scholarly journals Simultaneous detection of multiple HPV DNA via bottom-well microfluidic chip within an infra-red PCR platform

2018 ◽  
Vol 12 (2) ◽  
pp. 024109 ◽  
Author(s):  
Wenjia Liu ◽  
Antony Warden ◽  
Jiahui Sun ◽  
Guangxia Shen ◽  
Xianting Ding
Keyword(s):  
Microfluidic Chip ◽  
Simultaneous Detection ◽  
Hpv Dna ◽  
Infra Red

scholarly journals Wave-shaped microfluidic chip assisted point-of-care testing for accurate and rapid diagnosis of infections

2021 ◽  
Author(s):  
Binfeng Yin ◽  
Xinhua Wan ◽  
Mingzhu Yang ◽  
Changcheng Qian ◽  
A S M Muhtasim Fuad Sohan
Keyword(s):  
Microfluidic Chip ◽  
Limit Of Detection ◽  
Point Of Care ◽  
Simultaneous Detection ◽  
Accurate Diagnosis ◽  
Point Of Care Testing ◽  
Promising Alternative ◽  
Reactive Protein ◽  
Linear Relationships ◽  
Multiple Biomarkers

Abstract Background: Simultaneous and timely detection of C-reactive protein (CRP), procalcitonin (PCT), and interleukin-6 (IL-6) provides effective information for the accurate diagnosis of infections. Early diagnosis and classification of infections increase the cure rate while decreasing complications, which is significant for severe infections, especially for war surgery. However, traditional methods rely on laborious operations and bulky devices. On the other hand, point-of-care (POC) methods suffer from limited robustness and accuracy. Therefore, it is of urgent demand to develop POC devices for rapid and accurate diagnosis of infections to fulfill on-site militarized requirements.Methods: We developed a wave-shaped microfluidic chip (WMC) assisted multiplexed detection platform (WMC-MDP). WMC-MDP reduces detection time and improves repeatability through premixing of the samples and reaction of the reagents. We further combined the detection platform with the streptavidin-biotin (SA-B) amplified system to enhance the sensitivity while using chemiluminescence (CL) intensity as signal readout. We realized simultaneous detection of CRP, PCT, and IL-6 on the detection platform and evaluated the sensitivity, linear range, selectivity, and repeatability. Finally, we finished detecting 15 samples from volunteers and compared the results with commercial ELISA kits.Results: Detection of CRP, PCT, and IL-6 exhibited good linear relationships between CL intensities and concentrations in the range of 1.25-40 μg/mL, 0.4-12.8 ng/mL, and 50-1600 pg/mL. The limit of detection (LOD) of CRP, PCT, and IL-6 were 0.54 μg/mL, 0.11 ng/mL, and 16.25 pg/mL, respectively. WMC-MDP is capable of good adequate selectivity and repeatability. The whole detection procedure takes only 22 minutes that meets the requirements of a POC device. Results of 15 samples from volunteers were consistent with the results detected by commercial ELISA kits.Conclusion: WMC-MDP allows simultaneous, rapid, and sensitive detection of CRP, PCT, and IL-6 with satisfactory selectivity and repeatability, requiring minimal manipulation. However, WMC-MDP takes advantage of being a microfluidic device showing the coefficients of variation less than 10% enabling WMC-MDP to be a type of POCT. Therefore, WMC-MDP provides a promising alternative to point-of-care testing (POCT) of multiple biomarkers. We believe the practical application of WMC-MDP in militarized fields will revolutionize infection diagnosis for soldiers.

A portable microfluidic chip system for cancer diagnosis with simultaneous detection methods

2012 ◽  
Author(s):  
Hyoungseon Choi ◽  
Kwang Bok Kim ◽  
Changsu Jun ◽  
Taek Dong Chung ◽  
Hee Chan Kim
Keyword(s):  
Cancer Diagnosis ◽  
Microfluidic Chip ◽  
Simultaneous Detection ◽  
Detection Methods

A Microfluidic Chip Integrated with a Flexible CNT-PDMS Electrode Array for Simultaneous Detection of Cd 2+, Pb 2+, Hg 2+

2021 ◽  
Author(s):  
Wenhong Zhang ◽  
Zhipeng Xu ◽  
Li Wang ◽  
Weiguang Su ◽  
Xiangxiang Zhou ◽  
...  
Keyword(s):  
Microfluidic Chip ◽  
Simultaneous Detection ◽  
Electrode Array

Microfluidic chip coupled with optical biosensors for simultaneous detection of multiple analytes: A review

2019 ◽  
Vol 126 ◽  
pp. 697-706 ◽  
Author(s):  
Zerong Liao ◽  
Yang Zhang ◽  
Yongrui Li ◽  
Yunfei Miao ◽  
Shimeng Gao ◽  
...  
Keyword(s):  
Microfluidic Chip ◽  
Simultaneous Detection ◽  
Optical Biosensors ◽  
Multiple Analytes

A real-time LAMP-based dual-sample microfluidic chip for rapid and simultaneous detection of multiple waterborne pathogenic bacteria from coastal waters

2021 ◽  
Author(s):  
Jinglei Jin ◽  
Lijun Duan ◽  
Jiali Fu ◽  
Fangchao Chai ◽  
Qianjin Zhou ◽  
...  
Keyword(s):  
Real Time ◽  
Microfluidic Chip ◽  
Coastal Waters ◽  
Pathogenic Bacteria ◽  
Simultaneous Detection ◽  
Lamp Method

(1) A dual-sample microfluidic chip integrated LAMP method was developed to simultaneously detect 10 waterborne pathogenic bacteria within 35 min. (2) Its operations are in a highly automated format and it is suitable for on-site detection.

scholarly journals Persistence of Human Papillomavirus DNA in Cervical Lesions After Treatment With Diathermic Large Loop Excision

1998 ◽  
Vol 6 (5) ◽  
pp. 214-219 ◽  
Author(s):  
A. L. Distéfano ◽  
M. A. Picconi ◽  
L. V. Alonio ◽  
D. Dalbert ◽  
J. Mural ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Blot Hybridization ◽  
Simultaneous Detection ◽  
Cervical Lesions ◽  
Dot Blot ◽  
Hpv Dna ◽  
Viral Type ◽  
Large Loop ◽  
Single Stranded Conformational Polymorphism

Objective:The aim of this study was to identify human papillomavirus (HPV) in cervical intraepithelial neoplasia (CIN) lesions and to evaluate the persistence of viral DNA after diathermic large loop excision (DLLE) treatment.Study Design:Biopsies from 36 patients with low- and high-grade CIN lesions were studied before and after DLLE treatment looking for HPV sequences. DNA was extracted to perform a radioactive polymerase chain reaction (PCR) using GP 5,6 generic primers. PCR products were analyzed by the single-stranded conformational polymorphism (SSCP) which is a simultaneous detection and typing method. Dot-blot hybridization with generic and type-specific biotinylated oligonucleotide probes was applied in some cases.Results:HPV DNA was found in all pretreatment samples, and the viral type was identified in 80% of them, HPV 16 being the most prevalent. The viral type coincided with that detected in the first biopsy in all except one case. Seventy five percent of the patients (27 cases) were negative for CIN at follow up, but 50% of them remained HPV DNA positive.Conclusion:DLLE treatment was effective in removing the CIN lesion but not the HPV. This fact points out the need to asses the presence of HPV in DNA during the follow-up, since viral persistence has been considered a high risk factor for recurrence and/or malignant transformation.

scholarly journals A decoupler-free simple paper microchip capillary electrophoresis device for simultaneous detection of dopamine, epinephrine and serotonin

RSC Advances ◽  
2020 ◽  
Vol 10 (43) ◽  
pp. 25487-25495 ◽  
Author(s):  
Appan Roychoudhury ◽  
Kevin Antony Francis ◽  
Jay Patel ◽  
Sandeep Kumar Jha ◽  
Suddhasatwa Basu
Keyword(s):  
Capillary Electrophoresis ◽  
Microfluidic Chip ◽  
Simultaneous Detection ◽  
Amperometric Detection ◽  
Microchip Capillary Electrophoresis ◽  
Paper Microfluidic

This paper demonstrates a simplified configuration for capillary electrophoresis-amperometric detection using paper microfluidic chip for separation and simultaneous detection of three clinically relevant neurochemicals without using any decouplers.

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