Highly Efficient Doubled-Haploid Production in Wheat ( L.) via Induced Microspore Embryogenesis

Crop Science ◽  
2002 ◽  
Vol 42 (3) ◽  
pp. 686 ◽  
Author(s):  
Weiguo Liu ◽  
Ming Y. Zheng ◽  
Enrique A. Polle ◽  
Calvin F. Konzak
Keyword(s):  
Doubled Haploid ◽  
Microspore Embryogenesis ◽  
Highly Efficient ◽  
Haploid Production

Optimization of chromosome doubling treatment for efficient in vivo doubled haploid production in maize

2021 ◽  
pp. 1-10
Author(s):  
Sourbh Kumar ◽  
Uttam Chandel ◽  
Satish Kumar Guleria
Keyword(s):  
Doubled Haploid ◽  
Seed Set ◽  
Chromosome Doubling ◽  
Pollen Viability ◽  
Haploid Inducer ◽  
Haploid Production ◽  
Maize Genotypes ◽  
Different Doses

Abstract An investigation to optimize the protocol for application of colchicine for enhancing the doubled haploid production in maize was done. 106 maize genotypes were used as maternal parents, whereas, pollen source involved tropically adopted haploid inducer (TAIL P1 and TAIL hybrid). After the elimination of chromosomes of inducer lines, haploid seeds were obtained from the crosses. Haploid seedlings were treated with three different doses, such as 0.04, 0.06 and 0.08 per cent of colchicines for different durations (8, 12 and 15 hours). The response of various colchicine concentrations applied for different time durations revealed significant differences at P ≤ 0.05 for various parameters viz., per cent plants survivability, stalk colour, the fertility of tassel, silk present/absent, pollen viability, seed set and per cent doubled haploid formation. In maize, colchicine doses of 0.04 per cent for 12 hours and 0.06 per cent for 8 hours, respectively were established as optimum for enhanced doubled haploid production. But among these two, 0.04 per cent for 12 hours was observed to be best dose for doubled haploid production in maize.

Maternal doubled haploid production in interploidy hybridization between Brassica napus and Brassica allooctaploids

Planta ◽  
2017 ◽  
Vol 247 (1) ◽  
pp. 113-125 ◽  
Author(s):  
Shaohong Fu ◽  
Liqin Yin ◽  
Mingchao Xu ◽  
Yun Li ◽  
Maolin Wang ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Doubled Haploid ◽  
Haploid Production

scholarly journals Suppression of Metacaspase- and Autophagy-Dependent Cell Death Improves Stress-Induced Microspore Embryogenesis in Brassica napus

2020 ◽  
Author(s):  
Eduardo Berenguer ◽  
Elena A Minina ◽  
Elena Carneros ◽  
Ivett Bárány ◽  
Peter V Bozhkov ◽  
...  
Keyword(s):  
Cell Death ◽  
Brassica Napus ◽  
Embryonic Development ◽  
Doubled Haploid ◽  
Microspore Embryogenesis ◽  
Brassica Species ◽  
Cysteine Proteases ◽  
Plant Production ◽  
Haploid Plant

Abstract Microspore embryogenesis is a biotechnological process that allows us to rapidly obtain doubled-haploid plants for breeding programs. The process is initiated by the application of stress treatment, which reprograms microspores to embark on embryonic development. Typically, a part of the microspores undergoes cell death that reduces the efficiency of the process. Metacaspases (MCAs), a phylogenetically broad group of cysteine proteases, and autophagy, the major catabolic process in eukaryotes, are critical regulators of the balance between cell death and survival in various organisms. In this study, we analyzed the role of MCAs and autophagy in cell death during stress-induced microspore embryogenesis in Brassica napus. We demonstrate that this cell death is accompanied by the transcriptional upregulation of three BnMCA genes (BnMCA-Ia, BnMCA-IIa and BnMCA-IIi), an increase in MCA proteolytic activity and the activation of autophagy. Accordingly, inhibition of autophagy and MCA activity, either individually or in combination, suppressed cell death and increased the number of proembryos, indicating that both components play a pro-cell death role and account for decreased efficiency of early embryonic development. Therefore, MCAs and/or autophagy can be used as new biotechnological targets to improve in vitro embryogenesis in Brassica species and doubled-haploid plant production in crop breeding and propagation programs.

Early diagnosis of ploidy status in doubled haploid production of maize by stomata length and flow cytometry measurements

2019 ◽  
Vol 138 (3) ◽  
pp. 266-276 ◽  
Author(s):  
Willem S. Molenaar ◽  
Evellyn Giselly Oliveira Couto ◽  
Hans‐Peter Piepho ◽  
Albrecht E. Melchinger
Keyword(s):  
Flow Cytometry ◽  
Early Diagnosis ◽  
Doubled Haploid ◽  
Haploid Production ◽  
Ploidy Status
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