A Diagnostic Platform for Rapid, Simultaneous Quantification of Procalcitonin and C-Reactive Protein in Human Serum

2021 ◽  
Author(s):  
Xiangkun Cao ◽  
Ongagna Y. Serge Yvon ◽  
Ruisheng Wang ◽  
Yue Ren ◽  
Balaji Srinivasan ◽  
...  
Keyword(s):  
Human Serum ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Simultaneous Quantification

scholarly journals Interactions of C-reactive protein with the complement system. III. Complement-dependent passive hemolysis initiated by CRP.

1975 ◽  
Vol 142 (5) ◽  
pp. 1065-1077 ◽  
Author(s):  
A.P. Osmand ◽  
R.F. Mortensen ◽  
Joan Siegel ◽  
H. Gewurz
Keyword(s):  
Guinea Pig ◽  
Human Serum ◽  
Complete System ◽  
Gel Filtration ◽  
C Reactive Protein ◽  
Inflammatory Reactions ◽  
Reactive Protein ◽  
Rabbit Igg ◽  
The Complement System ◽  
Pig Serum

Interactions of CRP with various substrates in the presence of human serum have been shown to result in efficient activation of C components C1-C5. We now report the ability of CRP to initiate C-dependent hemolysis. For this purpose CRP was isolated by affinity chromatography using pneumococcal CPS and gel filtration; its purity was established by several criteria. Erythrocytes were coated with CPS (E-CPS) and passively sensitized with CRP. C-dependent lysis of these cells was observed upon the addition of suitably absorbed human serum, and the efficiency of hemolysis compared favorably with that initiated by rabbit IgG anti-CPS antibody. CRP also sensitized E-CPS for lysis by guinea pig C; partial lysis was seen when C4-deficient guinea pig serum was used, suggesting that CRP also shares with antibody the ability of CRP to fully activate the C system and provide further evidence for a role for CRP similar to that of antibody in the initiation and modulation of inflammatory reactions via the complete system.

scholarly journals Phosphoethanolamine-complexed C-reactive protein: A pharmacological-like macromolecule that binds to native low-density lipoprotein in human serum

2008 ◽  
Vol 394 (1-2) ◽  
pp. 94-98 ◽  
Author(s):  
Sanjay K. Singh ◽  
Madathilparambil V. Suresh ◽  
Deborah C. Prayther ◽  
Jonathan P. Moorman ◽  
Antonio E. Rusiñol ◽  
...  
Keyword(s):  
Human Serum ◽  
Low Density Lipoprotein ◽  
Protein A ◽  
Density Lipoprotein ◽  
Low Density ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Native Low Density Lipoprotein

scholarly journals High sensitivity C-reactive protein (Hs-CRP) remains highly stable in long-term archived human serum

2014 ◽  
Vol 47 (4-5) ◽  
pp. 315-318 ◽  
Author(s):  
Ayo P. Doumatey ◽  
Jie Zhou ◽  
Adebowale Adeyemo ◽  
Charles Rotimi
Keyword(s):  
Human Serum ◽  
High Sensitivity ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Hs Crp

scholarly journals Changes in Human Serum Amyloid A and C-Reactive Protein after Etiocholanolone-Induced Inflammation

1978 ◽  
Vol 61 (2) ◽  
pp. 390-394 ◽  
Author(s):  
Keith P. W. J. McAdam ◽  
Ronald J. Elin ◽  
Jean D. Sipe ◽  
Sheldon M. Wolff
Keyword(s):  
Human Serum ◽  
Serum Amyloid A ◽  
Serum Amyloid ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Amyloid A

scholarly journals Electrochemical Detection of C-Reactive Protein in Human Serum Based on Self-Assembled Monolayer-Modified Interdigitated Wave-Shaped Electrode

Sensors ◽  
2019 ◽  
Vol 19 (24) ◽  
pp. 5560 ◽  
Author(s):  
Somasekhar R. Chinnadayyala ◽  
Jinsoo Park ◽  
Young Hyo Kim ◽  
Seong Hye Choi ◽  
Sang-Myung Lee ◽  
...  
Keyword(s):  
Human Serum ◽  
Electrochemical Impedance ◽  
Electrode Array ◽  
C Reactive Protein ◽  
Self Assembled Monolayer ◽  
X Ray ◽  
Reactive Protein ◽  
Relative Standard ◽  
Label Free Detection ◽  
Self Assembled

An electrochemical capacitance immunosensor based on an interdigitated wave-shaped micro electrode array (IDWµE) for direct and label-free detection of C-reactive protein (CRP) was reported. A self-assembled monolayer (SAM) of dithiobis (succinimidyl propionate) (DTSP) was used to modify the electrode array for antibody immobilization. The SAM functionalized electrode array was characterized morphologically by atomic force microscopy (AFM) and energy dispersive X-ray spectroscopy (EDX). The nature of gold-sulfur interactions on SAM-treated electrode array was probed by X-ray photoelectron spectroscopy (XPS). The covalent linking of anti-CRP-antibodies onto the SAM modified electrode array was characterized morphologically through AFM, and electrochemically through cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The application of phosphate-buffered saline (PBS) and human serum (HS) samples containing different concentrations of CRP in the electrode array caused changes in the electrode interfacial capacitance upon CRP binding. CRP concentrations in PBS and HS were determined quantitatively by measuring the change in capacitance (ΔC) through EIS. The electrode immobilized with anti-CRP-antibodies showed an increase in ΔC with the addition of CRP concentrations over a range of 0.01–10,000 ng mL−1. The electrode showed detection limits of 0.025 ng mL−1 and 0.23 ng mL−1 (S/N = 3) in PBS and HS, respectively. The biosensor showed a good reproducibility (relative standard deviation (RSD), 1.70%), repeatability (RSD, 1.95%), and adequate selectivity in presence of interferents towards CRP detection. The sensor also exhibited a significant storage stability of 2 weeks at 4 °C in 1× PBS.

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