Microfluidic Capillary System for Immunoaffinity Separations of C-Reactive Protein in Human Serum and Cerebrospinal Fluid

2008 ◽  
Vol 80 (10) ◽  
pp. 3853-3858 ◽  
Author(s):  
Michael C. Peoples ◽  
H. Thomas Karnes
Keyword(s):  
Cerebrospinal Fluid ◽  
Human Serum ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Capillary System

scholarly journals Interactions of C-reactive protein with the complement system. III. Complement-dependent passive hemolysis initiated by CRP.

1975 ◽  
Vol 142 (5) ◽  
pp. 1065-1077 ◽  
Author(s):  
A.P. Osmand ◽  
R.F. Mortensen ◽  
Joan Siegel ◽  
H. Gewurz
Keyword(s):  
Guinea Pig ◽  
Human Serum ◽  
Complete System ◽  
Gel Filtration ◽  
C Reactive Protein ◽  
Inflammatory Reactions ◽  
Reactive Protein ◽  
Rabbit Igg ◽  
The Complement System ◽  
Pig Serum

Interactions of CRP with various substrates in the presence of human serum have been shown to result in efficient activation of C components C1-C5. We now report the ability of CRP to initiate C-dependent hemolysis. For this purpose CRP was isolated by affinity chromatography using pneumococcal CPS and gel filtration; its purity was established by several criteria. Erythrocytes were coated with CPS (E-CPS) and passively sensitized with CRP. C-dependent lysis of these cells was observed upon the addition of suitably absorbed human serum, and the efficiency of hemolysis compared favorably with that initiated by rabbit IgG anti-CPS antibody. CRP also sensitized E-CPS for lysis by guinea pig C; partial lysis was seen when C4-deficient guinea pig serum was used, suggesting that CRP also shares with antibody the ability of CRP to fully activate the C system and provide further evidence for a role for CRP similar to that of antibody in the initiation and modulation of inflammatory reactions via the complete system.

scholarly journals Phosphoethanolamine-complexed C-reactive protein: A pharmacological-like macromolecule that binds to native low-density lipoprotein in human serum

2008 ◽  
Vol 394 (1-2) ◽  
pp. 94-98 ◽  
Author(s):  
Sanjay K. Singh ◽  
Madathilparambil V. Suresh ◽  
Deborah C. Prayther ◽  
Jonathan P. Moorman ◽  
Antonio E. Rusiñol ◽  
...  
Keyword(s):  
Human Serum ◽  
Low Density Lipoprotein ◽  
Protein A ◽  
Density Lipoprotein ◽  
Low Density ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Native Low Density Lipoprotein

scholarly journals Acute childhood encephalopathy with prolonged fever, pleocytosis and elevated inflammatory cytokines in the cerebrospinal fluid and transient splenial lesion

2019 ◽  
Author(s):  
Rena Okada ◽  
Yuri Sakaguchi ◽  
Takeshi Matsushige ◽  
Isamu Kamimaki ◽  
Toshiki Takenouchi ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Mental Status ◽  
Nationwide Survey ◽  
Altered Mental Status ◽  
Acute Encephalopathy ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Prolonged Fever ◽  
Splenial Lesion ◽  
Male Patients

Background: Acute encephalopathy during childhood represents a highly heterogeneous group of infectious and non-infectious pathologies. According to a recent nationwide survey on acute childhood encephalopathy in Japan, the combination of clinical and radiographic features left approximately half of the affected children unclassified, mainly because of the lack of disease-specific biomarkers. Case: Herein, we document a school-aged boy who manifested with acute encephalopathy that was characterized by a prolonged fever, altered mental status, urinary retention, and intention tremor lasting for more than a month. Accompanying features included syndrome of inappropriate secretion of antidiuretic hormone, pleocytosis with elevated interleukin-6 and interferon-gamma levels in the cerebrospinal fluid, and a transient splenial lesion on neuroimaging. No pathogens were identified, and C-reactive protein was negative throughout his clinical course. This constellation of clinical features was not compatible with any of the existing entities of acute pediatric encephalopathy. Discussion: Our retrospective literature review identified two additional school-aged male patients who exhibited highly similar clinical courses. The prolonged altered mental status with pleocytosis in the cerebrospinal fluid and a transient splenial lesion in the absence of serum inflammatory markers suggest a primary central nervous system pathology. Conclusion: This combination of features defines this presumably new group of acute childhood febrile encephalopathy with prolonged fever and ataxia in school-aged boys.

A Diagnostic Platform for Rapid, Simultaneous Quantification of Procalcitonin and C-Reactive Protein in Human Serum

2021 ◽  
Author(s):  
Xiangkun Cao ◽  
Ongagna Y. Serge Yvon ◽  
Ruisheng Wang ◽  
Yue Ren ◽  
Balaji Srinivasan ◽  
...  
Keyword(s):  
Human Serum ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Simultaneous Quantification

scholarly journals C - reactive protein of cerebrospinal fluid, as a sensitive approach for diagnosis of neonatal meningitis

2019 ◽  
Vol 19 (3) ◽  
pp. 2372-2377
Author(s):  
Shima Javadinia ◽  
Mohsen Tabasi ◽  
Mehri Naghdalipour ◽  
Najmosadat Atefi ◽  
Ramin Asgarian ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Aseptic Meningitis ◽  
Great Majority ◽  
Diagnostic Value ◽  
Latex Agglutination ◽  
Neonatal Meningitis ◽  
C Reactive Protein ◽  
Cross Sectional ◽  
Reactive Protein ◽  
Permanent Brain Damage

Background: Meningitis, is a potentially life-threatening condition that can rapidly progress to permanent brain damage, neurologic problems, and even death. Bacteria and viruses cause the great majority of meningitis disease in infants and children. CRP is used mainly as a marker of inflammation.Objective: This study was conducted to assess the diagnostic value of CSF-CRP levels for differentiating between septic (bacterial) and aseptic infantile meningitis.Methods: 49 hospitalized infants aged less than two months with suspected meningitis were enrolled in a cross-sectional analytic study. All of patients underwent lumbar puncture to obtain CSF. smears, cultures, cytological and biochemical analysis and latex agglutination testing were carried out on all CSF samples. Latex agglutination test was carried out on all CSF samples using a commercially available kit. CSF-CRP level of all infants was measured using the immunoturbidometric technique.Results: Of 49 infants in this study, 20 and 29 cases were diagnosed as septic and aseptic meningitis, respectively. The CRP levels were obtained as 0.95±0.68 mg/L in septic and 0.16±0.36 mg/L in aseptic meningitis groups and this difference was statistically significant (p<0.001) between the two groups (0.79±0.32 mg/L). Based on the ROC curve, cut off levels for CRP was obtained 0.17 mg/L. At this level, there was 95% sensitivity and 86% specificity to differentiate septic and aseptic meningitis.Conclusion: CSF-CRP has suitable diagnostic value in distinguishing between infantile bacterial from aseptic meningitis especially in cases of negative bacterial culture of the blood and spinal fluid.Keywords: C-reactive protein, cerebrospinal fluid, septic/aseptic meningitis, infant, diagnostic value.

C-reactive protein in serum and cerebrospinal fluid in various neurological disorders

1984 ◽  
Vol 63 (3) ◽  
pp. 339-344 ◽  
Author(s):  
C.J.M Sindic ◽  
D Collet-Cassart ◽  
A Depré ◽  
E.C Laterre ◽  
P.L Masson
Keyword(s):  
Cerebrospinal Fluid ◽  
Neurological Disorders ◽  
C Reactive Protein ◽  
Reactive Protein

scholarly journals 1104 QUANTITATIVE MEASUREMENT OF C-REACTIVE PROTEIN (CRP) IN CEREBROSPINAL FLUID (CSF) OF PATIENTS WITH BACTERIAL MENINGITIS

1985 ◽  
Vol 19 (4) ◽  
pp. 294A-294A
Author(s):  
Barry M Gray ◽  
Don Simmons ◽  
Henry F Mason ◽  
Scott K Barnum ◽  
John E Volanakis
Keyword(s):  
Cerebrospinal Fluid ◽  
Bacterial Meningitis ◽  
Quantitative Measurement ◽  
C Reactive Protein ◽  
Reactive Protein

scholarly journals High sensitivity C-reactive protein (Hs-CRP) remains highly stable in long-term archived human serum

2014 ◽  
Vol 47 (4-5) ◽  
pp. 315-318 ◽  
Author(s):  
Ayo P. Doumatey ◽  
Jie Zhou ◽  
Adebowale Adeyemo ◽  
Charles Rotimi
Keyword(s):  
Human Serum ◽  
High Sensitivity ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Hs Crp
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