Highly Efficient Doubled‐Haploid Production in Wheat ( Triticum aestivum L.) via Induced Microspore Embryogenesis

Crop Science ◽  
2002 ◽  
Vol 42 (3) ◽  
pp. 686-692 ◽  
Author(s):  
Weiguo Liu ◽  
Ming Y. Zheng ◽  
Enrique A. Polle ◽  
Calvin F. Konzak
Keyword(s):  
Triticum Aestivum ◽  
Doubled Haploid ◽  
Microspore Embryogenesis ◽  
Triticum Aestivum L ◽  
Highly Efficient ◽  
Haploid Production

scholarly journals In vitro anther culture as efficiently applied technique for doubled haploid production of wheat (Triticum aestivum L.)

2021 ◽  
Vol 58 (1) ◽  
pp. 31-45
Author(s):  
Osama Kanbar ◽  
Csaba Lantos ◽  
Janos Pauk
Keyword(s):  
Triticum Aestivum ◽  
Anther Culture ◽  
Doubled Haploid ◽  
Chromosome Doubling ◽  
Culture Media ◽  
Triticum Aestivum L ◽  
Technology Application ◽  
Breeding Material ◽  
Haploid Production

In vitro anther culture is the most commonly-used technology for doubled haploid production in wheat (Triticum aestivum L.) and various cereals. The technology application allows the genetic attainment of homozygous pure lines from heterozygous breeding material in a single generation, and has become a rapid alternative to the conventional breeding methods. This technology also contributes to more accurately assess QTL × environmental interactions and has been applied in: genetic researches for marker-trait association study, genomics and as a target for transformation, genetic engineering, gene mapping, and mapping of quantitative trait loci (QTLs). This paper reviews the most important factors that limit the efficiency of winter wheat (Triticum aestivum L.) anther culture, such as genotype dependency and albinism, in addition to other affecting factors including the collection time of tillers and physiological growth environments of plants. Factors that improve the efficiency of wheat anther culture in mitigating both genotypic dependency and albinism have been included in this review, too, for example, genetic improvements by following a strategy to realize this purpose through using responsive breeding material for crossing, application of convenient stress pre-treatments (cold pre-treatments, heat shock treatment, colchicine, hormones, and chemicals) to induce androgenesis, and improvement of the composition of anther culture media and culture conditions. We have also highlighted the methods of chromosome doubling for the haploid wheat produced by anther culture androgenesis, such as spontaneous chromosome doubling or use of colchicine and anti-mitotic chemicals at an early stage of anther culture or as root immersion treatment.

Highly Efficient Doubled-Haploid Production in Wheat ( L.) via Induced Microspore Embryogenesis

Crop Science ◽  
2002 ◽  
Vol 42 (3) ◽  
pp. 686 ◽  
Author(s):  
Weiguo Liu ◽  
Ming Y. Zheng ◽  
Enrique A. Polle ◽  
Calvin F. Konzak
Keyword(s):  
Doubled Haploid ◽  
Microspore Embryogenesis ◽  
Highly Efficient ◽  
Haploid Production

Haploid production of Australian wheat (Triticum aestivum L.) cultivars through wheat × maize (Zea mays L.) crosses

1997 ◽  
Vol 48 (8) ◽  
pp. 1207 ◽  
Author(s):  
K. Suenaga ◽  
A. R. Morshedi ◽  
N. L. Darvey
Keyword(s):  
Triticum Aestivum ◽  
Zea Mays ◽  
Zea Mays L ◽  
Culture Method ◽  
Triticum Aestivum L ◽  
Wheat Cultivars ◽  
Culture Methods ◽  
Haploid Production ◽  
Australian Wheat ◽  
Haploid Embryos

In order to reduce the labour for wheat haploid production through wheat maize crosses, several emasculation methods were investigated in combination with the ‘spike culture method’. Although the standard method whereby wheat spikes were hand-emasculated and pollinated on the day of anthesis gave a higher efficiency, the ‘non-emasculation method’ gave a comparable response. The use of the non-emasculation method and spike culture could eliminate much of the labour required for emasculation and treatment with 2,4-D, which is normally applied by injection into wheat internodes or by dropping onto florets after pollination with maize. Most of the selfed seeds were easily identified by the presence of endosperm, and the probability of contamination by the embryos originating from selfing among the presumptive haploid embryos in the non-emasculation method was very low. Twenty-seven Australian wheat cultivars were investigated for haploid production through wheat × maize crosses using the non-emasculation and spike culture methods. All of the 27 cultivars produced embryos after crossing with maize, with a mean efficiency of 33·1% (embryos/florets). Except for one cultivar, Tincurrin, plants were recovered from all of the cultivars (average of 61·5% including Tinccurin). This innovation of haploid production through wheat maize crosses is discussed.

Cytogenetic studies of progenies from crosses between 'Centurk' wheat and its doubled haploids derived from anther culture

Genome ◽  
1989 ◽  
Vol 32 (4) ◽  
pp. 622-628 ◽  
Author(s):  
Sawsan S. Youssef ◽  
R. Morris ◽  
P. S. Baenziger ◽  
C. M. Papa
Keyword(s):  
Triticum Aestivum ◽  
Chromosome Pairing ◽  
Doubled Haploid ◽  
Structural Changes ◽  
Meiotic Chromosome ◽  
Doubled Haploids ◽  
Triticum Aestivum L ◽  
Root Tips ◽  
Doubled Haploid Lines ◽  
A Minor

Karyotype stability, which is essential when using wheat (Triticum aestivum L.) doubled haploids in a breeding program, was evaluated in 14 anther-derived doubled-haploid lines after at least three generations of selfing, by crossing them as females with the parent cultivar 'Centurk' and doing cytological studies on the progenies. There were no deviations from the hexaploid chromosome number (2n = 42) in root tips. Meiotic chromosome pairing was as stable as that in the control ('Centurk' × 'Centurk') in most progenies. Chromosomal structural changes and (or) behavioral deviations were detected at the metaphase I, anaphase I, telophase I, and quartet stages of meiosis in a minor proportion of the cells. The frequencies of multivalents, lagging bivalents and univalents, bridges, and micronuclei were higher in some progenies than in the control. Chromosomal fragments were infrequent. The ranges in percentages of normal cells were 72.4–90.0 at anaphase I, 76.4–92.6 at telophase I, and 82.6–93.2 at quartet stages in the doubled-haploid progenies, compared with 95–100, 92–100, and 94–96, respectively, in the control. On the basis of these results, the doubled-haploid lines should produce enough normal gametes to provide adequate seed supplies when they are used as parents in wheat cultivar and population improvement.Key words: Triticum aestivum, chromosome pairing, chromosome aberrations, gametoclonal variation.

Stettler hard red spring wheat

2009 ◽  
Vol 89 (5) ◽  
pp. 945-951 ◽  
Author(s):  
R M DePauw ◽  
R E Knox ◽  
F R Clarke ◽  
J M Clarke ◽  
T N McCaig
Keyword(s):  
Triticum Aestivum ◽  
Grain Yield ◽  
Spring Wheat ◽  
Doubled Haploid ◽  
Triticum Aestivum L ◽  
Kernel Weight ◽  
Hard Red Spring Wheat ◽  
Volume Weight ◽  
Grain Volume Weight ◽  
End Use

Based on 34 replicated trials over 3 yr, Stettler, a doubled haploid hard red spring wheat (Triticum aestivum L.), expressed significantly higher grain yield than all checks except Superb. Wheat and flour protein concentration were significantly greater than all of the checks except Lillian. It matured significantly later than AC Barrie and Katepwa but earlier than Superb. Stettler was significantly shorter than all of the checks except Superb and was more resistant to lodging than Katepwa and Laura. Stettler had high grain volume weight and intermediate kernel weight relative to the checks, and meets the end-use quality specifications of the Canada Western Red Spring wheat market class. Stettler expressed resistance to prevalent races of stem rust, common bunt and loose smut, with moderate susceptibility to prevalent races of leaf rust and fusarium head blight.Key words: Triticum aestivum L., cultivar description, grain yield, protein, disease resistance, doubled haploid

scholarly journals Generation of doubled haploid lines from winter wheat (Triticum aestivum L.) breeding material using in vitro anther culture

2020 ◽  
Vol 56 (No. 4) ◽  
pp. 150-158
Author(s):  
Osama Zuhair Kanbar ◽  
Csaba Lantos ◽  
Paul Karumba Chege ◽  
Erzsébet Kiss ◽  
János Pauk
Keyword(s):  
Triticum Aestivum ◽  
Winter Wheat ◽  
Anther Culture ◽  
Doubled Haploid ◽  
Genetic Material ◽  
Triticum Aestivum L ◽  
Average Value ◽  
Doubled Haploid Lines ◽  
Dh Lines

We investigated the anther culture (AC) efficiency of thirteen F<sub>4</sub> combinations of winter wheat (Triticum aestivum L.). The genotype dependency was assessed during the induction of the androgenic entities, i.e. embryo-like structures (ELS), regenerated-, green-, albino-, and transplanted plantlets. The number of green plantlets per 100 anthers (GP/100A) varied from 0.36 to 24.74 GP/100A with a mean of 8.31 GP/100A. Albino plantlets (AP) occurred in each combination, ranging from 0.20 to 22.80 AP/100A with an average value of 5.59 AP/100A. Between 25–87.76 doubled haploid (DH) plants per 100 acclimatised plantlets (DH/100ADP), depending on the combination, with a mean of 59.74% were recovered. We have found the highest DH production in the combinations Béres/Midas, Kalász/Tacitus, Béres/Pamier, and Premio/5009. This improves remarkably the choice of basic genetic material in subsequent crossing programmes. These observations emphasise the usability and efficiency of in vitro AC in producing a large number of DH lines for breeding and the applied researches of winter wheat. Although albinism was found in each combination, it was mitigated by the in vitro AC application.

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