scholarly journals CHARACTERIZATION OF AN IMMORTALIZED HEPATIC STELLATE CELL LINE ESTABLISHED FROM METALLOTHIONEIN-NULL MICE

2006 ◽  
Vol 31 (4) ◽  
pp. 391-398 ◽  
Author(s):  
Nobuhiko MIURA ◽  
Yoshitaka KANAYAMA ◽  
Wakako NAGAI ◽  
Tatsuya HASEGAWA ◽  
Yoshiyuki SEKO ◽  
...  
Keyword(s):  
Cell Line ◽  
Hepatic Stellate Cell ◽  
Stellate Cell

scholarly journals Proteomic analysis of extracellular matrix from the hepatic stellate cell line LX-2 identifies CYR61 and Wnt-5a as novel constituents of fibrotic liver

2012 ◽  
Vol 11 (8) ◽  
pp. 4052-4064 ◽  
Author(s):  
S. Tamir Rashid ◽  
Jonathan D. Humphries ◽  
Adam Byron ◽  
Ameet Dhar ◽  
Janet A. Askari ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Cell Line ◽  
Hepatic Stellate Cell ◽  
Proteomic Analysis ◽  
Stellate Cell ◽  
Fibrotic Liver

Activity and expression of ecto-5′-nucleotidase/CD73 are increased during phenotype conversion of a hepatic stellate cell line

Life Sciences ◽  
2008 ◽  
Vol 82 (1-2) ◽  
pp. 21-29 ◽  
Author(s):  
Cláudia M.B. Andrade ◽  
Gislaine C. Roesch ◽  
Márcia R. Wink ◽  
Eduardo L.M. Guimarães ◽  
Luiz F. Souza ◽  
...  
Keyword(s):  
Cell Line ◽  
Hepatic Stellate Cell ◽  
Stellate Cell

Establishment of an Immortalized Human Hepatic Stellate Cell Line to Develop Antifibrotic Therapies

2003 ◽  
Vol 12 (5) ◽  
pp. 499-507 ◽  
Author(s):  
Norikuni Shibata ◽  
Takamasa Watanabe ◽  
Teru Okitsu ◽  
Masakiyo Sakaguchi ◽  
Michihiko Takesue ◽  
...  
Keyword(s):  
Cell Line ◽  
Hepatic Stellate Cell ◽  
Type I Collagen ◽  
Replicative Senescence ◽  
Stellate Cell ◽  
Smooth Muscle Actin ◽  
Population Doubling ◽  
Type I ◽  
Human Hepatic Stellate Cell ◽  
Ifn Γ

Because human hepatic stellate cells (HSCs) perform a crucial role in the progress of hepatic fibrosis, it is of great value to establish an immortalized human cell line that exhibits HSC characteristics and grows well in tissue cultures for the development of antifibrotic therapies. Thus, we engineered an immortalized human hepatic stellate cell (HSC) line TWNT-4 by retrovirally inducing human telomerase reverse transcriptase (hTERT) into LI 90 cells established from a human liver mesenchymal tumor. Parental LI 90 entered replicative senescence, whereas TWNT-4 showed telomerase activity and proliferated for more than population doubling level (PDL) 200 without any crisis. TWNT-4 expressed platelet-derived growth factor-β receptor (PDGF-βR), α-smooth muscle actin (α-SMA), and type I collagen (α1) and was considered to be an activated form of HSCs. Treatment of TWNT-4 cells with either 100 U/ml of IFN-γ or 1 ng/ml of rapamycin (Rapa) for 14 days led to lower expression of type I collagen (α1) at RNA and protein levels. Exposure of TWNT-4 cells to both of IFN-γ (10 U/ml) and Rapa (0.1 ng/ml) for 14 days effectively decreased the expression of type I collagen (α1), PDGF-βR, and α-SMA expression and suppressed TGF-β1 secretion of TWNT-4 cells. We successfully induced apoptosis by transducing TNF-related apoptosis-inducing ligand (TRAIL) into TWNT-4 cells using adenovirus vectors Ad/GT-TRAIL and Ad/PGK-GV-17. These findings suggested that immortalized activated HSC line TWNT-4 would be a useful means to develop antifibrotic therapies.

Relationship between oxidative stress levels and activation state on a hepatic stellate cell line

2006 ◽  
Vol 26 (4) ◽  
pp. 477-485 ◽  
Author(s):  
E. L. M. Guimaraes ◽  
M. F. S. Franceschi ◽  
I. Grivicich ◽  
F. Dal-pizzol ◽  
J. C. F. Moreira ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cell Line ◽  
Hepatic Stellate Cell ◽  
Stellate Cell ◽  
Stress Levels

PAV-1, a new rat hepatic stellate cell line converts retinol into retinoic acid, a process altered by ethanol

2002 ◽  
Vol 34 (8) ◽  
pp. 1017-1029 ◽  
Author(s):  
Patrick Sauvant ◽  
Vincent Sapin ◽  
Armand Abergel ◽  
Carsten K Schmidt ◽  
Loı̈c Blanchon ◽  
...  
Keyword(s):  
Retinoic Acid ◽  
Cell Line ◽  
Hepatic Stellate Cell ◽  
Stellate Cell
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