A portable microfluidic chip system for cancer diagnosis with simultaneous detection methods

2012 ◽  
Author(s):  
Hyoungseon Choi ◽  
Kwang Bok Kim ◽  
Changsu Jun ◽  
Taek Dong Chung ◽  
Hee Chan Kim
Keyword(s):  
Cancer Diagnosis ◽  
Microfluidic Chip ◽  
Simultaneous Detection ◽  
Detection Methods

Research activities for the monitoring of genetically modified organisms in Japan

2010 ◽  
Vol 82 (1) ◽  
pp. 139-147
Author(s):  
Kazumi Kitta
Keyword(s):  
Quality Control ◽  
Genetically Modified Organisms ◽  
Genetically Modified ◽  
Screening Method ◽  
Simultaneous Detection ◽  
Cauliflower Mosaic Virus ◽  
Detection Methods ◽  
Internal Quality ◽  
Research Activities ◽  
Labeling System

The Japanese government introduced a labeling system for genetically modified (GM) foods. To ensure the authenticity of the labeling system, we have developed and validated detection methods for newly approved GM events. One was the development of quantitative analytical methods utilizing plasmid DNAs as calibrators, which enabled us to obtain an unlimited supply of calibrators of consistent quality and also to obtain a stable standard curve to quantify GM organisms (GMOs) in samples. The significance of quality control has been recognized among relevant stakeholders, and in response we launched a project to distribute certified reference materials (CRMs) to the users of our methods for the purpose of internal quality control. In addition to these activities, we have developed time- and cost-effective detection methods, such as a new screening method to simultaneously detect the sequence of Cauliflower mosaic virus 35S promoter (p35S) and the construct-specific sequence of GA21 event utilizing multiplex real-time polymerase chain reaction (PCR). We also developed a qualitative nonaplex PCR detection method, which allows the simultaneous detection of eight events of GM maize lines. Because the influx of any unapproved and unknown GMOs into the Japanese market is not permitted, we continue to explore this issue.

scholarly journals Wave-shaped microfluidic chip assisted point-of-care testing for accurate and rapid diagnosis of infections

2021 ◽  
Author(s):  
Binfeng Yin ◽  
Xinhua Wan ◽  
Mingzhu Yang ◽  
Changcheng Qian ◽  
A S M Muhtasim Fuad Sohan
Keyword(s):  
Microfluidic Chip ◽  
Limit Of Detection ◽  
Point Of Care ◽  
Simultaneous Detection ◽  
Accurate Diagnosis ◽  
Point Of Care Testing ◽  
Promising Alternative ◽  
Reactive Protein ◽  
Linear Relationships ◽  
Multiple Biomarkers

Abstract Background: Simultaneous and timely detection of C-reactive protein (CRP), procalcitonin (PCT), and interleukin-6 (IL-6) provides effective information for the accurate diagnosis of infections. Early diagnosis and classification of infections increase the cure rate while decreasing complications, which is significant for severe infections, especially for war surgery. However, traditional methods rely on laborious operations and bulky devices. On the other hand, point-of-care (POC) methods suffer from limited robustness and accuracy. Therefore, it is of urgent demand to develop POC devices for rapid and accurate diagnosis of infections to fulfill on-site militarized requirements.Methods: We developed a wave-shaped microfluidic chip (WMC) assisted multiplexed detection platform (WMC-MDP). WMC-MDP reduces detection time and improves repeatability through premixing of the samples and reaction of the reagents. We further combined the detection platform with the streptavidin-biotin (SA-B) amplified system to enhance the sensitivity while using chemiluminescence (CL) intensity as signal readout. We realized simultaneous detection of CRP, PCT, and IL-6 on the detection platform and evaluated the sensitivity, linear range, selectivity, and repeatability. Finally, we finished detecting 15 samples from volunteers and compared the results with commercial ELISA kits.Results: Detection of CRP, PCT, and IL-6 exhibited good linear relationships between CL intensities and concentrations in the range of 1.25-40 μg/mL, 0.4-12.8 ng/mL, and 50-1600 pg/mL. The limit of detection (LOD) of CRP, PCT, and IL-6 were 0.54 μg/mL, 0.11 ng/mL, and 16.25 pg/mL, respectively. WMC-MDP is capable of good adequate selectivity and repeatability. The whole detection procedure takes only 22 minutes that meets the requirements of a POC device. Results of 15 samples from volunteers were consistent with the results detected by commercial ELISA kits.Conclusion: WMC-MDP allows simultaneous, rapid, and sensitive detection of CRP, PCT, and IL-6 with satisfactory selectivity and repeatability, requiring minimal manipulation. However, WMC-MDP takes advantage of being a microfluidic device showing the coefficients of variation less than 10% enabling WMC-MDP to be a type of POCT. Therefore, WMC-MDP provides a promising alternative to point-of-care testing (POCT) of multiple biomarkers. We believe the practical application of WMC-MDP in militarized fields will revolutionize infection diagnosis for soldiers.

scholarly journals Simultaneous detection of multiple HPV DNA via bottom-well microfluidic chip within an infra-red PCR platform

2018 ◽  
Vol 12 (2) ◽  
pp. 024109 ◽  
Author(s):  
Wenjia Liu ◽  
Antony Warden ◽  
Jiahui Sun ◽  
Guangxia Shen ◽  
Xianting Ding
Keyword(s):  
Microfluidic Chip ◽  
Simultaneous Detection ◽  
Hpv Dna ◽  
Infra Red

scholarly journals Multifunctional microfluidic chip for cancer diagnosis and treatment

2021 ◽  
Vol 5 (1) ◽  
pp. 73-89
Author(s):  
Qiao-ru Guo ◽  
Ling-ling Zhang ◽  
Ji-fang Liu ◽  
Zhen Li ◽  
Jia-jun Li ◽  
...  
Keyword(s):  
Cancer Diagnosis ◽  
Microfluidic Chip ◽  
Diagnosis And Treatment

In situ multiplex detection of serum exosomal microRNAs using an all-in-one biosensor for breast cancer diagnosis

The Analyst ◽  
2020 ◽  
Vol 145 (9) ◽  
pp. 3289-3296 ◽  
Author(s):  
Huizhen Wang ◽  
Dinggeng He ◽  
Kejing Wan ◽  
Xiaowu Sheng ◽  
Hong Cheng ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Diagnosis ◽  
Simultaneous Detection ◽  
Breast Cancer Diagnosis ◽  
Multiplex Detection ◽  
Strand Displacement

Herein, a simple all-in-one biosensor based on a DNA three-way junction has been constructed for in situ simultaneous detection of multiple miRNAs by competitive strand displacement.

scholarly journals Simultaneous Detection Methods for Abused Diuretics in Urine and a Quantitative Determination Method for Trichlormethiazide in Urine

1984 ◽  
Vol 104 (10) ◽  
pp. 1101-1107 ◽  
Author(s):  
KATSUYOSHI UCHINO ◽  
YOSHIKAZU YAMAMURA ◽  
YUKIYA SAITOH ◽  
SADAO ISOZAKI ◽  
ZENZO TAMURA ◽  
...  
Keyword(s):  
Quantitative Determination ◽  
Simultaneous Detection ◽  
Detection Methods ◽  
Determination Method ◽  
Quantitative Determination Method

scholarly journals Printed Electrodes in Microfluidic Arrays for Cancer Biomarker Protein Detection

Biosensors ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 115
Author(s):  
Lasangi Dhanapala ◽  
Colleen E. Krause ◽  
Abby L. Jones ◽  
James F. Rusling
Keyword(s):  
Cancer Progression ◽  
Simultaneous Detection ◽  
Protein Detection ◽  
Medical Diagnostics ◽  
Cancer Diagnostics ◽  
Detection Methods ◽  
Enzyme Linked Immunosorbent Assay ◽  
Grand Challenge ◽  
Patient Records ◽  
Personalized Care

Medical diagnostics is trending towards a more personalized future approach in which multiple tests can be digitized into patient records. In cancer diagnostics, patients can be tested for individual protein and genomic biomarkers that detect cancers at very early stages and also be used to monitor cancer progression or remission during therapy. These data can then be incorporated into patient records that could be easily accessed on a cell phone by a health care professional or the patients themselves on demand. Data on protein biomarkers have a large potential to be measured in point-of-care devices, particularly diagnostic panels that could provide a continually updated, personalized record of a disease like cancer. Electrochemical immunoassays have been popular among protein detection methods due to their inherent high sensitivity and ease of coupling with screen-printed and inkjet-printed electrodes. Integrated chips featuring these kinds of electrodes can be built at low cost and designed for ease of automation. Enzyme-linked immunosorbent assay (ELISA) features are adopted in most of these ultrasensitive detection systems, with microfluidics allowing easy manipulation and good fluid dynamics to deliver reagents and detect the desired proteins. Several of these ultrasensitive systems have detected biomarker panels ranging from four to eight proteins, which in many cases when a specific cancer is suspected may be sufficient. However, a grand challenge lies in engineering microfluidic-printed electrode devices for the simultaneous detection of larger protein panels (e.g., 50–100) that could be used to test for many types of cancers, as well as other diseases for truly personalized care.

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