scholarly journals Electrochemical Detection of C-Reactive Protein in Human Serum Based on Self-Assembled Monolayer-Modified Interdigitated Wave-Shaped Electrode

Sensors ◽  
2019 ◽  
Vol 19 (24) ◽  
pp. 5560 ◽  
Author(s):  
Somasekhar R. Chinnadayyala ◽  
Jinsoo Park ◽  
Young Hyo Kim ◽  
Seong Hye Choi ◽  
Sang-Myung Lee ◽  
...  
Keyword(s):  
Human Serum ◽  
Electrochemical Impedance ◽  
Electrode Array ◽  
C Reactive Protein ◽  
Self Assembled Monolayer ◽  
X Ray ◽  
Reactive Protein ◽  
Relative Standard ◽  
Label Free Detection ◽  
Self Assembled

An electrochemical capacitance immunosensor based on an interdigitated wave-shaped micro electrode array (IDWµE) for direct and label-free detection of C-reactive protein (CRP) was reported. A self-assembled monolayer (SAM) of dithiobis (succinimidyl propionate) (DTSP) was used to modify the electrode array for antibody immobilization. The SAM functionalized electrode array was characterized morphologically by atomic force microscopy (AFM) and energy dispersive X-ray spectroscopy (EDX). The nature of gold-sulfur interactions on SAM-treated electrode array was probed by X-ray photoelectron spectroscopy (XPS). The covalent linking of anti-CRP-antibodies onto the SAM modified electrode array was characterized morphologically through AFM, and electrochemically through cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The application of phosphate-buffered saline (PBS) and human serum (HS) samples containing different concentrations of CRP in the electrode array caused changes in the electrode interfacial capacitance upon CRP binding. CRP concentrations in PBS and HS were determined quantitatively by measuring the change in capacitance (ΔC) through EIS. The electrode immobilized with anti-CRP-antibodies showed an increase in ΔC with the addition of CRP concentrations over a range of 0.01–10,000 ng mL−1. The electrode showed detection limits of 0.025 ng mL−1 and 0.23 ng mL−1 (S/N = 3) in PBS and HS, respectively. The biosensor showed a good reproducibility (relative standard deviation (RSD), 1.70%), repeatability (RSD, 1.95%), and adequate selectivity in presence of interferents towards CRP detection. The sensor also exhibited a significant storage stability of 2 weeks at 4 °C in 1× PBS.

scholarly journals An Electrochemical Immunosensor Based on a Self-Assembled Monolayer Modified Electrode for Label-Free Detection of α-Synuclein

Sensors ◽  
2020 ◽  
Vol 20 (3) ◽  
pp. 617 ◽  
Author(s):  
Chuang-Ye Ge ◽  
Md. Mahbubur Rahman ◽  
Wei Zhang ◽  
Nasrin Siraj Lopa ◽  
Lei Jin ◽  
...  
Keyword(s):  
Dynamic Range ◽  
Electrochemical Impedance ◽  
Charge Transfer Resistance ◽  
Label Free ◽  
Serum Samples ◽  
Self Assembled Monolayer ◽  
Electrochemical Impedance Spectra ◽  
Transfer Resistance ◽  
Label Free Detection ◽  
Self Assembled

This research demonstrated the development of a simple, cost-effective, and label-free immunosensor for the detection of α-synuclein (α-Syn) based on a cystamine (CYS) self-assembled monolayer (SAM) decorated fluorine-doped tin oxide (FTO) electrode. CYS-SAM was formed onto the FTO electrode by the adsorption of CYS molecules through the head sulfur groups. The free amine (–NH2) groups at the tail of the CYS-SAM enabled the immobilization of anti-α-Syn-antibody, which concurrently allowed the formation of immunocomplex by covalent bonding with α-Syn-antigen. The variation of the concentrations of the attached α-Syn at the immunosensor probe induced the alternation of the current and the charge transfer resistance (Rct) for the redox response of [Fe(CN)6]3−/4−, which displayed a linear dynamic range from 10 to 1000 ng/mL with a low detection limit (S/N = 3) of ca. 3.62 and 1.13 ng/mL in differential pulse voltammetry (DPV) and electrochemical impedance spectra (EIS) measurements, respectively. The immunosensor displayed good reproducibility, anti-interference ability, and good recoveries of α-Syn detection in diluted human serum samples. The proposed immunosensor is a promising platform to detect α-Syn for the early diagnose of Parkinson’s disease, which can be extended for the determination of other biologically important biomarkers.

Detection of C-reactive protein on a functional poly(thiophene) self-assembled monolayer using surface plasmon resonance

2008 ◽  
Vol 108 (10) ◽  
pp. 1379-1383 ◽  
Author(s):  
Hyun-Chul Kim ◽  
Soo-Keun Lee ◽  
Won Bae Jeon ◽  
Hong-Kun Lyu ◽  
Seung Woo Lee ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
C Reactive Protein ◽  
Self Assembled Monolayer ◽  
Reactive Protein ◽  
Self Assembled

scholarly journals Interactions of C-reactive protein with the complement system. III. Complement-dependent passive hemolysis initiated by CRP.

1975 ◽  
Vol 142 (5) ◽  
pp. 1065-1077 ◽  
Author(s):  
A.P. Osmand ◽  
R.F. Mortensen ◽  
Joan Siegel ◽  
H. Gewurz
Keyword(s):  
Guinea Pig ◽  
Human Serum ◽  
Complete System ◽  
Gel Filtration ◽  
C Reactive Protein ◽  
Inflammatory Reactions ◽  
Reactive Protein ◽  
Rabbit Igg ◽  
The Complement System ◽  
Pig Serum

Interactions of CRP with various substrates in the presence of human serum have been shown to result in efficient activation of C components C1-C5. We now report the ability of CRP to initiate C-dependent hemolysis. For this purpose CRP was isolated by affinity chromatography using pneumococcal CPS and gel filtration; its purity was established by several criteria. Erythrocytes were coated with CPS (E-CPS) and passively sensitized with CRP. C-dependent lysis of these cells was observed upon the addition of suitably absorbed human serum, and the efficiency of hemolysis compared favorably with that initiated by rabbit IgG anti-CPS antibody. CRP also sensitized E-CPS for lysis by guinea pig C; partial lysis was seen when C4-deficient guinea pig serum was used, suggesting that CRP also shares with antibody the ability of CRP to fully activate the C system and provide further evidence for a role for CRP similar to that of antibody in the initiation and modulation of inflammatory reactions via the complete system.

scholarly journals Microfluidic Impedance Biosensor Chips Using Sensing Layers Based on DNA-Based Self-Assembled Monolayers for Label-Free Detection of Proteins

Biosensors ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 80
Author(s):  
Khaled Alsabbagh ◽  
Tim Hornung ◽  
Achim Voigt ◽  
Sahba Sadir ◽  
Taleieh Rajabi ◽  
...  
Keyword(s):  
Troponin I ◽  
Electrochemical Impedance ◽  
Specific Binding ◽  
Self Assembled Monolayers ◽  
Significant Shift ◽  
Label Free ◽  
Label Free Detection ◽  
Assembled Monolayers ◽  
Self Assembled ◽  
Biosensor Chip

A microfluidic chip for electrochemical impedance spectroscopy (EIS) is presented as bio-sensor for label-free detection of proteins by using the example of cardiac troponin I. Troponin I is one of the most specific diagnostic serum biomarkers for myocardial infarction. The microfluidic impedance biosensor chip presented here consists of a microscope glass slide serving as base plate, sputtered electrodes, and a polydimethylsiloxane (PDMS) microchannel. Electrode functionalization protocols were developed considering a possible charge transfer through the sensing layer, in addition to analyte-specific binding by corresponding antibodies and reduction of nonspecific protein adsorption to prevent false-positive signals. Reagents tested for self-assembled monolayers (SAMs) on gold electrodes included thiolated hydrocarbons and thiolated oligonucleotides, where SAMs based on the latter showed a better performance. The corresponding antibody was covalently coupled on the SAM using carbodiimide chemistry. Sampling and measurement took only a few minutes. Application of a human serum albumin (HSA) sample, 1000 ng/mL, led to negligible impedance changes, while application of a troponin I sample, 1 ng/mL, led to a significant shift in the Nyquist plot. The results are promising regarding specific detection of clinically relevant concentrations of biomarkers, such as cardiac markers, with the newly developed microfluidic impedance biosensor chip.

Label-free detection of C-reactive protein using reflectometric interference spectroscopy-based sensing system

2012 ◽  
Vol 728 ◽  
pp. 64-68 ◽  
Author(s):  
Hyung Woo Choi ◽  
Yasuhiko Sakata ◽  
Yoshikazu Kurihara ◽  
Tooru Ooya ◽  
Toshifumi Takeuchi
Keyword(s):  
Label Free ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Sensing System ◽  
Reflectometric Interference Spectroscopy ◽  
Label Free Detection

Label-free detection of C-Reactive protein using bioresponsive hydrogel-based surface relief diffraction gratings

2021 ◽  
pp. 113561
Author(s):  
María Isabel Lucío ◽  
Andy Hernández Montoto ◽  
Estrella Fernández ◽  
Sabri Alamri ◽  
Tim Kunze ◽  
...  
Keyword(s):  
Surface Relief ◽  
Diffraction Gratings ◽  
Label Free ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Label Free Detection

scholarly journals Phosphoethanolamine-complexed C-reactive protein: A pharmacological-like macromolecule that binds to native low-density lipoprotein in human serum

2008 ◽  
Vol 394 (1-2) ◽  
pp. 94-98 ◽  
Author(s):  
Sanjay K. Singh ◽  
Madathilparambil V. Suresh ◽  
Deborah C. Prayther ◽  
Jonathan P. Moorman ◽  
Antonio E. Rusiñol ◽  
...  
Keyword(s):  
Human Serum ◽  
Low Density Lipoprotein ◽  
Protein A ◽  
Density Lipoprotein ◽  
Low Density ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Native Low Density Lipoprotein

Pattern formation using polystyrene benzaldimine self-assembled monolayer by soft X-ray

2018 ◽  
Vol 51 (4) ◽  
pp. 408-412
Author(s):  
Chandrashekhar Channapura Halappa ◽  
So-Jin Park
Keyword(s):  
Pattern Formation ◽  
Self Assembled Monolayer ◽  
X Ray ◽  
Self Assembled

A Diagnostic Platform for Rapid, Simultaneous Quantification of Procalcitonin and C-Reactive Protein in Human Serum

2021 ◽  
Author(s):  
Xiangkun Cao ◽  
Ongagna Y. Serge Yvon ◽  
Ruisheng Wang ◽  
Yue Ren ◽  
Balaji Srinivasan ◽  
...  
Keyword(s):  
Human Serum ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Simultaneous Quantification
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