scholarly journals Establishment and Characterization of an Immortalized Human Hepatic Stellate Cell Line for Applications in Co-Culturing with Immortalized Human Hepatocytes

2015 ◽  
Vol 12 (3) ◽  
pp. 248-255 ◽  
Author(s):  
XiaoPing Pan ◽  
Yini Wang ◽  
XiaoPeng Yu ◽  
JianZhou Li ◽  
Ning Zhou ◽  
...  
Keyword(s):  
Cell Line ◽  
Hepatic Stellate Cell ◽  
Stellate Cell ◽  
Human Hepatocytes ◽  
Human Hepatic Stellate Cell

Establishment of an Immortalized Human Hepatic Stellate Cell Line to Develop Antifibrotic Therapies

2003 ◽  
Vol 12 (5) ◽  
pp. 499-507 ◽  
Author(s):  
Norikuni Shibata ◽  
Takamasa Watanabe ◽  
Teru Okitsu ◽  
Masakiyo Sakaguchi ◽  
Michihiko Takesue ◽  
...  
Keyword(s):  
Cell Line ◽  
Hepatic Stellate Cell ◽  
Type I Collagen ◽  
Replicative Senescence ◽  
Stellate Cell ◽  
Smooth Muscle Actin ◽  
Population Doubling ◽  
Type I ◽  
Human Hepatic Stellate Cell ◽  
Ifn Γ

Because human hepatic stellate cells (HSCs) perform a crucial role in the progress of hepatic fibrosis, it is of great value to establish an immortalized human cell line that exhibits HSC characteristics and grows well in tissue cultures for the development of antifibrotic therapies. Thus, we engineered an immortalized human hepatic stellate cell (HSC) line TWNT-4 by retrovirally inducing human telomerase reverse transcriptase (hTERT) into LI 90 cells established from a human liver mesenchymal tumor. Parental LI 90 entered replicative senescence, whereas TWNT-4 showed telomerase activity and proliferated for more than population doubling level (PDL) 200 without any crisis. TWNT-4 expressed platelet-derived growth factor-β receptor (PDGF-βR), α-smooth muscle actin (α-SMA), and type I collagen (α1) and was considered to be an activated form of HSCs. Treatment of TWNT-4 cells with either 100 U/ml of IFN-γ or 1 ng/ml of rapamycin (Rapa) for 14 days led to lower expression of type I collagen (α1) at RNA and protein levels. Exposure of TWNT-4 cells to both of IFN-γ (10 U/ml) and Rapa (0.1 ng/ml) for 14 days effectively decreased the expression of type I collagen (α1), PDGF-βR, and α-SMA expression and suppressed TGF-β1 secretion of TWNT-4 cells. We successfully induced apoptosis by transducing TNF-related apoptosis-inducing ligand (TRAIL) into TWNT-4 cells using adenovirus vectors Ad/GT-TRAIL and Ad/PGK-GV-17. These findings suggested that immortalized activated HSC line TWNT-4 would be a useful means to develop antifibrotic therapies.

Human hepatic stellate cell line (LX-2) exhibits characteristics of bone marrow-derived mesenchymal stem cells

2011 ◽  
Vol 91 (3) ◽  
pp. 664-672 ◽  
Author(s):  
Andrielle Castilho-Fernandes ◽  
Danilo Candido de Almeida ◽  
Aparecida Maria Fontes ◽  
Fernanda Ursoli Ferreira Melo ◽  
Virgínia Picanço-Castro ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Cell Line ◽  
Hepatic Stellate Cell ◽  
Stellate Cell ◽  
Human Hepatic Stellate Cell

scholarly journals CHARACTERIZATION OF AN IMMORTALIZED HEPATIC STELLATE CELL LINE ESTABLISHED FROM METALLOTHIONEIN-NULL MICE

2006 ◽  
Vol 31 (4) ◽  
pp. 391-398 ◽  
Author(s):  
Nobuhiko MIURA ◽  
Yoshitaka KANAYAMA ◽  
Wakako NAGAI ◽  
Tatsuya HASEGAWA ◽  
Yoshiyuki SEKO ◽  
...  
Keyword(s):  
Cell Line ◽  
Hepatic Stellate Cell ◽  
Stellate Cell

scholarly journals Genetic characteristics of the human hepatic stellate cell line LX–2

2012 ◽  
Vol 50 (01) ◽  
Author(s):  
R Weiskirchen ◽  
J Weimer ◽  
N Arnold ◽  
L Xu ◽  
SL Friedman ◽  
...  
Keyword(s):  
Cell Line ◽  
Hepatic Stellate Cell ◽  
Stellate Cell ◽  
Genetic Characteristics ◽  
Human Hepatic Stellate Cell
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