Long-Term in vitro Expansion of Retinal Progenitor Cells by Culturing Intact Neurospheres in Monolayer

2008 ◽  
Vol 40 (6) ◽  
pp. 291-297 ◽  
Author(s):  
Pinghong Lai ◽  
Shibo Tang ◽  
Jinglin Yi ◽  
Xiaobo Zhu ◽  
Yuping Zou ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Retinal Progenitor Cells ◽  
Retinal Progenitor ◽  
In Vitro Expansion

scholarly journals Human Cord Blood-Derived AC133+ Progenitor Cells Preserve Endothelial Progenitor Characteristics after Long Term In Vitro Expansion

PLoS ONE ◽  
2010 ◽  
Vol 5 (2) ◽  
pp. e9173 ◽  
Author(s):  
Branislava Janic ◽  
Austin M. Guo ◽  
A. S. M. Iskander ◽  
Nadimpalli Ravi S. Varma ◽  
Alfonso G. Scicli ◽  
...  
Keyword(s):  
Cord Blood ◽  
Progenitor Cells ◽  
Endothelial Progenitor ◽  
Human Cord Blood ◽  
In Vitro Expansion

scholarly journals Construction and Characterization of EGFP Reporter Plasmid Harboring Putative Human RAX Promoter for in vitro Monitoring of Retinal Progenitor Cells Identity

2020 ◽  
Author(s):  
Atefeh Atefi ◽  
Pendar Shojaei kojouri ◽  
Fereshteh Karamali ◽  
Shiva Irani ◽  
Mohammad Hossein Nasr Esfahani
Keyword(s):  
Progenitor Cells ◽  
Molecular Mechanisms ◽  
Embryonic Stem ◽  
Retinal Layer ◽  
Retinal Progenitor Cells ◽  
Regulatory Regions ◽  
Retina Development ◽  
Retinal Progenitor ◽  
Egfp Reporter

Abstract Background : In retinal degenerative disease, progressive and debilitating conditions result in deterioration of retinal cells and visual loss. In human, retina lacks the inherent capacity for regeneration. Therefore, regeneration of retinal layer from human retinal progenitor cells (hRPCs) is a challenging task and restricted in vitro maintenance of hRPCs remains as the main hurdle. Retina and anterior neural fold homeobox gene ( RAX ) play critical roles in developing retina and maintenance of hRPCs. In this study, for the first time regulatory regions of human RAX gene with potential promoter activity were experimentally investigated. Results: For this purpose, after in silico analysis of regulatory regions of human RAX gene, the expression of EGFP reporter derived by putative promoter sequences was first evaluated in 293T cells and then in hRPCS derived from human embryonic stem cells. The candidate region ( RAX -3258bp) showed the highest EGFP expression in hRPCs. This reporter construct can be used for invitro monitoring of hRPC identity and verification of an efficient culture medium for maintenance of these cells. Conclusions: Furthermore, our findings provide a platform for better insight into regulatory regions of human RAX gene and molecular mechanisms underlying its vital functions in retina development.

An in vitro comparison of two different subpopulations of retinal progenitor cells for self-renewal and multipotentiality

2012 ◽  
Vol 1433 ◽  
pp. 38-46 ◽  
Author(s):  
Jing Xia ◽  
Hao Liu ◽  
Xianqun Fan ◽  
Yamin Hu ◽  
Yidan Zhang ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Retinal Progenitor Cells ◽  
Self Renewal ◽  
Retinal Progenitor ◽  
In Vitro Comparison

scholarly journals Revisiting nestin expression in retinal progenitor cells in vitro and after transplantation in vivo

2007 ◽  
Vol 84 (6) ◽  
pp. 1047-1059 ◽  
Author(s):  
Guanting Qiu ◽  
Magdalene J. Seiler ◽  
Biju B. Thomas ◽  
Kebin Wu ◽  
Michael Radosevich ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Retinal Progenitor Cells ◽  
Nestin Expression ◽  
Retinal Progenitor

scholarly journals Human Retinal Progenitor Cells Derived Small Extracellular Vesicles Delay Retinal Degeneration: A Paradigm for Cell-free Therapy

2021 ◽  
Vol 12 ◽  
Author(s):  
Min Chen ◽  
Chunge Ren ◽  
Bangqi Ren ◽  
Yajie Fang ◽  
Qiyou Li ◽  
...  
Keyword(s):  
Retinal Degeneration ◽  
Progenitor Cells ◽  
Extracellular Vesicles ◽  
Target Genes ◽  
Outer Nuclear Layer ◽  
Subretinal Space ◽  
Retinal Progenitor Cells ◽  
Retinal Progenitor ◽  
Rcs Rats

Retinal degeneration is a leading cause of irreversible vision impairment and blindness worldwide. Previous studies indicate that subretinal injection of human retinal progenitor cells (hRPCs) can delay the progression of retinal degeneration, preserve retinal function, and protect photoreceptor cells from death, albeit the mechanism is not well understood. In this study, small extracellular vesicles derived from hRPCs (hRPC-sEVs) were injected into the subretinal space of retinal dystrophic RCS rats. We find that hRPC-sEVs significantly preserve the function of retina and thickness of the outer nuclear layer (ONL), reduce the apoptosis of photoreceptors in the ONL, and suppress the inflammatory response in the retina of RCS rats. In vitro, we have shown that hRPC-sEV treatment could significantly reserve the low-glucose preconditioned apoptosis of photoreceptors and reduce the expression of pro-inflammatory cytokines in microglia. Pathway analysis predicted the target genes of hRPC-sEV microRNAs involved in inflammation related biological processes and significantly enriched in processes autophagy, signal release, regulation of neuron death, and cell cycle. Collectively, our study suggests that hRPC-sEVs might be a favorable agent to delay retinal degeneration and highlights as a new paradigm for cell-free therapy.

Comparison of murine dental follicle precursor and retinal progenitor cells after neural differentiation in vitro

2009 ◽  
Vol 33 (7) ◽  
pp. 758-764 ◽  
Author(s):  
Wolfgang Ernst ◽  
Michael Saugspier ◽  
Oliver Felthaus ◽  
Oliver Driemel ◽  
Christian Morsczeck
Keyword(s):  
Progenitor Cells ◽  
Neural Differentiation ◽  
Dental Follicle ◽  
Retinal Progenitor Cells ◽  
Retinal Progenitor
Sign in / Sign up

Export Citation Format

Share Document