scholarly journals Effects of let-7b and TLX on the proliferation and differentiation of retinal progenitor cells in vitro

2014 ◽  
Vol 4 (1) ◽  
Author(s):  
Ni Ni ◽  
Dandan Zhang ◽  
Qing Xie ◽  
Junzhao Chen ◽  
Zi Wang ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Retinal Progenitor Cells ◽  
Retinal Progenitor ◽  
Proliferation And Differentiation

scholarly journals Hyaluronan-CD44 Interaction Regulates Mouse Retinal Progenitor Cells Migration, Proliferation and Neuronal Differentiation

2021 ◽  
Author(s):  
Jian Ma ◽  
Xiaoyun Fang ◽  
Min Chen ◽  
Yao Wang ◽  
Li Zhang
Keyword(s):  
Western Blot ◽  
Progenitor Cells ◽  
Neuronal Differentiation ◽  
Collagen Gel ◽  
Time Lapse ◽  
Retinal Progenitor Cells ◽  
Western Blot Assay ◽  
Retinal Progenitor ◽  
Proliferation And Differentiation ◽  
And Migration

Abstract Background: Therapeutic applications of retinal progenitor cells (RPCs) are hindered by their limited proliferation and differentiation capacity and poor ability to migrate into damaged retinal tissue. Our study aimed to explore the effects of HA-CD44 interactions on the regulation of RPCs migration, proliferation and differentiation, and to investigate the underlying regulation mechanisms.Methods: Mouse RPCs were isolated and amplified. Western blot and flow cytometry analyses were used to investigate the expression of CD44 in RPCs. The effects of HA-CD44 interactions on the RPCs behaviors, including migration, proliferation and differentiation, were investigated by MTT assay, CCK8 assay, vertical collagen gel invasion assay, time-lapse imaging, immunocytochemistry, RT-PCR and western blot assay. Furthermore, the downstream signals of HA-CD44 interactions were investigated.Results: CD44 was expressed in RPCs, and HA-CD44 interaction markedly improved RPCs adhesion and migration. The stimulation of miR-21 expression by HA-CD44 interaction was PKC/Nanog-dependent in RPCs. Treatment of RPCs with PKC- or Nanog-specific ASODN or miR-21 antagomir effectively blocked HA-mediated RPCs adhesion and migration. Moreover, ROK/Gab-1 associated PI3K/AKT signaling activation was required in the HA-CD44 interaction mediated RPCs proliferation and neuronal differentiation.Conclusions: Our findings demonstrated new roles for HA-CD44 interaction in regulating both migration, proliferation and neuronal differentiation of RPCs. HA-CD44 signaling could comprise a novel approach to control RPC fates, which may be instructive for the application of RPCs for future therapeutic application.

Enhanced proliferation and differentiation of retinal progenitor cells through a self-healing injectable hydrogel

2019 ◽  
Vol 7 (6) ◽  
pp. 2335-2347 ◽  
Author(s):  
Fang Jiang ◽  
Zhimin Tang ◽  
Yuanhao Zhang ◽  
Yahan Ju ◽  
Huiqin Gao ◽  
...  
Keyword(s):  
Schiff Base ◽  
Progenitor Cells ◽  
Self Healing ◽  
Retinal Progenitor Cells ◽  
Injectable Hydrogel ◽  
Retinal Progenitor ◽  
Proliferation And Differentiation

Fabrication of self-healing injectable CS-Odex hydrogels via a dynamic Schiff-base linkage for RPC delivery.

scholarly journals miR-762 regulates the proliferation and differentiation of retinal progenitor cells by targeting NPDC1

Cell Cycle ◽  
2020 ◽  
Vol 19 (14) ◽  
pp. 1754-1767
Author(s):  
Huiqin Gao ◽  
Ni Ni ◽  
Dandan Zhang ◽  
Yuyao Wang ◽  
Zhimin Tang ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Retinal Progenitor Cells ◽  
Retinal Progenitor ◽  
Proliferation And Differentiation

Long-Term in vitro Expansion of Retinal Progenitor Cells by Culturing Intact Neurospheres in Monolayer

2008 ◽  
Vol 40 (6) ◽  
pp. 291-297 ◽  
Author(s):  
Pinghong Lai ◽  
Shibo Tang ◽  
Jinglin Yi ◽  
Xiaobo Zhu ◽  
Yuping Zou ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Retinal Progenitor Cells ◽  
Retinal Progenitor ◽  
In Vitro Expansion

scholarly journals Construction and Characterization of EGFP Reporter Plasmid Harboring Putative Human RAX Promoter for in vitro Monitoring of Retinal Progenitor Cells Identity

2020 ◽  
Author(s):  
Atefeh Atefi ◽  
Pendar Shojaei kojouri ◽  
Fereshteh Karamali ◽  
Shiva Irani ◽  
Mohammad Hossein Nasr Esfahani
Keyword(s):  
Progenitor Cells ◽  
Molecular Mechanisms ◽  
Embryonic Stem ◽  
Retinal Layer ◽  
Retinal Progenitor Cells ◽  
Regulatory Regions ◽  
Retina Development ◽  
Retinal Progenitor ◽  
Egfp Reporter

Abstract Background : In retinal degenerative disease, progressive and debilitating conditions result in deterioration of retinal cells and visual loss. In human, retina lacks the inherent capacity for regeneration. Therefore, regeneration of retinal layer from human retinal progenitor cells (hRPCs) is a challenging task and restricted in vitro maintenance of hRPCs remains as the main hurdle. Retina and anterior neural fold homeobox gene ( RAX ) play critical roles in developing retina and maintenance of hRPCs. In this study, for the first time regulatory regions of human RAX gene with potential promoter activity were experimentally investigated. Results: For this purpose, after in silico analysis of regulatory regions of human RAX gene, the expression of EGFP reporter derived by putative promoter sequences was first evaluated in 293T cells and then in hRPCS derived from human embryonic stem cells. The candidate region ( RAX -3258bp) showed the highest EGFP expression in hRPCs. This reporter construct can be used for invitro monitoring of hRPC identity and verification of an efficient culture medium for maintenance of these cells. Conclusions: Furthermore, our findings provide a platform for better insight into regulatory regions of human RAX gene and molecular mechanisms underlying its vital functions in retina development.

An in vitro comparison of two different subpopulations of retinal progenitor cells for self-renewal and multipotentiality

2012 ◽  
Vol 1433 ◽  
pp. 38-46 ◽  
Author(s):  
Jing Xia ◽  
Hao Liu ◽  
Xianqun Fan ◽  
Yamin Hu ◽  
Yidan Zhang ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Retinal Progenitor Cells ◽  
Self Renewal ◽  
Retinal Progenitor ◽  
In Vitro Comparison
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