A Microfluidic Chip Integrated with a Flexible CNT-PDMS Electrode Array for Simultaneous Detection of Cd 2+, Pb 2+, Hg 2+

2021 ◽  
Author(s):  
Wenhong Zhang ◽  
Zhipeng Xu ◽  
Li Wang ◽  
Weiguang Su ◽  
Xiangxiang Zhou ◽  
...  
Keyword(s):  
Microfluidic Chip ◽  
Simultaneous Detection ◽  
Electrode Array

scholarly journals Wave-shaped microfluidic chip assisted point-of-care testing for accurate and rapid diagnosis of infections

2021 ◽  
Author(s):  
Binfeng Yin ◽  
Xinhua Wan ◽  
Mingzhu Yang ◽  
Changcheng Qian ◽  
A S M Muhtasim Fuad Sohan
Keyword(s):  
Microfluidic Chip ◽  
Limit Of Detection ◽  
Point Of Care ◽  
Simultaneous Detection ◽  
Accurate Diagnosis ◽  
Point Of Care Testing ◽  
Promising Alternative ◽  
Reactive Protein ◽  
Linear Relationships ◽  
Multiple Biomarkers

Abstract Background: Simultaneous and timely detection of C-reactive protein (CRP), procalcitonin (PCT), and interleukin-6 (IL-6) provides effective information for the accurate diagnosis of infections. Early diagnosis and classification of infections increase the cure rate while decreasing complications, which is significant for severe infections, especially for war surgery. However, traditional methods rely on laborious operations and bulky devices. On the other hand, point-of-care (POC) methods suffer from limited robustness and accuracy. Therefore, it is of urgent demand to develop POC devices for rapid and accurate diagnosis of infections to fulfill on-site militarized requirements.Methods: We developed a wave-shaped microfluidic chip (WMC) assisted multiplexed detection platform (WMC-MDP). WMC-MDP reduces detection time and improves repeatability through premixing of the samples and reaction of the reagents. We further combined the detection platform with the streptavidin-biotin (SA-B) amplified system to enhance the sensitivity while using chemiluminescence (CL) intensity as signal readout. We realized simultaneous detection of CRP, PCT, and IL-6 on the detection platform and evaluated the sensitivity, linear range, selectivity, and repeatability. Finally, we finished detecting 15 samples from volunteers and compared the results with commercial ELISA kits.Results: Detection of CRP, PCT, and IL-6 exhibited good linear relationships between CL intensities and concentrations in the range of 1.25-40 μg/mL, 0.4-12.8 ng/mL, and 50-1600 pg/mL. The limit of detection (LOD) of CRP, PCT, and IL-6 were 0.54 μg/mL, 0.11 ng/mL, and 16.25 pg/mL, respectively. WMC-MDP is capable of good adequate selectivity and repeatability. The whole detection procedure takes only 22 minutes that meets the requirements of a POC device. Results of 15 samples from volunteers were consistent with the results detected by commercial ELISA kits.Conclusion: WMC-MDP allows simultaneous, rapid, and sensitive detection of CRP, PCT, and IL-6 with satisfactory selectivity and repeatability, requiring minimal manipulation. However, WMC-MDP takes advantage of being a microfluidic device showing the coefficients of variation less than 10% enabling WMC-MDP to be a type of POCT. Therefore, WMC-MDP provides a promising alternative to point-of-care testing (POCT) of multiple biomarkers. We believe the practical application of WMC-MDP in militarized fields will revolutionize infection diagnosis for soldiers.

scholarly journals Simultaneous detection of multiple HPV DNA via bottom-well microfluidic chip within an infra-red PCR platform

2018 ◽  
Vol 12 (2) ◽  
pp. 024109 ◽  
Author(s):  
Wenjia Liu ◽  
Antony Warden ◽  
Jiahui Sun ◽  
Guangxia Shen ◽  
Xianting Ding
Keyword(s):  
Microfluidic Chip ◽  
Simultaneous Detection ◽  
Hpv Dna ◽  
Infra Red

A portable microfluidic chip system for cancer diagnosis with simultaneous detection methods

2012 ◽  
Author(s):  
Hyoungseon Choi ◽  
Kwang Bok Kim ◽  
Changsu Jun ◽  
Taek Dong Chung ◽  
Hee Chan Kim
Keyword(s):  
Cancer Diagnosis ◽  
Microfluidic Chip ◽  
Simultaneous Detection ◽  
Detection Methods

Cell Imaging via Multi-Electrode Array Using Microfluidic Chip

2019 ◽  
Vol 9 (6) ◽  
pp. 1267-1271
Author(s):  
Dayong Fan ◽  
Jiafeng Yao
Keyword(s):  
Electrical Impedance Tomography ◽  
Microfluidic Chip ◽  
Electrical Impedance ◽  
Material Selection ◽  
Electrode Array ◽  
Cell Number ◽  
Structure Design ◽  
Cell Detection ◽  
Impedance Tomography ◽  
Multi Electrode Array

As a new medical detection method, electrical impedance tomography has been used in biology and medicine in recent years due to its advantages such as safety, non-invasiveness and radiation-free. In this paper, we designed a multi-electrode array microfluidic chip for the cell detection with electrical impedance tomography. The microfluidic chip structure design, material selection and processing technology are emphatically described. Finally, the feasibility of microfluidic chip for the cell with electrical impedance tomography is verified by simulations with different cell number and different electrodes excitation pattern.

Microfluidic chip coupled with optical biosensors for simultaneous detection of multiple analytes: A review

2019 ◽  
Vol 126 ◽  
pp. 697-706 ◽  
Author(s):  
Zerong Liao ◽  
Yang Zhang ◽  
Yongrui Li ◽  
Yunfei Miao ◽  
Shimeng Gao ◽  
...  
Keyword(s):  
Microfluidic Chip ◽  
Simultaneous Detection ◽  
Optical Biosensors ◽  
Multiple Analytes
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