two different subpopulations of retinal progenitor cells for self-renewal and multipotentiality

2012 ◽  
Vol 1433 ◽  
pp. 38-46 ◽  
Author(s):  
Jing Xia ◽  
Hao Liu ◽  
Xianqun Fan ◽  
Yamin Hu ◽  
Yidan Zhang ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Retinal Progenitor Cells ◽  
Self Renewal ◽  
Retinal Progenitor ◽  
In Vitro Comparison

scholarly journals Revisiting nestin expression in retinal progenitor cells in vitro and after transplantation in vivo

2007 ◽  
Vol 84 (6) ◽  
pp. 1047-1059 ◽  
Author(s):  
Guanting Qiu ◽  
Magdalene J. Seiler ◽  
Biju B. Thomas ◽  
Kebin Wu ◽  
Michael Radosevich ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Retinal Progenitor Cells ◽  
Nestin Expression ◽  
Retinal Progenitor
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