Modification of important factors for efficient microspore embryogenesis and doubled haploid production in field grown white cabbage (Brassica oleracea var. capitata L.) genotypes in India

2018 ◽  
Vol 233 ◽  
pp. 178-187 ◽  
Author(s):  
Reeta Bhatia ◽  
S.S. Dey ◽  
Chander Parkash ◽  
Kanika Sharma ◽  
Shritika Sood ◽  
...  
Keyword(s):  
Brassica Oleracea ◽  
Doubled Haploid ◽  
Microspore Embryogenesis ◽  
Haploid Production ◽  
White Cabbage

scholarly journals The Influence of pH on Microspore Embryogenesis of White Cabbage (Brassica oleracea L.)

2013 ◽  
Vol 5 (4) ◽  
pp. 485-489 ◽  
Author(s):  
Tina Oana CRISTEA
Keyword(s):  
Brassica Oleracea ◽  
Microspore Culture ◽  
Microspore Embryogenesis ◽  
Critical Factor ◽  
Cellular Level ◽  
Strong Base ◽  
White Cabbage ◽  
In Vitro Microspore Culture ◽  
Influence Of Ph

In vitro microspore culture is one of the top techniques utilised now-a-days for the obtaining of double haploid plants in many plant species, including Brassica. The pH of the medium is a critical factor for the success of In vitro microspore culture as it influences the invertase enzyme activity, translated at cellular level through an acceleration or reduction of sucrose cleavage. The results published until now shows rather contradictory findings, as the response of microspores have been proved to be highly depending on genotypes, most of them being focused on Brassica napus. Thus, in the present study, the effect of different NLN liquid medium pH, ranging between 5.0 to 7.0 were tested in order to establish the most suitable pH for the expression of embryogenic competences of microspores cultivated on medium In vitro and ultimately for the obtaining of microspore-derived embryos. Among the 11 values of pH tested, the best results were obtained on variants with pH 5.8 and 6.0, both in what concern the maintaining of microspores viability and the number of microspore-derived embryos. The findings of the present study provide a strong base for the establishment of an efficient protocol for the In vitro culture of microspore at Brassica oleracea L. genotypes with Romanian origin.

Efficient doubled haploid production in microspore culture of loose-curd cauliflower (Brassica oleracea var. botrytis)

Euphytica ◽  
2013 ◽  
Vol 195 (3) ◽  
pp. 467-475 ◽  
Author(s):  
Honghui Gu ◽  
Zhengqing Zhao ◽  
Xiaoguang Sheng ◽  
Huifang Yu ◽  
Jiansheng Wang
Keyword(s):  
Brassica Oleracea ◽  
Doubled Haploid ◽  
Microspore Culture ◽  
Haploid Production

scholarly journals Improvements of doubled haploid production protocol for white cabbage (Brassica oleracea var. capitata L.)

2018 ◽  
Vol 30 (1) ◽  
pp. 57-66 ◽  
Author(s):  
Katarina Rudolf Pilih ◽  
Urška Karolina Potokar ◽  
Borut Bohanec
Keyword(s):  
Brassica Oleracea ◽  
Doubled Haploid ◽  
Microspore Culture ◽  
Petri Dish ◽  
Abnormal Development ◽  
Haploid Induction ◽  
Embryo Formation ◽  
White Cabbage ◽  
Dh Lines ◽  
Further Development

Abstract Protocols leading to the development of doubled haploid (DH) lines by microspore culture are widely used in white cabbage (Brassica oleracea var. capitata L.), but efficiency varies according to the cultivar and induction procedure. Forty different genotypes consisting of F1 cultivars and their crosses with responsive doubled haploid lines were tested to evaluate the androgenic response. In total, 20,032 embryos were produced. On average, the haploid induction response of F1 cultivars was 7.0 embryos/Petri dish, but the average of these hybrids crossed to responsive DH lines was 26.6 embryos/Petri dish. In seven reciprocal crosses, a difference was observed in just one, meaning that the maternal effect probably has a minor influence on haploid embryogenesis in cabbage. Addition of 0.02% activated charcoal (AC) to the induction media increased embryo formation in several low-responsive genotypes, but its effect on embryo formation of high-responsive genotypes was predominantly negative, although larger embryos were formed on media containing AC than without AC. Further development into plantlets was tested by two procedures. Formed embryos were either transferred directly to regeneration medium or treated with abscisic acid and desiccated for 4 weeks. Regrowth and further development reached on average 15.5 and 57.6%, for the first and second procedures, respectively. Plantlets developed by direct transfer often exhibited abnormal development or hyperhydricity, unlike the desiccated embryos. Spontaneous diploidisation of embryos reached 42.5% in total and was not affected by AC added to the induction media.

Highly Efficient Doubled-Haploid Production in Wheat ( L.) via Induced Microspore Embryogenesis

Crop Science ◽  
2002 ◽  
Vol 42 (3) ◽  
pp. 686 ◽  
Author(s):  
Weiguo Liu ◽  
Ming Y. Zheng ◽  
Enrique A. Polle ◽  
Calvin F. Konzak
Keyword(s):  
Doubled Haploid ◽  
Microspore Embryogenesis ◽  
Highly Efficient ◽  
Haploid Production

Genotypic differences in nitrogen efficiency of white cabbage (Brassica oleracea L.)

2009 ◽  
Vol 328 (1-2) ◽  
pp. 313-325 ◽  
Author(s):  
Gunda Schulte auf’m Erley ◽  
Elsa Rakhmi Dewi ◽  
Olani Nikus ◽  
Walter J. Horst
Keyword(s):  
Brassica Oleracea ◽  
Nitrogen Efficiency ◽  
Genotypic Differences ◽  
White Cabbage ◽  
Brassica Oleracea L

Optimization of chromosome doubling treatment for efficient in vivo doubled haploid production in maize

2021 ◽  
pp. 1-10
Author(s):  
Sourbh Kumar ◽  
Uttam Chandel ◽  
Satish Kumar Guleria
Keyword(s):  
Doubled Haploid ◽  
Seed Set ◽  
Chromosome Doubling ◽  
Pollen Viability ◽  
Haploid Inducer ◽  
Haploid Production ◽  
Maize Genotypes ◽  
Different Doses

Abstract An investigation to optimize the protocol for application of colchicine for enhancing the doubled haploid production in maize was done. 106 maize genotypes were used as maternal parents, whereas, pollen source involved tropically adopted haploid inducer (TAIL P1 and TAIL hybrid). After the elimination of chromosomes of inducer lines, haploid seeds were obtained from the crosses. Haploid seedlings were treated with three different doses, such as 0.04, 0.06 and 0.08 per cent of colchicines for different durations (8, 12 and 15 hours). The response of various colchicine concentrations applied for different time durations revealed significant differences at P ≤ 0.05 for various parameters viz., per cent plants survivability, stalk colour, the fertility of tassel, silk present/absent, pollen viability, seed set and per cent doubled haploid formation. In maize, colchicine doses of 0.04 per cent for 12 hours and 0.06 per cent for 8 hours, respectively were established as optimum for enhanced doubled haploid production. But among these two, 0.04 per cent for 12 hours was observed to be best dose for doubled haploid production in maize.

Maternal doubled haploid production in interploidy hybridization between Brassica napus and Brassica allooctaploids

Planta ◽  
2017 ◽  
Vol 247 (1) ◽  
pp. 113-125 ◽  
Author(s):  
Shaohong Fu ◽  
Liqin Yin ◽  
Mingchao Xu ◽  
Yun Li ◽  
Maolin Wang ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Doubled Haploid ◽  
Haploid Production
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